Indole acetic acid overproduction transformants of the rhizobacterium Pseudomonas sp. UW4

• Published in: Antonie van Leeuwenhoek Vol. 111; no. 9; pp. 1645 - 1660
• Main Authors: Duca, Daiana R., Rose, David R., Glick, Bernard R.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.09.2018; Springer Nature B.V
• Subjects: 1-Aminocyclopropane-1-carboxylate deaminase; Acetic acid; Acids; aseptic conditions; Bacteria; Biomedical and Life Sciences; Biosynthesis; canola; Elongation; enzyme activity; Gene expression; gene overexpression; Genes; genetic vectors; Gnotobiotic; Growth rate; indole acetic acid; Indoleacetic acid; Life Sciences; Medical Microbiology; Microbiology; Original Paper; Plant growth; plant growth-promoting rhizobacteria; Plant hormones; Plant Sciences; Polymerase chain reaction; Pseudomonas; quantitative polymerase chain reaction; Rhizobium; root growth; Soil Science & Conservation; Plant-growth-promoting bacteria; Rhizobacteria; Bacterial transformants; Indoleacetic acid biosynthesis; ACC deaminase

The plant growth-promoting rhizobacterium Pseudomonas sp. UW4 was transformed to increase the biosynthesis of the auxin, indole-3-acetic acid (IAA). Four native IAA biosynthesis genes from strain UW4 were individually cloned into an expression vector and introduced back into the wild-type strain. Qu...