Single cell network profiling assay in bladder cancer

• Published in: Cytometry. Part A Vol. 83A; no. 4; pp. 386 - 395
• Main Authors: Covey, Todd M., Vira, Manish A., Westfall, Matt, Gulrajani, Michael, Cholankeril, Michelle, Okhunov, Zhamshid, Levey, Helen R., Marimpietri, Carol, Hawtin, Rachael, Fields, Scott Z., Cesano, Alessandra
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.04.2013
• Subjects: 1-Phosphatidylinositol 3-kinase; Aneuploidy; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; bladder cancer; cell signaling; Enzyme Inhibitors - pharmacology; Epithelial Cells - classification; Epithelial Cells - metabolism; Epithelial Cells - pathology; Extracellular Signal-Regulated MAP Kinases - genetics; Extracellular Signal-Regulated MAP Kinases - metabolism; Female; flow cytometry; Flow Cytometry - methods; Gene Expression Regulation, Neoplastic; Humans; Indazoles - pharmacology; multiparameter analysis; Phosphatidylinositol 3-Kinases - antagonists & inhibitors; Phosphatidylinositol 3-Kinases - genetics; Phosphatidylinositol 3-Kinases - metabolism; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; Signal Transduction; single cell network profiling; Single-Cell Analysis - methods; solid tumor; specimen source; Sulfonamides - pharmacology; Tumor Cells, Cultured; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - pathology

The aim of this study was to assess the feasibility of applying the single cell network profiling (SCNP) assay to the examination of signaling networks in epithelial cancer cells, using bladder washings from 29 bladder cancer (BC) and 15 nonbladder cancer (NC) subjects. This report describes the met...