Stoichiometry of Recombinant Heteromeric Glycine Receptors Revealed by a Pore-Lining Region Point Mutation

• Published in: Receptors & channels Vol. 9; no. 6; pp. 353 - 361
• Main Authors: Burzomato, Valeria, Groot-Kormelink, Paul J., Sivilotti, Lucia G., Beato, Marco
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 2003
• Subjects: Cell Line; Central Nervous System Stimulants - pharmacology; DNA, Complementary - metabolism; Dose-Response Relationship, Drug; Electrophysiology; Glycine - chemistry; Glycine - pharmacology; Glycine Receptor; Heterologous Expression; Humans; Mutagenesis, Site-Directed; Mutation; Picrotoxin; Picrotoxin - pharmacology; Plasmids - metabolism; Point Mutation; Protein Structure, Tertiary; Receptors, Glycine - chemistry; Receptors, Glycine - genetics; Receptors, Nicotinic - chemistry; Recombinant Proteins - chemistry; Single-Channel Recording; Site-Directed Mutagenesis; Subunit Stoichiometry; Transfection; Trigeminal Caudal Nucleus - metabolism

Heteromeric glycine receptors mediate synaptic inhibition in the caudal areas of the adult mammalian central nervous system (CNS). These channels resemble other receptors in the nicotinic superfamily in that they are pentamers, but may differ in that they contain α and β subunits in a 3:2 rather tha...