Kinetic analyses of two-steps oxidation from l-tyrosine to l-dopaquinone with tyrosinase by capillary electrophoresis/dynamic frontal analysis

• Published in: Analytical biochemistry Vol. 655; p. 114856
• Main Authors: Mine, Masanori, Mizuguchi, Hitoshi, Takayanagi, Toshio
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 15.10.2022
• Subjects: Capillary electrophoresis; Dynamic frontal analysis; enzyme kinetics; Kinetic analysis; L-dopa; oxidation; Two-steps oxidation; Tyrosinase; tyrosine; Kinetic analysis; Dynamic frontal analysis; Capillary electrophoresis; Two-steps oxidation; Tyrosinase

Tyrosinase catalyzes the oxidation of l-tyrosine in two stages to produce l-dopa and l-dopaquinone stepwise, and l-dopaquinone is subsequently converted to dopachrome. Most of the conventional analyses subjected only one-step reaction from l-tyrosine to l-dopa or from l-dopa to l-dopaquinone. In thi...