Essential Oil Bioactive Fibrous Membranes Prepared via Coaxial Electrospinning

A novel antimicrobial composite material was prepared by encapsulating orange essential oil (OEO) in zein prolamine (ZP) via the coaxial electrospinning (ES) technique. By manipulating process parameters, the morphological features of ZP/OEO fibers were modulated. Fine fibers with diameters ranging...

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Published inJournal of food science Vol. 82; no. 6; pp. 1412 - 1422
Main Authors Yao, Zhi‐Cheng, Chen, Si‐Cong, Ahmad, Zeeshan, Huang, Jie, Chang, Ming‐Wei, Li, Jing‐Song
Format Journal Article
LanguageEnglish
Published United States Wiley Subscription Services, Inc 01.06.2017
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Abstract A novel antimicrobial composite material was prepared by encapsulating orange essential oil (OEO) in zein prolamine (ZP) via the coaxial electrospinning (ES) technique. By manipulating process parameters, the morphological features of ZP/OEO fibers were modulated. Fine fibers with diameters ranging from 0.7 to 2.3 μm were obtained by regulating ZP solution concentration and process parameters during the ES process. Optimal loading capacity (LC) and encapsulation efficiency (EE) of OEO in fibrous ZP mats were determined to be 22.28% and 53.68%, respectively, and were achieved using a 35 w/v% ZP ES solution. The encapsulation of OEO was found to be reliant on ZP solution concentration (the enveloping medium). SEM analysis indicates the surface morphology of ZP/OEO electrospun fibers is dependent on ZP solution loading volume, with lower ZP concentrations yielding defective fibrous structures (for example, beaded and spindled‐string like morphologies). Furthermore, this loading volume also influences OEO LC, EE, mat water contact angle and oil retention. CCK‐8 assay and cell morphology assessment (HEK293T cells) indicate no significant change with electrospun ZP and ZP/OEO fibrous membranes over an 8 h period. Antimicrobial activity assessment using Escherichia coli, suggests composite nonwovens possess sterilization properties; elucidating potential application in active food packaging, food preservation and therefore sustainability. Practical Application Composite ZP/OEO fibrous membranes, fabricated using coaxial electrospinning technique, exhibit nontoxic in vitro behavior and antimicrobial potential. This indicates their potential application for bioactive food packaging improving sustainability.
AbstractList A novel antimicrobial composite material was prepared by encapsulating orange essential oil (OEO) in zein prolamine (ZP) via the coaxial electrospinning (ES) technique. By manipulating process parameters, the morphological features of ZP/OEO fibers were modulated. Fine fibers with diameters ranging from 0.7 to 2.3 μm were obtained by regulating ZP solution concentration and process parameters during the ES process. Optimal loading capacity (LC) and encapsulation efficiency (EE) of OEO in fibrous ZP mats were determined to be 22.28% and 53.68%, respectively, and were achieved using a 35 w/v% ZP ES solution. The encapsulation of OEO was found to be reliant on ZP solution concentration (the enveloping medium). SEM analysis indicates the surface morphology of ZP/OEO electrospun fibers is dependent on ZP solution loading volume, with lower ZP concentrations yielding defective fibrous structures (for example, beaded and spindled-string like morphologies). Furthermore, this loading volume also influences OEO LC, EE, mat water contact angle and oil retention. CCK-8 assay and cell morphology assessment (HEK293T cells) indicate no significant change with electrospun ZP and ZP/OEO fibrous membranes over an 8 h period. Antimicrobial activity assessment using Escherichia coli, suggests composite nonwovens possess sterilization properties; elucidating potential application in active food packaging, food preservation and therefore sustainability.A novel antimicrobial composite material was prepared by encapsulating orange essential oil (OEO) in zein prolamine (ZP) via the coaxial electrospinning (ES) technique. By manipulating process parameters, the morphological features of ZP/OEO fibers were modulated. Fine fibers with diameters ranging from 0.7 to 2.3 μm were obtained by regulating ZP solution concentration and process parameters during the ES process. Optimal loading capacity (LC) and encapsulation efficiency (EE) of OEO in fibrous ZP mats were determined to be 22.28% and 53.68%, respectively, and were achieved using a 35 w/v% ZP ES solution. The encapsulation of OEO was found to be reliant on ZP solution concentration (the enveloping medium). SEM analysis indicates the surface morphology of ZP/OEO electrospun fibers is dependent on ZP solution loading volume, with lower ZP concentrations yielding defective fibrous structures (for example, beaded and spindled-string like morphologies). Furthermore, this loading volume also influences OEO LC, EE, mat water contact angle and oil retention. CCK-8 assay and cell morphology assessment (HEK293T cells) indicate no significant change with electrospun ZP and ZP/OEO fibrous membranes over an 8 h period. Antimicrobial activity assessment using Escherichia coli, suggests composite nonwovens possess sterilization properties; elucidating potential application in active food packaging, food preservation and therefore sustainability.
