Assessment of genotoxicity amongst smokers, alcoholics, and tobacco chewers of North India using micronucleus assay and urinary 8-hydroxyl−2′-deoxyguanosine, as biomarkers

• Published in: Environmental monitoring and assessment Vol. 189; no. 8; p. 391
• Main Authors: Rana, S. V. S., Verma, Yeshvandra, Singh, Gagan Deep
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.08.2017; Springer Nature B.V
• Subjects: 8-Hydroxy-2'-Deoxyguanosine; 8-Hydroxydeoxyguanosine; Acetaldehyde; Adult; alcohol abuse; alcohol drinking; Alcoholics; Alcoholics - statistics & numerical data; Alcoholism; Alcohols; Assaying; Atmospheric Protection/Air Quality Control/Air Pollution; Biomarkers; Biomarkers - urine; Carcinogens; Cells; Chewing; Cigarette smoke; cigarettes; Creatinine; Deoxyguanosine; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - urine; DNA Damage; Earth and Environmental Science; Ecology; Ecotoxicology; Environment; Environmental Exposure - statistics & numerical data; Environmental Management; Environmental Monitoring; Environmental science; Epithelium; Genotoxicity; Habits; human population; Human populations; Humans; India; Male; males; Manganese; Mastication; Micronucleus Tests; Middle Aged; Monitoring/Environmental Analysis; Mucosa; Nitrosamines; Oxidative Stress; Population studies; Smoke; Smoking; Statistical analysis; Statistical methods; Tobacco; Tobacco Smoke Pollution - statistics & numerical data; Tobacco, Smokeless; Urine; India; Alcohol; Tobacco; Micronucleus assay; 8-Hydroxyl-2′-deoxyguanosine; Genotoxicity; Smoking

The main objective of the present study was to screen the genotoxicity caused by individual and combined habits of smoking, tobacco chewing, and alcohol consumption in human population of North India. Study recruited 67 male subjects aged 25 to 65 years. Buccal mucosal cells were subjected to micron...