Additional Member of the Protein-Tyrosine Kinase Family: The src- and lck-Related Protooncogene c-tkl
We report the isolation and nucleotide sequence of a 3.7-kilobase (kb) cDNA clone from chicken spleen corresponding to a previously undescribed member of the src family of protooncogenes. It encodes a protein with a C-terminal domain related to the src family of protein-tyrosine kinases (EC 2.7.1.11...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 84; no. 24; pp. 8778 - 8782 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
National Academy of Sciences of the United States of America
01.12.1987
National Acad Sciences |
Subjects | |
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Abstract | We report the isolation and nucleotide sequence of a 3.7-kilobase (kb) cDNA clone from chicken spleen corresponding to a previously undescribed member of the src family of protooncogenes. It encodes a protein with a C-terminal domain related to the src family of protein-tyrosine kinases (EC 2.7.1.112) and, among these, has most significant homology to the lck gene isolated from a murine leukemia virus-induced thymoma cell line. The gene is therefore referred to as c-tkl for cellular tyrosine kinase related to lck. Analysis of genomic DNA reveals that c-tkl is a chromosomal locus distinct from c-src and c-lck. Furthermore, the size of c-tkl mRNA as well as its pattern of expression indicates that it is not the chicken homologue of lck but a different gene. A 3.8-kb transcript of the c-tkl gene, identical to the size determined for c-src mRNA, was observed in cultured chicken embryo fibroblasts and in chicken spleen and brain. In contrast, detection of a definite c-src mRNA signal with mRNA from spleen was not possible under the hybridization conditions employed when the 5′ end of v-src was used as the probe, and none of the 11 clones obtained from the cDNA library corresponded to a c-src transcript. Thus previous studies of c-src mRNA expression in spleen may have actually detected c-tkl transcripts. |
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AbstractList | We report the isolation and nucleotide sequence of a 3.7-kilobase (kb) cDNA clone from chicken spleen corresponding to a previously undescribed member of the src family of protooncogenes. It encodes a protein with a C-terminal domain related to the src family of protein-tyrosine kinases (EC 2.7.1.112) and, among these, has most significant homology to the lck gene isolated from a murine leukemia virus-induced thymoma cell line. The gene is therefore referred to as c-tkl for cellular tyrosine kinase related to lck. Analysis of genomic DNA reveals that c-tkl is a chromosomal locus distinct from c-src and c-lck. Furthermore, the size of c-tkl mRNA as well as its pattern of expression indicates that it is not the chicken homologue of lck but a different gene. A 3.8-kb transcript of the c-tkl gene, identical to the size determined for c-src mRNA, was observed in cultured chicken embryo fibroblasts and in chicken spleen and brain. In contrast, detection of a definite c-src mRNA signal with mRNA from spleen was not possible under the hybridization conditions employed when the 5' end of v-src was used as the probe, and none of the 11 clones obtained from the cDNA library corresponded to a c-src transcript. Thus previous studies of c-src mRNA expression in spleen may have actually detected c-tkl transcripts. |
Author | Bruck, Claudine Strebhardt, Klaus Mullins, James I. Rübsamen-Waigmann, Helga |
AuthorAffiliation | Chemotherapeutisches Forschungsinstitut, Georg-Speyer-Haus, Frankfurt, Federal Republic of Germany |
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Keywords | Characterization Spleen Vertebrata Animal C-Onc gene Primary structure Identification Chicken Aves Molecular cloning Protein-tyrosine kinase |
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Snippet | We report the isolation and nucleotide sequence of a 3.7-kilobase (kb) cDNA clone from chicken spleen corresponding to a previously undescribed member of the... |
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SubjectTerms | Amino Acid Sequence Amino acids Analytical, structural and metabolic biochemistry Animals Base Sequence Biological and medical sciences Cells, Cultured Chickens Chickens - genetics Cloning, Molecular Complementary DNA DNA Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Generally accepted auditing standards Genes Messenger RNA Molecular Sequence Data Multigene Family Nucleotides Protein-Tyrosine Kinases - genetics Proto-Oncogene Proteins - genetics RNA RNA, Messenger - genetics Spleen Tissue Distribution Transferases |
Title | Additional Member of the Protein-Tyrosine Kinase Family: The src- and lck-Related Protooncogene c-tkl |
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