Detection of antibodies to hepatitis C virus in U.S. blood donors
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| Published in | Journal of Clinical Microbiology Vol. 29; no. 3; pp. 551 - 556 |
|---|---|
| Main Authors | , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Washington, DC
American Society for Microbiology
01.03.1991
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| Subjects | |
| Online Access | Get full text |
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| AbstractList | An enzyme immunoassay (EIA) which utilizes a solid phase coated with a recombinant antigen (c100-3) derived from the hepatitis C virus (HCV) genome was evaluated for efficacy in the detection of antibodies to HCV (anti-HCV). The sensitivity of the antibody test was demonstrated by the detection of anti-HCV in a well-characterized panel of human specimens known to contain the infectious agent of non-A, non-B hepatitis. The specificity of the anti-HCV test was evaluated by testing 6,118 serum specimens from volunteer blood donors considered to be at low risk for exposure to HCV. The specificity of the anti-HCV EIA was demonstrated to be 99.56%, since 6,069 of 6,096 specimens from this low-risk group were nonreactive. A total of 49 (0.80%) of the 6,118 specimens were repeatedly reactive in the test, and 22 (46.81%) of the 47 specimens available for additional testing were confirmed as positive for antibodies to HCV c100-3. Among commercial plasma donors, 390 (10.49%) of 3,718 specimens were repeatedly reactive in the EIA. A total of 375 (97.40%) of the 385 specimens available for further testing were confirmed as positive. These limited data indicate that the prevalence of antibodies to HCV is 0.36% (22 confirmed positives among 6,118 specimens) among volunteer blood donors and 10.08% (375 confirmed positives among 3,718 specimens) among commercial plasma donors. The importance of confirmatory testing is discussed. An enzyme immunoassay (EIA) which utilizes a solid phase coated with a recombinant antigen (c100-3) derived from the hepatitis C virus (HCV) genome was evaluated for efficacy in the detection of antibodies to HCV (anti-HCV). The sensitivity of the antibody test was demonstrated by the detection of anti-HCV in a well-characterized panel of human specimens known to contain the infectious agent of non-A, non-B hepatitis. The specificity of the anti-HCV test was evaluated by testing 6,118 serum specimens from volunteer blood donors considered to be at low risk for exposure to HCV. The limited data indicate that the prevalence of antibodies to HCV is 0.36% (22 confirmed positives among 6,118 specimens) among volunteer blood donors and 10.08% (375 confirmed positives among 3,718 specimens) among commercial plasma donors. The importance of confirmatory testing is discussed. Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology. For an alternate route to JCM .asm.org, visit: JCM |
| Author | L Pendy X Alcalde K M Boardway R G Johnson G J Dawson K V Rote R R Lesniewski J L Stewart S G Devare R A Gutierrez |
| AuthorAffiliation | Abbott Diagnostics Division, Abbott Laboratories, North Chicago, Illinois 60064 |
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| Keywords | Performance evaluation Human Antibody United States Serology Enzyme immunoassay North America Blood Virus Sensitivity Specificity America Detection Non A non B hepatitis virus Blood donor |
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Mendeley... An enzyme immunoassay (EIA) which utilizes a solid phase coated with a recombinant antigen (c100-3) derived from the hepatitis C virus (HCV) genome was... |
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| StartPage | 551 |
| SubjectTerms | Amino Acid Sequence Antigens, Viral - chemistry Biological and medical sciences Blood Donors Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Hepacivirus - immunology Hepatitis Antibodies - blood Hepatitis C - diagnosis Hepatitis C - immunology Hepatitis C - transmission Hepatitis C Antibodies Humans Immunoenzyme Techniques Microbiology Molecular Sequence Data Peptides - chemistry Peptides - immunology Techniques used in virology Transfusion Reaction United States Virology |
| Title | Detection of antibodies to hepatitis C virus in U.S. blood donors |
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