Sequential Analysis of Gene Expression after an Osteogenic Stimulus - c-fos Expression Is Induced in Osteocytes

• Published in: Biochemical and biophysical research communications Vol. 217; no. 1; pp. 264 - 270
• Main Authors: Inaoka, T., Lean, J.M., Bessho, T., Chow, J.W.M., Mackay, A., Kokubo, T., Chambers, T.J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 05.12.1995
• Subjects: Animals; Bone Matrix - metabolism; Female; Gene Expression; Genes, fos; Growth Substances - genetics; In Situ Hybridization; Models, Biological; Osteocytes - metabolism; Osteogenesis - genetics; Proteins - genetics; Rats; Rats, Wistar; RNA, Messenger - genetics; RNA, Messenger - metabolism; Space life sciences; Stress, Mechanical

We have recently developed an experimental model whereby mechanical stimulation induces osteogenesis in the caudal vertebrae of rats. We used this model to assess expression of genes induced by mechanical loading. Bulk preparations of mRNA extracted after loading did not-show >2-fold increases in...