A method of quantifying cell sorting yield in “real time”

• Published in: Cytometry. Part A Vol. 77A; no. 10; pp. 983 - 989
• Main Author: Osborne, Geoffrey W.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.10.2010
• Subjects: Animals; Cell Separation - methods; cell sorting; Cells, Cultured; CHO Cells; Cricetinae; Cricetulus; drop delay; Flow Cytometry - methods; Humans; Microspheres; Phagocytosis; yield

Cell sorting flow cytometers sort cells by applying electrical charges to a stream that forms liquid droplets containing the cells at a set time after sample interrogation. The correct time to apply these charges is determined based on calibration beads and automated technology. The central tenet of...