A novel antimicrobial composite material was prepared by encapsulating orange essential oil (OEO) in zein prolamine (ZP) via the coaxial electrospinning (ES) technique. By manipulating process parameters, the morphological features of ZP/OEO fibers were modulated. Fine fibers with diameters ranging from 0.7 to 2.3 µm were obtained by regulating ZP solution concentration and process parameters during the ES process. Optimal loading capacity (LC) and encapsulation efficiency (EE) of OEO in fibrous ZP mats were determined to be 22.28% and 53.68%, respectively, and were achieved using a 35 w/v% ZP ES solution. The encapsulation of OEO was found to be reliant on ZP solution concentration (the enveloping medium). SEM analysis indicates the surface morphology of ZP/OEO electrospun fibers is dependent on ZP solution loading volume, with lower ZP concentrations yielding defective fibrous structures (for example, beaded and spindled-string like morphologies). Furthermore, this loading volume also influences OEO LC, EE, mat water contact angle and oil retention. CCK-8 assay and cell morphology assessment (HEK293T cells) indicate no significant change with electrospun ZP and ZP/OEO fibrous membranes over an 8 h period. Antimicrobial activity assessment using Escherichia coli, suggests composite nonwovens possess sterilization properties; elucidating potential application in active food packaging, food preservation and therefore sustainability. Practical Application Composite ZP/OEO fibrous membranes, fabricated using coaxial electrospinning technique, exhibit nontoxic in vitro behavior and antimicrobial potential. This indicates their potential application for bioactive food packaging improving sustainability.
A novel antimicrobial composite material was prepared by encapsulating orange essential oil (OEO) in zein prolamine (ZP) via the coaxial electrospinning (ES) technique. By manipulating process parameters, the morphological features of ZP/OEO fibers were modulated. Fine fibers with diameters ranging from 0.7 to 2.3 μm were obtained by regulating ZP solution concentration and process parameters during the ES process. Optimal loading capacity (LC) and encapsulation efficiency (EE) of OEO in fibrous ZP mats were determined to be 22.28% and 53.68%, respectively, and were achieved using a 35 w/v% ZP ES solution. The encapsulation of OEO was found to be reliant on ZP solution concentration (the enveloping medium). SEM analysis indicates the surface morphology of ZP/OEO electrospun fibers is dependent on ZP solution loading volume, with lower ZP concentrations yielding defective fibrous structures (for example, beaded and spindled-string like morphologies). Furthermore, this loading volume also influences OEO LC, EE, mat water contact angle and oil retention. CCK-8 assay and cell morphology assessment (HEK293T cells) indicate no significant change with electrospun ZP and ZP/OEO fibrous membranes over an 8 h period. Antimicrobial activity assessment using Escherichia coli, suggests composite nonwovens possess sterilization properties; elucidating potential application in active food packaging, food preservation and therefore sustainability.
A novel antimicrobial composite material was prepared by encapsulating orange essential oil (OEO) in zein prolamine (ZP) via the coaxial electrospinning (ES) technique. By manipulating process parameters, the morphological features of ZP/OEO fibers were modulated. Fine fibers with diameters ranging from 0.7 to 2.3 μm were obtained by regulating ZP solution concentration and process parameters during the ES process. Optimal loading capacity (LC) and encapsulation efficiency (EE) of OEO in fibrous ZP mats were determined to be 22.28% and 53.68%, respectively, and were achieved using a 35 w/v% ZP ES solution. The encapsulation of OEO was found to be reliant on ZP solution concentration (the enveloping medium). SEM analysis indicates the surface morphology of ZP/OEO electrospun fibers is dependent on ZP solution loading volume, with lower ZP concentrations yielding defective fibrous structures (for example, beaded and spindled‐string like morphologies). Furthermore, this loading volume also influences OEO LC, EE, mat water contact angle and oil retention. CCK‐8 assay and cell morphology assessment (HEK293T cells) indicate no significant change with electrospun ZP and ZP/OEO fibrous membranes over an 8 h period. Antimicrobial activity assessment using Escherichia coli , suggests composite nonwovens possess sterilization properties; elucidating potential application in active food packaging, food preservation and therefore sustainability. Composite ZP/OEO fibrous membranes, fabricated using coaxial electrospinning technique, exhibit nontoxic in vitro behavior and antimicrobial potential. This indicates their potential application for bioactive food packaging improving sustainability.
