Real-time monitoring of polyacrylamide gel electrophoresis by the shadowgraph technique

• Published in: Journal of biochemistry (Tokyo) Vol. 173; no. 2; pp. 65 - 72
• Main Authors: Murakami, Satoshi, Adachi, Hiroaki, Matsumura, Hiroyoshi, Takano, Kazufumi, Inoue, Tsuyoshi, Mori, Yusuke
• Format: Journal Article
• Language: English
• Published: England 03.02.2023
• Subjects: Coloring Agents - analysis; Coloring Agents - chemistry; Electrophoresis, Polyacrylamide Gel; Proteins - chemistry; Sodium Dodecyl Sulfate; Staining and Labeling; SDS-PAGE; polyacrylamide gel electrophoresis; direct observation; shadowgraph; Abbreviations: CBB, Coomassie Brilliant Blue; PAGE, Polyacrylamide gel electrophoresis; SDS, Sodium dodecyl sulphate; protein analysis

Polyacrylamide gel electrophoresis (PAGE) with sodium dodecyl sulphate (SDS) and Coomassie brilliant blue (CBB) staining is widely used in protein research and requires time for electrophoresis, staining and destaining. Because the protein bands electrophoresed in the gel are invisible in most cases...