An LC/MS/MS method for quantifying 25-hydroxyvitamin D3 in finger-prick plasma sample prepared by DEMECAL® micro plasma separation device: toward mail-in assessment of vitamin D status

• Published in: Analytical and bioanalytical chemistry Vol. 417; no. 17; pp. 3765 - 3775
• Main Authors: Maeda, Kodai, Sakamoto, Kyoka, Ito, Eiko, Sugimoto, Shinya, Tajima, Shingo, Sasahara, Takahisa, Kawamura, Shunji, Yaegashi, Kazuo, Higashi, Tatsuya
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2025; Springer Nature B.V
• Subjects: 25-Hydroxyvitamin D; Analytical Chemistry; Biochemistry; Blood; Blood plasma; Bone diseases; Calciferol; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Ethanol; Food Science; Heterogeneity; Ionization; Laboratory Medicine; Liquid chromatography; Mail; Mass spectrometry; Mass spectroscopy; Medical personnel; Metabolism; Metabolites; Monitoring/Environmental Analysis; Paper in Forefront; Plasma; Quantitative analysis; Room temperature; Sample preparation; Vitamin D; Vitamin deficiency; United States > US; Japan; Vitamin D status; Finger-prick blood; Micro plasma separation; Derivatization; LC/ESI–MS/MS

A mail-in assessment of vitamin D status will make it possible for the subjects to early detect any vitamin D insufficiency/deficiency at their homes, thus leading to a better management of their health. In this study, a liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI–...