Enhanced L-theanine production through semi-rational design of γ-glutamylmethylamide synthetase from Methylovorus mays - Fan, Chao; Qi, Jiakun; Cong, Yunhan; Zhang, Chunzhi (Enzyme and microbial technology, 2024)
Keywords: Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; biosynthesis; Carbon-Nitrogen Ligases; catalytic activity; directed evolution; Directed Molecular Evolution; Disulfide bridge; disulfides; enzyme activity; Enzyme Stability; enzymes; Glutamates - biosynthesis; Glutamates - metabolism; half life; Kinetics; L-theanine; Methylovorus mays; Peptide Synthases - chemistry; Peptide Synthases - genetics; Peptide Synthases - metabolism; Semi-rational design; Site-directed mutations; technology; Temperature; Thermostability; γ-glutamylmethylamide synthetase; Thermostability; Site-directed mutations; L-theanine; γ-glutamylmethylamide synthetase; Disulfide bridge; Semi-rational design