Expression of the phospho-β-glycosidase ArbZ from Lactobacillus delbrueckii subsp. lactis in Lactobacillus helveticus : substrate induction and catabolite repression

• Published in: Microbiology (Society for General Microbiology) Vol. 146; no. 8; pp. 1941 - 1948
• Main Authors: WEBER, B. A, KLEIN, J. R, HENRICH, B
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01.08.2000
• Subjects: Arbutin - metabolism; ArbZ protein; Bacterial Proteins - biosynthesis; Bacterial Proteins - genetics; Base Sequence; Biological and medical sciences; Biotechnology; Cloning, Molecular; DNA Primers - genetics; Enzyme Induction - drug effects; Fermentation; Fundamental and applied biological sciences. Psychology; Gene transfer; Genes, Bacterial; Genetic engineering; Genetic technics; Glucosides - metabolism; Glucosides - pharmacology; Glycoside Hydrolases - biosynthesis; Glycoside Hydrolases - genetics; Lactobacillus - drug effects; Lactobacillus - enzymology; Lactobacillus - genetics; Lactobacillus delbrueckii lactis; Lactobacillus helveticus; Methods. Procedures. Technologies; phospho- beta -glycosidase; substrate induction; Synthetic digonucleotides and genes. Sequencing; Transformation, Genetic; Lactic acid bacteria; Lactobacillus helveticus; Enzyme; Induction; Gene expression; Genetic transfer; Regulation(control); β-Glucosidase; Glycosidases; Bacteria; Hydrolases; Lactobacillaceae; Lactobacillus delbrueckii subsp. lactis; Recombinant protein; O-Glycosidases; Catabolic repression

ArbZ from Lactobacillus delbrueckii subsp. lactis was previously shown to enable utilization of the beta-glucoside arbutin by Escherichia coli. The arbZ gene was cloned and expressed in the industrially used beta-glucoside-negative strain Lactobacillus helveticus 3036(62). The transformants were abl...