Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells

• Published in: Metabolic engineering Vol. 66; pp. 268 - 282
• Main Authors: Marx, Nicolas, Dhiman, Heena, Schmieder, Valerie, Freire, Catarina Martins, Nguyen, Ly Ngoc, Klanert, Gerald, Borth, Nicole
• Format: Journal Article
• Language: English
• Published: Belgium Elsevier Inc 01.07.2021
• Subjects: CHO; CMV promoter; CRISPR/dCas9; DNA methylation; Epigenetic editing; Histone modifications; UHRF1; CRISPR/dCas9; H3K9me3; CHO; FACS; CMV promoter; ChIP; BFP; bp; gRNA; DNMT; FUT8; hCMV-MIE; HDR; LSD1; DNA methylation; KD; cDNA; sfGFP; CpG; ADCC; gDNA; GOI; PBS; CD; CRISPR; ST6GAL1; Epigenetic editing; H3K4me3; H3K27ac; CAG; dCas9; TET1; Histone modifications; KRAB; LCA; MSR; InDel; TSS; GlcNac; Cas9; NHEJ; MCS; BS-DNA; GAPDH; PAM; H3K27me3; HDAC

With the emergence of new CRISPR/dCas9 tools that enable site specific modulation of DNA methylation and histone modifications, more detailed investigations of the contribution of epigenetic regulation to the precise phenotype of cells in culture, including recombinant production subclones, is now p...