Tumor-Related Gene Changes in Immunosuppressive Syrian Hamster Cholangiocarcinoma

• Published in: Pathology oncology research Vol. 19; no. 4; pp. 785 - 794
• Main Authors: Juasook, Amornrat, Aukkanimart, Ratchadawan, Boonmars, Thidarut, Sudsarn, Pakkayanee, Wonkchalee, Nadchanan, Laummaunwai, Porntip, Sriraj, Pranee
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.10.2013; Springer Nature B.V
• Subjects: Analysis of Variance; Animals; Anti-Inflammatory Agents - pharmacology; Bile Duct Neoplasms - genetics; Bile Duct Neoplasms - immunology; Bile Duct Neoplasms - metabolism; Bile Duct Neoplasms - pathology; Bile Ducts, Intrahepatic - pathology; Biomedical and Life Sciences; Biomedicine; Cancer Research; Catalase - genetics; Catalase - metabolism; Cholangiocarcinoma - genetics; Cholangiocarcinoma - immunology; Cholangiocarcinoma - metabolism; Cholangiocarcinoma - pathology; Cricetinae; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; Dimethylnitrosamine - pharmacology; Gene Expression Regulation, Neoplastic; Immunology; Interleukin-6 - genetics; Interleukin-6 - metabolism; Keratin-19 - chemistry; Keratin-19 - metabolism; Mesocricetus; Oncology; Opisthorchiasis; Opisthorchis viverrini; Pathology; Prednisolone - pharmacology; Proliferating Cell Nuclear Antigen - chemistry; Proliferating Cell Nuclear Antigen - metabolism; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Superoxide Dismutase-1; Immunohistochemistry; Tumor-related gene; Immunosuppressive; Cholangiocarcinogenesis
• ISSN: 1219-4956; 1532-2807
• DOI: 10.1007/s12253-013-9645-x

The results of a previous study demonstrated that prednisolone enhanced cholangiocarcinogenesis. Therefore, to clarify molecular changes during immunosuppressive cholangiocarcinogenesis, Syrian hamsters were divided into 8 groups: uninfected controls; immunosuppressed Syrian hamsters using prednisolone (P); normal Syrian hamsters administered N -nitrosodimethylamine (ND); immunosuppressed Syrian hamsters administered N -nitrosodimethylamine (ND is ); normal Syrian hamsters infected with Opisthorchis viverrini (OV); immunosuppressed Syrian hamsters infected with O. viverrini (OV is ); normal Syrian hamsters infected with O. viverrini and administered N -nitrosodimethylamine (CCA); and immunosuppressed Syrian hamsters infected with O. viverrini and administered N -nitrosodimethylamine (CCA is ). Syrian hamster livers were used for analysis of tumor-related gene expression and immunohistochemistry through cytokeratin 19 (CK19) and proliferating cell nuclear antigen (PCNA) staining. The tumor-related gene expression results show that CCA is groups at all time points exhibited upregulation of COX-2, IL-6, SOD1, CAT and iNOS and downregulation of p53, which correlated with the predominant expression of CK19 and PCNA in liver tissue. These results suggest that prednisolone enhances cholangiocarcinoma development, which was confirmed by molecular changes.