Rnf32 is not essential for spermatogenesis and male fertility in mice
Ring finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein-protein interactions. The -encoded protein contains two such motifs and is predominantly expressed in the testes and ovaries, suggesting that its expression may be regulated by elem...
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| Published in | PeerJ (San Francisco, CA) Vol. 13; p. e19794 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
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| Abstract | Ring finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein-protein interactions. The
-encoded protein contains two such motifs and is predominantly expressed in the testes and ovaries, suggesting that its expression may be regulated by elements within the
promoter region.
is active during spermatogenesis, mainly in spermatocytes and spermatids, indicating a potential role in sperm development.
We established an
knockout (
) mouse model using CRISPR/Cas9 technology. Gene expression was analyzed
reverse transcription quantitative polymerase chain reaction (RT-qPCR). Testicular and epididymal phenotypes were assessed through histological and immunofluorescence staining, and fertility and sperm motility were evaluated.
Here, we successfully established an
knockout mouse model using CRISPR/Cas9 technology. Surprisingly, male
mice exhibited normal fertility, with no significant differences in testicular and epididymal histology, spermatogenesis, sperm count, or motility compared to
mice. These findings suggest that
may not be essential for male fertility in mice, and its potential functions warrant further investigation. |
|---|---|
| AbstractList | BackgroundRing finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein–protein interactions. The Rnf32-encoded protein contains two such motifs and is predominantly expressed in the testes and ovaries, suggesting that its expression may be regulated by elements within the Rnf32 promoter region. Rnf32 is active during spermatogenesis, mainly in spermatocytes and spermatids, indicating a potential role in sperm development. MethodsWe established an Rnf32 knockout (Rnf32−/−) mouse model using CRISPR/Cas9 technology. Gene expression was analyzed via reverse transcription quantitative polymerase chain reaction (RT-qPCR). Testicular and epididymal phenotypes were assessed through histological and immunofluorescence staining, and fertility and sperm motility were evaluated. ResultsHere, we successfully established an Rnf32 knockout mouse model using CRISPR/Cas9 technology. Surprisingly, male Rnf32−/− mice exhibited normal fertility, with no significant differences in testicular and epididymal histology, spermatogenesis, sperm count, or motility compared to Rnf32+/+ mice. These findings suggest that Rnf32 may not be essential for male fertility in mice, and its potential functions warrant further investigation. Background Ring finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein–protein interactions. The Rnf32-encoded protein contains two such motifs and is predominantly expressed in the testes and ovaries, suggesting that its expression may be regulated by elements within the Rnf32 promoter region. Rnf32 is active during spermatogenesis, mainly in spermatocytes and spermatids, indicating a potential role in sperm development. Methods We established an Rnf32 knockout (Rnf32−/−) mouse model using CRISPR/Cas9 technology. Gene expression was analyzed via reverse transcription quantitative polymerase chain reaction (RT-qPCR). Testicular and epididymal phenotypes were assessed through histological and immunofluorescence staining, and fertility and sperm motility were evaluated. Results Here, we successfully established an Rnf32 knockout mouse model using CRISPR/Cas9 technology. Surprisingly, male Rnf32−/− mice exhibited normal fertility, with no significant differences in testicular and epididymal histology, spermatogenesis, sperm count, or motility compared to Rnf32+/+ mice. These findings suggest that Rnf32 may not be essential for male fertility in mice, and its potential functions warrant further investigation. Ring finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein-protein interactions. The -encoded protein contains two such motifs and is predominantly expressed in the testes and ovaries, suggesting that its expression may be regulated by elements within the promoter