Relationship between molecular weight, monosaccharide composition and immunobiologic activity of Astragalus polysaccharides

• Published in: Glycoconjugate journal Vol. 33; no. 5; pp. 755 - 761
• Main Authors: Jiang, Yiping, Qi, Xiaohui, Gao, Kai, Liu, Wenjun, Li, Na, Cheng, Ningbo, Ding, Gang, Huang, Wenzhe, Wang, Zhenzhong, Xiao, Wei
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2016; Springer Nature B.V
• Subjects: Animals; Astragalus; Astragalus Plant - chemistry; Biochemistry; Biomedical and Life Sciences; Carbohydrate Conformation; Cell Proliferation - drug effects; Ethanol; Female; Immunology; Life Sciences; Lymphocytes - immunology; Mice; Mice, Inbred BALB C; Molecular biology; Monosaccharides - chemistry; Monosaccharides - pharmacology; Original Article; Pathology; Pneumoviridae; Polysaccharides - chemistry; Polysaccharides - pharmacology; Astragalus polysaccharide; Monosaccharide composition; Immunobiologic activity; Molecular weight

Four Astragalus polysaccharides (APS1-APS4) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation with 20 %, 40 %, 60 % and 80 % ethanol, respectively. The total sugar content was measured by sulfuric acid-phenol method. Their molecular weight was determi...