Constituents of Limonia acidissima inhibit LPS-induced nitric oxide production in BV-2 microglia

• Published in: Journal of enzyme inhibition and medicinal chemistry Vol. 25; no. 6; pp. 887 - 892
• Main Authors: Hyun Kim, Ki, Keun Ha, Sang, Yeou Kim, Sun, Joo Youn, Hyun, Ro Lee, Kang
• Format: Journal Article
• Language: English
• Published: England Informa Healthcare 01.12.2010; Taylor & Francis
• Subjects: Animals; Anti-Inflammatory Agents, Non-Steroidal - chemistry; Anti-Inflammatory Agents, Non-Steroidal - isolation & purification; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; BV-2 cells; Cell Line, Transformed; Cell Survival - drug effects; Drug Discovery; Enzyme Inhibitors - pharmacology; Lignans - chemistry; Lignans - isolation & purification; Lignans - pharmacology; Limonia acidissima; Lipopolysaccharides - toxicity; Mice; Microglia - drug effects; Microglia - metabolism; neoligna; Neuroprotective Agents - chemistry; Neuroprotective Agents - isolation & purification; Neuroprotective Agents - pharmacology; nitric oxide; Nitric Oxide - metabolism; Nitric Oxide Synthase - antagonists & inhibitors; Osmolar Concentration; Plant Bark - chemistry; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Rutaceae - chemistry
• DOI: 10.3109/14756360903514156
• ISSN: 1475-6366

The ethyl acetate (EtOAc) soluble fraction of the 85% ethanol (EtOH) extract of the dried bark of Limonia acidissima potently inhibited nitric oxide (NO) production in lipopolysaccharide (LPS) activated BV-2 cells, a microglial cell line. Bioassay-guided column chromatography separation afforded a new stereoisomer of neolignan, (7'E)-(7R,8S)-4-hydroxy-3,5'-dimethoxy-4',7-epoxy-8,3'-neolig-7'-en-9,9'-diyil diacetate (1), together with two known lignans, (+)-yangambin (2) and (+)-syringaresinol (3), three known triterpenoids, hederatriol (4), basic acid methyl ester (5), and 3β-hydroxyolean-12-en-11-one (6), and four known fatty acid derivatives, cascarillic acid (7), (+)-α-dimorphecolic acid (8), 8(R)-hydroxylinoleic acid (9), and (6Z,9Z,12Z)-pentadecatrienoic acid (10). The structure of the new compound 1 was elucidated by detailed analysis of spectroscopic data and circular dichroism (CD) spectroscopy. Compounds 1, 3-6, and 8-10 isolated from L. acidissima significantly reduced NO production in LPS-stimulated BV-2 microglia cells.