A direct fluorescent signal transducer embedded in a DNA aptamer paves the way for versatile metal-ion detection

• Published in: Sensors and actuators. B, Chemical Vol. 304; p. 127376
• Main Authors: Raducanu, Vlad-Stefan, Rashid, Fahad, Zaher, Manal S., Li, Yanyan, Merzaban, Jasmeen S., Hamdan, Samir M.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.02.2020; Elsevier Science Ltd
• Subjects: Binding; Cy3 photoisomerization; Deoxyribonucleic acid; DNA; DNA aptamer; Embedding; Fluorescent dyes; Fluorescent signal transducer; G-quadruplex; Gene sequencing; Ion detectors; Ions; Metal ion detection; Metal ions; Potassium; Potassium sensor; Selectivity; Sensors; Metal ion detection; Potassium sensor; Cy3 photoisomerization; DNA aptamer; Fluorescent signal transducer; G-quadruplex

•We developed a transducing technology that does not require any external reporter.•We designed a DNA-based aptamer that directly transduces potassium ions concentration to fluorescence change.•The aptamer binds potassium ions and undergoes conformational changes that can be sensed by the incorporat...