Chitosan-poly (glycidyl methacrylate) copolymer for immobilization of urease

Urease was covalently attached onto chitosan-poly (GMA) by the introduction of epoxy groups to the support. The immobilized urease retained 82·15% of its specific activity. The pH optimum of the free and immobilized urease was unchanged, but the optimum temperature of the immobilized urease was high...

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Bibliographic Details
Published inProcess biochemistry (1991) Vol. 33; no. 6; pp. 595 - 600
Main Authors Chellapandian, M., Krishnan, M.R.V.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.08.1998
Subjects
chitosan
chitosan-poly (GMA)
immobilization
urease
chitosan
immobilization
chitosan-poly (GMA)
urease
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Summary:Urease was covalently attached onto chitosan-poly (GMA) by the introduction of epoxy groups to the support. The immobilized urease retained 82·15% of its specific activity. The pH optimum of the free and immobilized urease was unchanged, but the optimum temperature of the immobilized urease was higher than that of the free enzyme. Immobilization improved the thermal, pH and storage stability of the enzyme.
ISSN:1359-5113
1873-3298
DOI:10.1016/S0032-9592(98)80001-0