Isolation and culture of adult murine cardiac atrial and ventricular fibroblasts and myofibroblasts

• Published in: Methods (San Diego, Calif.) Vol. 203; pp. 187 - 195
• Main Authors: Sahadevan, Pramod, Allen, Bruce G.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2022
• Subjects: Atria; Fibroblast activation; Fibroblast isolation; Heart; Ventricle; Heart; Fibroblast activation; Ventricle; Atria; Fibroblast isolation

•Fibroblasts can be isolated from mouse or rat cardiac ventricles or atria.•Yield sufficient to study the effects of pharmacological or molecular interventions.•High elastic modulus (107 kPa) polystyrene substrates activate fibroblasts in culture.•Low elastic modulus (e.g., 8 kPa) ‘compliant’ substr...