Inhibition of β-Amylase Activity by Calcium, Magnesium and Zinc Ions Determined by Spectrophotometry and Isothermal Titration Calorimetry

• Published in: Journal of enzyme inhibition and medicinal chemistry Vol. 19; no. 2; pp. 157 - 160
• Main Authors: Umar Dahot, M., Saboury, A.A., Moosavi-Movahedi, A.A.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.04.2004
• Subjects: beta-Amylase - antagonists & inhibitors; Calcium - pharmacology; Calorimetry - methods; Cations, Divalent; Inhibition; Isothermal titration calorimetry; Kinetics; Magnesium - pharmacology; Metal ions; Spectrophotometry; Zinc - pharmacology; β-Amylase

The inhibition effect of metal ions on beta amylase activity was studied. The inhibitor-binding constant (Ki) was determined by spectrophotometric and isothermal titration calorimetric (ITC) methods. The binding of calcium, magnesium and zinc ion as inhibitors at the active site of barley beta amyla...