Quantification of total cholesterol in human milk by gas chromatography

• Published in: Journal of separation science Vol. 41; no. 8; pp. 1805 - 1811
• Main Authors: Beggio, Maurizio, Cruz‐Hernandez, Cristina, Golay, Pierre‐Alain, Lee, Le Ye, Giuffrida, Francesca
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.04.2018
• Subjects: Baby foods; Cholesterol; Cholesterol - analysis; Chromatography; Chromatography, Gas; desmosterol; Flame ionization; Flame ionization detectors; Gas chromatography; human milk; Humans; Infrared radiation; Ionization; Ionization counters; Milk; Milk, Human - chemistry; Nutrients; Potassium hydroxides; Reproducibility; cholesterol; human milk; gas chromatography; desmosterol

Human milk provides the key nutrients necessary for infant growth and development. The objective of this study was to develop and validate a method to analyze the cholesterol content in liquid human milk samples along lactation. Direct saponification of the sample using ethanolic potassium hydroxide...