Optogenetic Tools for Manipulating Protein Subcellular Localization and Intracellular Signaling at Organelle Contact Sites

• Published in: Current protocols Vol. 1; no. 3; p. e71
• Main Author: Benedetti, Lorena
• Format: Journal Article
• Language: English
• Published: United States 01.03.2021
• Subjects: Animals; Optogenetics; Organelles - metabolism; Protein Multimerization; Protein Transport; Signal Transduction; protein reconstitution; light-dependent dimerization; protein-protein interaction; organelle contacts; optogenetics

Intracellular signaling processes are frequently based on direct interactions between proteins and organelles. A fundamental strategy to elucidate the physiological significance of such interactions is to utilize optical dimerization tools. These tools are based on the use of small proteins or domai...