Heat pretreatment differentially affects cardiac fatty acid accumulation during ischemia and postischemic reperfusion
Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, 6200 MD Maastricht, The Netherlands We investigated whether the cardioprotection induced by heat stress (HS) pretreatment is associated with mitigation of phospholipid degradation during the ischemic and/o...
Saved in:
Published in | American journal of physiology. Heart and circulatory physiology Vol. 280; no. 4; pp. H1736 - H1743 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.04.2001
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Department of Physiology, Cardiovascular Research Institute
Maastricht, Maastricht University, 6200 MD Maastricht, The
Netherlands
We investigated whether the cardioprotection
induced by heat stress (HS) pretreatment is associated with mitigation
of phospholipid degradation during the ischemic and/or
postischemic period. The hearts, isolated from control rats and
from heat-pretreated rats (42°C for 15 min) either 30 min (HS0.5-h)
or 24 h (HS24-h) earlier, were subjected to 45 min of no-flow
ischemia, followed by 45 min of reperfusion.
Unesterified arachidonic acid (AA) accumulation was taken as a measure
for phospholipid degradation. Significantly improved
postischemic ventricular functional recovery was only found in
the HS24-h group. During ischemia, AA accumulated comparably in
control and both HS groups. During reperfusion in control and HS0.5-h
hearts, AA further accumulated (control hearts from 82 ± 33 to
109 ± 51 nmol/g dry wt, not significant; HS-0.5h hearts from
52 ± 22 to 120 ± 53 nmol/g dry wt; P < 0.05). In contrast, AA was lower at the end of the reperfusion phase in
HS24-h hearts than at the end of the preceding ischemic period
(74 ± 18 vs. 46 ± 23 nmol/g dry wt; P < 0.05). Thus accelerated reperfusion-induced degradation of
phospholipids in control hearts is completely absent in HS24-h hearts.
Furthermore, the lack of functional improvement in HS0.5-h hearts is
also associated with a lack of beneficial effect on lipid homeostasis.
Therefore, it is proposed that enhanced membrane stability during
reperfusion is a key mediator in the heat-induced cardioprotection.
heat shock proteins; lipid metabolism; cardioprotection; enzyme
release |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0363-6135 1522-1539 |
DOI: | 10.1152/ajpheart.2001.280.4.H1736 |