region. is active during spermatogenesis, mainly in spermatocytes and spermatids, indicating a potential role in sperm development. We established an knockout ( ) mouse model using CRISPR/Cas9 technology. Gene expression was analyzed reverse transcription quantitative polymerase chain reaction (RT-qPCR). Testicular and epididymal phenotypes were assessed through histological and immunofluorescence staining, and fertility and sperm motility were evaluated. Here, we successfully established an knockout mouse model using CRISPR/Cas9 technology. Surprisingly, male mice exhibited normal fertility, with no significant differences in testicular and epididymal histology, spermatogenesis, sperm count, or motility compared to mice. These findings suggest that may not be essential for male fertility in mice, and its potential functions warrant further investigation. Background Ring finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein-protein interactions. The Rnf32-encoded protein contains two such motifs and is predominantly expressed in the testes and ovaries, suggesting that its expression may be regulated by elements within the Rnf32 promoter region. Rnf32 is active during spermatogenesis, mainly in spermatocytes and spermatids, indicating a potential role in sperm development. Methods We established an Rnf32 knockout (Rnf32.sup.-/- ) mouse model using CRISPR/Cas9 technology. Gene expression was analyzed via reverse transcription quantitative polymerase chain reaction (RT-qPCR). Testicular and epididymal phenotypes were assessed through histological and immunofluorescence staining, and fertility and sperm motility were evaluated. Results Here, we successfully established an Rnf32 knockout mouse model using CRISPR/Cas9 technology. Surprisingly, male Rnf32.sup.-/- mice exhibited normal fertility, with no significant differences in testicular and epididymal histology, spermatogenesis, sperm count, or motility compared to Rnf32.sup.+/+ mice. These findings suggest that Rnf32 may not be essential for male fertility in mice, and its potential functions warrant further investigation. Ring finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein-protein interactions. The Rnf32-encoded protein contains two such motifs and is predominantly expressed in the testes and ovaries, suggesting that its expression may be regulated by elements within the Rnf32 promoter region. Rnf32 is active during spermatogenesis, mainly in spermatocytes and spermatids, indicating a potential role in sperm development.BackgroundRing finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein-protein interactions. The Rnf32-encoded protein contains two such motifs and is predominantly expressed in the testes and ovaries, suggesting that its expression may be regulated by elements within the Rnf32 promoter region. Rnf32 is active during spermatogenesis, mainly in spermatocytes and spermatids, indicating a potential role in sperm development.We established an Rnf32 knockout (Rnf32 -/-) mouse model using CRISPR/Cas9 technology. Gene expression was analyzed via reverse transcription quantitative polymerase chain reaction (RT-qPCR). Testicular and epididymal phenotypes were assessed through histological and immunofluorescence staining, and fertility and sperm motility were evaluated.MethodsWe established an Rnf32 knockout (Rnf32 -/-) mouse model using CRISPR/Cas9 technology. Gene expression was analyzed via reverse transcription quantitative polymerase chain reaction (RT-qPCR). Testicular and epididymal phenotypes were assessed through histological and immunofluorescence staining, and fertility and sperm motility were evaluated.Here, we successfully established an Rnf32 knockout mouse model using CRISPR/Cas9 technology. Surprisingly, male Rnf32 -/- mice exhibited normal fertility, with no significant differences in testicular and epididymal histology, spermatogenesis, sperm count, or motility compared to Rnf32 +/+ mice. These findings suggest that Rnf32 may not be essential for male fertility in mice, and its potential functions warrant further investigation.ResultsHere, we successfully established an Rnf32 knockout mouse model using CRISPR/Cas9 technology. Surprisingly, male Rnf32 -/- mice exhibited normal fertility, with no significant differences in testicular and epididymal histology, spermatogenesis, sperm count, or motility compared to Rnf32 +/+ mice. These findings suggest that Rnf32 may not be essential for male fertility in mice, and its potential functions warrant further investigation. Ring finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein-protein interactions. The Rnf32-encoded protein contains two such motifs and is predominantly expressed in the testes and ovaries, suggesting that its expression may be regulated by elements within the Rnf32 promoter region. Rnf32 is active during spermatogenesis, mainly in spermatocytes and spermatids, indicating a potential role in sperm development. We established an Rnf32 knockout (Rnf32.sup.