Effects of l-arabinose efflux on λ Red recombination-mediated gene knockout in multiple-antimicrobial-resistant Salmonella enterica serovar Choleraesuis

• Published in: Archives of microbiology Vol. 200; no. 2; pp. 219 - 225
• Main Authors: Liao, Shi-Wei, Lee, Jen-Jie, Ptak, Christopher P., Wu, Ying-Chen, Hsuan, Shih-Ling, Kuo, Chih-Jung, Chen, Ter-Hsin
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.03.2018; Springer Nature B.V
• Subjects: Antibiotic resistance; Antibiotics; Antiinfectives and antibacterials; Antimicrobial agents; Arabinose; bacteriophages; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; dose response; Ecology; Efflux; fluorescent dyes; Functional analysis; Gene expression; gene targeting; Genetic analysis; genetic engineering; Life Sciences; Microbial Ecology; Microbiology; Original Paper; Recombinase; Recombination; Salmonella; Salmonella choleraesuis Choleraesuis; Strains (organisms); Substrates; Swine; transporters; Virulence; Choleraesuis; Arabinose; λ Red recombination; Antimicrobial resistance; Gene knockout; Salmonella Choleraesuis; L-Arabinose

In this study, six swine-derived multiple-antimicrobial-resistant (MAR) strains of Salmonella Choleraesuis ( S . Choleraesuis) were demonstrated to possess higher efflux pump activity than the wild-type (WT). l -Arabinose, a common inducer for gene expression, modulated S . Choleraesuis efflux pump...