Reexamination of the ORAC assay: effect of metal ions

• Published in: Analytical and bioanalytical chemistry Vol. 400; no. 5; pp. 1451 - 1458
• Main Authors: Nkhili, E., Brat, P.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.05.2011; Springer
• Subjects: Analytical Chemistry; Antioxidants - metabolism; Ascorbic Acid - metabolism; Beverages - analysis; Biochemistry; Characterization and Evaluation of Materials; Chelating Agents - metabolism; Chemistry; Chemistry and Materials Science; Dehydroascorbic Acid; Edetic Acid - metabolism; Exact sciences and technology; Food Science; Laboratory Medicine; Monitoring/Environmental Analysis; Original Paper; Quercetin - metabolism; Reactive Oxygen Species - metabolism; Spectrometric and optical methods; ORAC; Phenolic compounds; Antioxidant activity; Metal chelator; Fruit juices; Ascorbic acid; Chemical analysis; Metal ion; Metal; Probe; Free radical; Chemical enrichment; Autoxidation; Oxidation; Hydrates; Trace analysis; Complexation; Oxygen; Stability; Sample; Use; Matrix effect; Foodstuff; Method; Antioxidant; EDTA; Fruit juice; Biological compound; Absorption spectrometry; Apple; Fluorescein; Quercetin; Chelating agent; Phenols; Xanthene dye

The oxygen radical absorbance capacity (ORAC) assay method has been employed extensively in the field of antioxidant and oxidative stress. It uses fluorescein as probe for oxidation by peroxyl radical. Hundreds of reports have been published on the use of this method to determine antioxidant capacit...