-/- ) mouse model using CRISPR/Cas9 technology. Gene expression was analyzed via reverse transcription quantitative polymerase chain reaction (RT-qPCR). Testicular and epididymal phenotypes were assessed through histological and immunofluorescence staining, and fertility and sperm motility were evaluated. Here, we successfully established an Rnf32 knockout mouse model using CRISPR/Cas9 technology. Surprisingly, male Rnf32.sup.-/- mice exhibited normal fertility, with no significant differences in testicular and epididymal histology, spermatogenesis, sperm count, or motility compared to Rnf32.sup.+/+ mice. These findings suggest that Rnf32 may not be essential for male fertility in mice, and its potential functions warrant further investigation. |
| ArticleNumber | e19794 |
| Audience | Academic |
| Author | Zhu, Yunfei Yin, Yufeng Cui, Yiqiang Kong, Hao Zeng, Ni |
| Author_xml | – sequence: 1 givenname: Hao surname: Kong fullname: Kong, Hao organization: State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, China – sequence: 2 givenname: Yufeng surname: Yin fullname: Yin, Yufeng organization: State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, China – sequence: 3 givenname: Ni surname: Zeng fullname: Zeng, Ni organization: State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, China – sequence: 4 givenname: Yunfei surname: Zhu fullname: Zhu, Yunfei organization: State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, China – sequence: 5 givenname: Yiqiang surname: Cui fullname: Cui, Yiqiang organization: State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, China |
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| Cites_doi | 10.1126/sciadv.aav4832 10.1016/j.beem.2018.03.006 10.1007/s11033-022-07512-z 10.1093/humrep/del279 10.7717/peerj.16558 10.1016/j.bbrc.2023.07.013 10.1038/s41586-022-05508-0 10.1038/nrg911 10.1210/jcem.84.10.6101 10.1007/978-0-387-09597-4_1 10.1016/j.isci.2021.103396 10.1016/s0168-9525(00)02140-5 10.1038/s41422-018-0074-y 10.1095/biolreprod.102.012609 10.7150/ijbs.60384 10.1186/s12864-024-10298-y 10.1007/s11427-012-4411-y 10.1152/physrev.00013.2015 10.1530/REP-13-0420 10.1038/aja.2012.81 10.1016/j.cub.2017.07.067 10.1006/bbrc.2002.6612 10.1093/humupd/dmx021 10.1074/jbc.REV120.008029 10.2174/138161209789271825 10.1016/j.mce.2020.110956 10.1111/j.1745-7262.2007.00285.x 10.1016/j.bbrc.2022.01.084 10.1002/mrd.20026 |
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| References | Cooke (10.7717/peerj.19794/ref-8) 2002; 3 Lv (10.7717/peerj.19794/ref-23) 2022; 596 McLachlan (10.7717/peerj.19794/ref-24) 2007; 22 Dacheux (10.7717/peerj.19794/ref-10) 2014; 147 Zou (10.7717/peerj.19794/ref-30) 2021; 24 Cooper (10.7717/peerj.19794/ref-9) 2007; 9 Yu (10.7717/peerj.19794/ref-29) 2003; 69 Guo (10.7717/peerj.19794/ref-17) 2004; 67 Chen (10.7717/peerj.19794/ref-5) 2024; 25 Gilbert (10.7717/peerj.19794/ref-14) 2018; 32 Azhar (10.7717/peerj.19794/ref-2) 2021; 17 Chen (10.7717/peerj.19794/ref-7) 2018; 28 Ickowicz (10.7717/peerj.19794/ref-20) 2012; 14 Abe (10.7717/peerj.19794/ref-1) 1991; 66 Chasapis (10.7717/peerj.19794/ref-4) 2009; 15 Hackstein (10.7717/peerj.19794/ref-18) 2000; 16 Fon Tacer (10.7717/peerj.19794/ref-13) 2019; 5 Saint-Dizier (10.7717/peerj.19794/ref-27) 2020; 516 Van Baren (10.7717/peerj.19794/ref-28) 2002; 292 Li (10.7717/peerj.19794/ref-21) 2022; 612 Barratt (10.7717/peerj.19794/ref-3) 2017; 23 Griswold (10.7717/peerj.19794/ref-16) 2016; 96 Gong (10.7717/peerj.19794/ref-15) 2013; 56 Hess (10.7717/peerj.19794/ref-19) 2008; 636 Florke Gee (10.7717/peerj.19794/ref-12) 2020; 295 Li (10.7717/peerj.19794/ref-22) 2023; 675 Meng (10.7717/peerj.19794/ref-25) 2023; 11 De Kretser (10.7717/peerj.19794/ref-11) 1999; 84 Chen (10.7717/peerj.19794/ref-6) 2022; 49 Nishimura (10.7717/peerj.19794/ref-26) 2017; 27 |
