PS II model based analysis of transient fluorescence yield measured on whole leaves of Arabidopsis thaliana after excitation with light flashes of different energies

• Published in: BioSystems Vol. 103; no. 2; pp. 188 - 195
• Main Authors: Belyaeva, N.E., Schmitt, F.-J., Paschenko, V.Z., Riznichenko, G.Yu, Rubin, A.B., Renger, G.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.02.2011
• Subjects: Algae; Arabidopsis - metabolism; Arabidopsis thaliana; Computer Simulation; Dissipative energy losses; Electron transfer; Electron Transport - physiology; Fluorescence; Fluorescence yield; Kinetics; Model simulation; Models, Biological; Photosystem II; Photosystem II Protein Complex - metabolism; Plant Leaves - metabolism; Single turnover flash; Triplet carotenoid quenching; PQ; HL; Chl; Electron transfer; F0, FmSTF; Photosystem II; HS; Phe; PQH2; SFITFY; kZ; PS II; ΔΨ; QA and QB; PPFD; Model simulation; YZ; kHD; FL, FL(t); Single turnover flash; Dissipative energy losses; WOC; LED; ET; kP680+, k3Car; RC; kPhe; Fluorescence yield; 3Car; P680; kL(t); kF; Triplet carotenoid quenching
• DOI: 10.1016/j.biosystems.2010.09.014
• ISSN: 0303-2647

Our recently presented PS II model (Belyaeva et al., 2008) was improved in order to permit a consistent simulation of Single Flash Induced Transient Fluorescence Yield (SFITFY) traces that were earlier measured by Steffen et al. (2005) on whole leaves of Arabidopsis (A.) thaliana at four different energies of the actinic flash. As the essential modification, the shape of the actinic flash was explicitly taken into account assuming that an exponentially decaying rate simulates the time dependent excitation of PS II by the 10ns actinic flash. The maximum amplitude of this excitation exceeds that of the measuring light by 9 orders of magnitude. A very good fit of the SFITFY data was achieved in the time domain from 100ns to 10s for all actinic flash energies (the maximum energy of 7.5×1016 photons/(cm2flash) is set to 100%, the relative energies of weaker actinic flashes were of ∼8%, 4%, ∼1%). Our model allows the calculation and visualization of the transient PS II redox state populations ranging from the dark adapted state, via excitation energy and electron transfer steps induced by pulse excitation, followed by final relaxation into the stationary state eventually attained under the measuring light. It turned out that the rate constants of electron transfer steps are invariant to intensity of the actinic laser flash. In marked contrast, an increase of the actinic flash energy by more than two orders of magnitude from 5.4×1014 photons/(cm2flash) to 7.5×1016 photons/(cm2flash), leads to an increase of the extent of fluorescence quenching due to carotenoid triplet (3Car) formation by a factor of 14 and of the recombination reaction between reduced primary pheophytin (Phe−) and P680+ by a factor of 3 while the heat dissipation in the antenna complex remains virtually constant. The modified PS II model offers new opportunities to compare electron transfer and dissipative parameters for different species (e.g. for the green algae and the higher plant) under varying illumination conditions.