On the synonymy of Trophotylenchulus asoensis and T. okamotoi with T. arenarius, and intra-generic structure of Paratylenchus (Nematoda: Tylenchulidae)

• Published in: Journal of nematology Vol. 51; no. 1; pp. 1 - 14
• Main Authors: Mirbabaei, Hossein, Eskandari, Ali, Ghaderi, Reza, Karegar, Akbar
• Format: Journal Article
• Language: English
• Published: Sciendo 01.01.2019
• Subjects: 28 S rRNA; gracilacus; Identification; paratylenchus; Phylogeny; Taxonomy; trophotylenchulus; Tylenchulidae
• ISSN: 2640-396X; 2640-396X
• DOI: 10.21307/jofnem-2019-078

Two populations of the genus and 10 species of the genus from Iran were characterized based on morphometric, morphological and molecular characters. Our observations on the two populations of from Iran revealed that and have been distinguished from , on the basis of the features which cannot be longer considered as stable diagnostic characters. One of the populations shows a mixed combination of the characters of and ; it has morphometrics more similar to but shows affinities with in the tail terminus shape of females and second-stage juveniles (J2) and in having a reduced stylet in males. The other population fit well with ; it has females with generally bluntly rounded tails typical for , but sometimes with finely rounded tail termini, like those of or . The sequences of D2–D3 expansion segments of 28 S rRNA gene for the two populations are identical with each other, but only 4 bp (0.67%) difference with sequence deposited in the GenBank. Considering no stable difference allow separating species, synonymy of and with , which has already been proposed, is supported and confirmed here. All studied species with stylets longer than 40 µm, except , formed a basal cluster to and species of bearing stylets shorter than 40 µm; thus, validity of cannot be rejected using our data sets. However, the synonymy of was supported by the positioning of within representatives of in the inferred phylogenetic tree.