2′,7′-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy

• Published in: Free radical research Vol. 44; no. 6; pp. 587 - 604
• Main Authors: Chen, Xiuping, Zhong, Zhangfeng, Xu, Zengtao, Chen, Lidian, Wang, Yitao
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd. (Informa Healthcare, Taylor & Francis AS) 2010; Taylor & Francis
• Subjects: Animals; DA; DCF; DCFH; Fluoresceins - chemistry; Fluoresceins - metabolism; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; fluorescent probe; Humans; Reactive Oxygen Species - analysis; ROS

Abstract Reactive oxygen species (ROS) are critically important chemical intermediates in biological studies, due to their multiple physiologically essential functions and their often pathologically deleterious effects. Consequently, it is vital that their presence in biological samples has to be qu...