Hairy root transformation of Brassica rapa with bacterial halogenase genes and regeneration to adult plants to modify production of indolic compounds

• Published in: Phytochemistry (Oxford) Vol. 175; p. 112371
• Main Authors: Neumann, Madeleine, Prahl, Swantje, Caputi, Lorenzo, Hill, Lionel, Kular, Baldeep, Walter, Antje, Patallo, Eugenio P., Milbredt, Daniela, Aires, Alfredo, Schöpe, Maria, O'Connor, Sarah, van Pée, Karl-Heinz, Ludwig-Müller, Jutta
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2020
• Subjects: Agrobacterium rhizogenes; Brassica; Brassica rapa; Brassica rapa subsp. pekinensis; Brassicaceae; Chinese cabbage; Cl-IAN; Cl-trp; enzyme activity; enzymes; Flavin-dependent tryptophan halogenases; genes; Glucosinolates; Hairy root cultures; halogens; indole acetic acid; Indoles; mature plants; metabolites; phenotype; Plant regeneration; Plant Roots; Plants, Genetically Modified; roots; seeds; temperature; transgenic plants; tryptophan; Cl-trp; Chinese cabbage; Hairy root cultures; Brassica rapa subsp. pekinensis; Agrobacterium rhizogenes; Brassicaceae; Plant regeneration; Cl-IAN; Flavin-dependent tryptophan halogenases

During the last years halogenated compounds have drawn a lot of attention. Metabolites with one or more halogen atoms are often more active than their non-halogenated derivatives like indole-3-acetic acid (IAA) and 4-Cl-IAA. Within this work, bacterial flavin-dependent tryptophan halogenase genes we...