The use of qualitative and quantitative polymerase chain reactions for diagnosis of cytomegalovirus infections in bone marrow and kidney transplant recipients

• Published in: Revista da Sociedade Brasileira de Medicina Tropical Vol. 37; no. 2; pp. 158 - 164
• Main Authors: Marin, Lauro Juliano, Cunha, Aldo Albuquerque, Aquino, Victor Hugo, Figueiredo, Luiz Tadeu Moraes
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira de Medicina Tropical 01.03.2004; Sociedade Brasileira de Medicina Tropical - SBMT; Sociedade Brasileira de Medicina Tropical (SBMT)
• Subjects: Adolescent; Adult; Aged; Antigens, Viral - blood; Bone marrow; Bone marrow transplant; Bone Marrow Transplantation - adverse effects; Child; Child, Preschool; CMV infection; Cytomegalovirus - genetics; Cytomegalovirus - isolation & purification; Cytomegalovirus Infections - diagnosis; Deoxyribonucleic acid; DNA; DNA, Viral - blood; Female; Humans; Kidney transplant; Kidney transplantation; Kidney Transplantation - adverse effects; Leukocytes - virology; Male; Middle Aged; Polymerase Chain Reaction - methods; Qualitative PCR; Quantitative PCR; Sensitivity and Specificity; TROPICAL MEDICINE; Viral Load; Transplantados de rim; Transplantados de medula óssea; CMV infection; Kidney transplant; Infecção por CMV; Bone marrow transplant; PCR quantitativa; Quantitative PCR; PCR qualitativa; Qualitative PCR

The purpose of this work was to test a cytomegalovirus qualitative PCR and a semi-quantitative PCR on the determination of CMV load in leukocytes of bone marrow and kidney transplanted (RT) patients. Thirty three BMT and 35 RT patients participated of the study. The DNA was subjected to a qualitativ...