Regulation of sesquiterpenoid metabolism in recombinant and elicited Valeriana officinalis hairy roots

• Published in: Phytochemistry (Oxford) Vol. 125; pp. 43 - 53
• Main Authors: Ricigliano, Vincent, Kumar, Santosh, Kinison, Scott, Brooks, Christopher, Nybo, S. Eric, Chappell, Joe, Howarth, Dianella G.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2016
• Subjects: Acetates - pharmacology; Agrobacterium; Alkyl and Aryl Transferases; Anti-Anxiety Agents - chemistry; Anti-Anxiety Agents - isolation & purification; beta-caryophyllene; biosynthesis; Caprofoliaceae; complementary DNA; Cyclopentanes - pharmacology; cytochrome P-450; DNA, Complementary - chemistry; gene overexpression; genes; germacrene; Humans; Indenes - chemistry; medicinal properties; metabolites; methyl jasmonate; Molecular Structure; Oxylipins - pharmacology; Plant Roots - chemistry; Polyisoprenyl Phosphates - chemistry; roots; Secondary metabolism regulation; sequence homology; Sesquiterpenes - analysis; Sesquiterpenes - chemistry; Sesquiterpenes - isolation & purification; Sesquiterpenoid; Valerian - chemistry; Valerian - genetics; Valerian root; Valeriana officinalis; Caprofoliaceae; RJZWGDPBGWGJNU-MTXIQXFFSA-N; NPNUFJAVOOONJE-GFUGXAQUSA-N; Agrobacterium; MZZFDMZYIBWOOA-KWCYVHTRSA-N; Valeriana officinalis; FEBNTWHYQKGEIQ-SUKRRCERSA-N; Secondary metabolism regulation; Valerian root; Sesquiterpenoid

Binary vector co-transformation of Valeriana officinalis hairy roots and pathway-level metabolic analyses facilitated identification of key steps in the biosynthesis of pharmacologically active sesquiterpenoids valerenic acid, valerenal, valerenadiene and β-caryophyllene and their potential regulati...