Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains

• Published in: Revista do Instituto de Medicina Tropical de São Paulo Vol. 44; no. 5; pp. 239 - 244
• Main Authors: GUIMARÃES, Semíramis, SOGAYAR, Maria Inês T. Leme, FRANCO, Marcello
• Format: Journal Article
• Language: English
• Published: Brazil Instituto de Medicina Tropical de Sao Paulo 01.10.2002; Instituto de Medicina Tropical; Universidade de São Paulo (USP)
• Subjects: Adult; Animals; Antigens; Antigens, Protozoan - analysis; Axenic strains; Electrophoresis, Polyacrylamide Gel; Female; Giardia - chemistry; Giardia - immunology; Giardia - pathogenicity; Giardia duodenalis; Glycoproteins; Host-Parasite Interactions; Humans; Immunoblotting; Immunoglobulin G - analysis; Male; Membrane Proteins - analysis; Proteins; Protozoan Proteins - analysis; Rabbits; Trophozoites; TROPICAL MEDICINE; Proteins; Trophozoites; Antigens; Glycoproteins; Giardia duodenalis; Axenic strains

In the present study, we have analyzed by sodium docecyl sulphate - polyacrilamide gel electrophoresis (SDS-PAGE), immunoblotting and Concanavalin A blotting (Con A blotting) proteins of membrane fractions and soluble fractions obtained from Giardia duodenalis trophozoites of two axenic strains isol...