Diagnostic Host Gene Expression Analysis by Quantitative Reverse Transcription Loop-Mediated Isothermal Amplification to Discriminate between Bacterial and Viral Infections

• Published in: Clinical chemistry (Baltimore, Md.) Vol. 68; no. 4; pp. 550 - 560
• Main Authors: Remmel, Melissa C, Coyle, Sabrina M, Eshoo, Mark W, Sweeney, Timothy E, Rawling, David C
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 31.03.2022
• Subjects: Algorithms; Antibiotics; Bacteria; Bacterial diseases; Bacterial infections; Biomarkers; Blood; Etiology; Gene amplification; Gene expression; Infections; Medical diagnosis; Patients; Reverse transcription; Ribonucleic acid; RNA; Test systems; Viral infections; host response; molecular diagnostics; qRT-LAMP; LAMP; point-of-care; gene expression

Abstract Background Early and accurate diagnosis of acute infections can help minimize the overprescription of antibiotics and improve patient outcomes. Discrimination between bacterial and viral etiologies in acute infection based on changes in host gene expression has been described. Unfortunately...