| References_xml | – volume: 5 start-page: eaav4832 year: 2019 ident: 10.7717/peerj.19794/ref-13 article-title: MAGE cancer-testis antigens protect the mammalian germline under environmental stress publication-title: Science Advances doi: 10.1126/sciadv.aav4832 – volume: 32 start-page: 219 year: 2018 ident: 10.7717/peerj.19794/ref-14 article-title: Mouse models for the analysis of gonadotropin secretion and action publication-title: Best Practice & Research. Clinical Endocrinology & Metabolism doi: 10.1016/j.beem.2018.03.006 – volume: 49 start-page: 7287 year: 2022 ident: 10.7717/peerj.19794/ref-6 article-title: Fbxw17 is dispensable for viability and fertility in mice publication-title: Molecular Biology Reports doi: 10.1007/s11033-022-07512-z – volume: 22 start-page: 2 year: 2007 ident: 10.7717/peerj.19794/ref-24 article-title: Histological evaluation of the human testis–approaches to optimizing the clinical value of the assessment: mini review publication-title: Human Reproduction doi: 10.1093/humrep/del279 – volume: 11 start-page: e16558 year: 2023 ident: 10.7717/peerj.19794/ref-25 article-title: Slc26a1 is not essential for spermatogenesis and male fertility in mice publication-title: PeerJ doi: 10.7717/peerj.16558 – volume: 675 start-page: 46 year: 2023 ident: 10.7717/peerj.19794/ref-22 article-title: MAGE-B4, a binding partner of PRAMEF12, is dispensable for spermatogenesis and male fertility in mice publication-title: Biochemical and Biophysical Research Communications doi: 10.1016/j.bbrc.2023.07.013 – volume: 612 start-page: 725 year: 2022 ident: 10.7717/peerj.19794/ref-21 article-title: A male germ-cell-specific ribosome controls male fertility publication-title: Nature doi: 10.1038/s41586-022-05508-0 – volume: 3 start-page: 790 year: 2002 ident: 10.7717/peerj.19794/ref-8 article-title: Mouse models of male infertility publication-title: Nature Reviews. Genetics doi: 10.1038/nrg911 – volume: 84 start-page: 3443 year: 1999 ident: 10.7717/peerj.19794/ref-11 article-title: Infertility in men: recent advances and continuing controversies publication-title: The Journal of Clinical Endocrinology and Metabolism doi: 10.1210/jcem.84.10.6101 – volume: 636 start-page: 1 year: 2008 ident: 10.7717/peerj.19794/ref-19 article-title: Spermatogenesis and cycle of the seminiferous epithelium publication-title: Advances in Experimental Medicine and Biology doi: 10.1007/978-0-387-09597-4_1 – volume: 24 start-page: 103396 year: 2021 ident: 10.7717/peerj.19794/ref-30 article-title: Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis publication-title: iScience doi: 10.1016/j.isci.2021.103396 – volume: 16 start-page: 565 year: 2000 ident: 10.7717/peerj.19794/ref-18 article-title: Towards an understanding of the genetics of human male infertility: lessons from flies publication-title: Trends in Genetics: TIG doi: 10.1016/s0168-9525(00)02140-5 – volume: 28 start-page: 879 year: 2018 ident: 10.7717/peerj.19794/ref-7 article-title: Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis publication-title: Cell Research doi: 10.1038/s41422-018-0074-y – volume: 66 start-page: 286 issue: 3 year: 1991 ident: 10.7717/peerj.19794/ref-1 article-title: The cycle of the seminiferous epithelium and stages in spermatogenesis in dd-mice publication-title: [Hokkaido Igaku Zasshi] The Hokkaido Journal of Medical Science – volume: 69 start-page: 37 year: 2003 ident: 10.7717/peerj.19794/ref-29 article-title: Gene expression profiles in different stages of mouse spermatogenic cells during spermatogenesis publication-title: Biology of Reproduction doi: 10.1095/biolreprod.102.012609 – volume: 17 start-page: 2487 year: 2021 ident: 10.7717/peerj.19794/ref-2 article-title: Towards post-meiotic sperm production: genetic insight into human infertility from mouse models publication-title: International Journal of Biological Sciences doi: 10.7150/ijbs.60384 – volume: 25 start-page: 395 year: 2024 ident: 10.7717/peerj.19794/ref-5 article-title: Multiple transcriptome analyses reveal mouse testis developmental dynamics publication-title: BMC Genomics doi: 10.1186/s12864-024-10298-y – volume: 56 start-page: 1 year: 2013 ident: 10.7717/peerj.19794/ref-15 article-title: Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing publication-title: Science China. Life Sciences doi: 10.1007/s11427-012-4411-y – volume: 96 start-page: 1 year: 2016 ident: 10.7717/peerj.19794/ref-16 article-title: Spermatogenesis: the commitment to meiosis publication-title: Physiological Reviews