A novel antimicrobial composite material was prepared by encapsulating orange essential oil (OEO) in zein prolamine (ZP) via the coaxial electrospinning (ES) technique. By manipulating process parameters, the morphological features of ZP/OEO fibers were modulated. Fine fibers with diameters ranging from 0.7 to 2.3 μm were obtained by regulating ZP solution concentration and process parameters during the ES process. Optimal loading capacity (LC) and encapsulation efficiency (EE) of OEO in fibrous ZP mats were determined to be 22.28% and 53.68%, respectively, and were achieved using a 35 w/v% ZP ES solution. The encapsulation of OEO was found to be reliant on ZP solution concentration (the enveloping medium). SEM analysis indicates the surface morphology of ZP/OEO electrospun fibers is dependent on ZP solution loading volume, with lower ZP concentrations yielding defective fibrous structures (for example, beaded and spindled‐string like morphologies). Furthermore, this loading volume also influences OEO LC, EE, mat water contact angle and oil retention. CCK‐8 assay and cell morphology assessment (HEK293T cells) indicate no significant change with electrospun ZP and ZP/OEO fibrous membranes over an 8 h period. Antimicrobial activity assessment using Escherichia coli, suggests composite nonwovens possess sterilization properties; elucidating potential application in active food packaging, food preservation and therefore sustainability. Practical Application Composite ZP/OEO fibrous membranes, fabricated using coaxial electrospinning technique, exhibit nontoxic in vitro behavior and antimicrobial potential. This indicates their potential application for bioactive food packaging improving sustainability.
Author Li, Jing‐Song
Ahmad, Zeeshan
Huang, Jie
Chang, Ming‐Wei
Chen, Si‐Cong
Yao, Zhi‐Cheng
Author_xml – sequence: 1
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  surname: Yao
  fullname: Yao, Zhi‐Cheng
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  givenname: Si‐Cong
  surname: Chen
  fullname: Chen, Si‐Cong
  organization: Zhejiang Univ
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  givenname: Zeeshan
  surname: Ahmad
  fullname: Ahmad, Zeeshan
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  givenname: Jie
  surname: Huang
  fullname: Huang, Jie
  organization: Univ. College London
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  givenname: Ming‐Wei
  surname: Chang
  fullname: Chang, Ming‐Wei
  email: mwchang@zju.edu.cn
  organization: Zhejiang Univ
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  givenname: Jing‐Song
  surname: Li
  fullname: Li, Jing‐Song
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/28471056$$D View this record in MEDLINE/PubMed
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Issue 6
Keywords coaxial electrospinning
food packaging
orange essential oil
zein prolamine
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Publisher Wiley Subscription Services, Inc
Publisher_xml – name: Wiley Subscription Services, Inc
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SSID ssj0002236
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Snippet A novel antimicrobial composite material was prepared by encapsulating orange essential oil (OEO) in zein prolamine (ZP) via the coaxial electrospinning (ES)...
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StartPage 1412
SubjectTerms active food packaging
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
antimicrobial properties
Assaying
Bearing strength
Bioactive compounds
Biocompatibility
Biomedical materials
cell biology
Cell morphology
Cholecystokinin
coaxial electrospinning
Composite materials
Contact angle
Cytology
diameter
E coli
Efficiency
Electrospinning
Encapsulation
Escherichia coli
Escherichia coli - drug effects
Essential oils
Fibers
Food Packaging
Food preservation
HEK293 Cells
Humans
In vitro methods and tests
Mats
Mechanical Phenomena
Membranes
Membranes, Artificial
Morphology
oils
Oils & fats
Oils, Volatile - chemistry
Optimization
orange essential oil
phenylpropanolamine
Phenylpropanolamine - chemistry
Plant Oils - chemistry
Process parameters
scanning electron microscopy
Sterilization
Surgical implants
Sustainability
Zein
Zein - chemistry
zein prolamine
Title Essential Oil Bioactive Fibrous Membranes Prepared via Coaxial Electrospinning
URI https://onlinelibrary.wiley.com/doi/abs/10.1111%2F1750-3841.13723
https://www.ncbi.nlm.nih.gov/pubmed/28471056
https://www.proquest.com/docview/1906646347
https://www.proquest.com/docview/1895278552
https://www.proquest.com/docview/2000338688
Volume 82
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