doi: 10.1152/physrev.00013.2015 – volume: 147 start-page: R27 year: 2014 ident: 10.7717/peerj.19794/ref-10 article-title: New insights into epididymal function in relation to sperm maturation publication-title: Reproduction doi: 10.1530/REP-13-0420 – volume: 14 start-page: 816 year: 2012 ident: 10.7717/peerj.19794/ref-20 article-title: Mechanism of sperm capacitation and the acrosome reaction: role of protein kinases publication-title: Asian Journal of Andrology doi: 10.1038/aja.2012.81 – volume: 27 start-page: R988 year: 2017 ident: 10.7717/peerj.19794/ref-26 article-title: Spermatogenesis publication-title: Current Biology: CB doi: 10.1016/j.cub.2017.07.067 – volume: 292 start-page: 58 year: 2002 ident: 10.7717/peerj.19794/ref-28 article-title: A double RING-H2 domain in RNF32, a gene expressed during sperm formation publication-title: Biochemical and Biophysical Research Communications doi: 10.1006/bbrc.2002.6612 – volume: 23 start-page: 660 year: 2017 ident: 10.7717/peerj.19794/ref-3 article-title: The diagnosis of male infertility: an analysis of the evidence to support the development of global WHO guidance-challenges and future research opportunities publication-title: Human Reproduction Update doi: 10.1093/humupd/dmx021 – volume: 295 start-page: 16121 year: 2020 ident: 10.7717/peerj.19794/ref-12 article-title: Emerging roles of the MAGE protein family in stress response pathways publication-title: The Journal of Biological Chemistry doi: 10.1074/jbc.REV120.008029 – volume: 15 start-page: 3716 year: 2009 ident: 10.7717/peerj.19794/ref-4 article-title: RING finger E(3) ubiquitin ligases: structure and drug discovery publication-title: Current Pharmaceutical Design doi: 10.2174/138161209789271825 – volume: 516 start-page: 110956 year: 2020 ident: 10.7717/peerj.19794/ref-27 article-title: Sperm interactions with the female reproductive tract: a key for successful fertilization in mammals publication-title: Molecular and Cellular Endocrinology doi: 10.1016/j.mce.2020.110956 – volume: 9 start-page: 533 year: 2007 ident: 10.7717/peerj.19794/ref-9 article-title: Sperm maturation in the epididymis: a new look at an old problem publication-title: Asian Journal of Andrology doi: 10.1111/j.1745-7262.2007.00285.x – volume: 596 start-page: 71 year: 2022 ident: 10.7717/peerj.19794/ref-23 article-title: WDFY1, a WD40 repeat protein, is not essential for spermatogenesis and male fertility in mice publication-title: Biochemical and Biophysical Research Communications doi: 10.1016/j.bbrc.2022.01.084 – volume: 67 start-page: 264 year: 2004 ident: 10.7717/peerj.19794/ref-17 article-title: Stage-specific and tissue-specific expression characteristics of differentially expressed genes during mouse spermatogenesis publication-title: Molecular Reproduction and Development doi: 10.1002/mrd.20026 |
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| Snippet | Ring finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein-protein interactions. The
-encoded... Background Ring finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein-protein interactions. The... Ring finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein-protein interactions. The Rnf32-encoded... BackgroundRing finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein–protein interactions. The... Background Ring finger motifs are found in a variety of proteins with diverse functions, often involved in protein-DNA or protein–protein interactions. The... |
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| SubjectTerms | Andrology Animals Biochemistry Carbon dioxide CRISPR CRISPR-Cas Systems CRISPR/Cas9 Developmental Biology Euthanasia Females Fertility Fertility - genetics Gametocytes Gene expression Genetic aspects Genetic transcription Histology Immunofluorescence Infertility Male Males Mice Mice, Knockout Molecular Biology Morphology Motility Ovaries Phenotypes Polymerase chain reaction Postpartum period Protein interaction Protein-protein interactions Proteins Reverse transcription Rnf32 Sperm Sperm Motility - genetics Spermatids Spermatocytes Spermatogenesis Spermatogenesis - genetics Testes Testis Testis - metabolism Thermal cycling Ubiquitin-Protein Ligases - genetics Ubiquitin-Protein Ligases - metabolism |
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| Title | Rnf32 is not essential for spermatogenesis and male fertility in mice |
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