A microassay for measurement of Fc function of human immunoglobulin preparations by using tetanus toxoid as antigen
In order to minimize possible adverse reactions, the functional integrity of proteins in products derived from human plasma has to be unaffected by methods of preparation and storage conditions. Numerous biologically relevant functions of IgG, a major component of immunoglobulin for intravenous use...
Saved in:
Published in | Biologicals Vol. 32; no. 2; pp. 78 - 83 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.06.2004
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | In order to minimize possible adverse reactions, the functional integrity of proteins in products derived from human plasma has to be unaffected by methods of preparation and storage conditions. Numerous biologically relevant functions of IgG, a major component of immunoglobulin for intravenous use preparations (IVIG), rely on the integrity of Fc fragments. Manufacturers are obliged to prove that Fc-mediated functions are maintained in IVIG preparations. The European Pharmacopoeia's monograph proposes a Rubella antigen-based test for Fc function of immunoglobulins. We present a modification of the proposed method achieved by using more convenient and readily available tetanus toxoid as an alternative antigen target and by adapting the procedure for the use on microtitre plates, thus greatly enhancing its feasibility and sample throughput. The test conditions were optimized so that batch-to-batch variability in tetanus antibody content did not influence the result. The precision of the test was within ± 5%. By using this test, we compared Fc functionality of 9 commercial IVIG-7S preparations, which were prepared by using different virus inactivation/removal approaches. No significant differences between them have been found. |
---|---|
AbstractList | In order to minimize possible adverse reactions, the functional integrity of proteins in products derived from human plasma has to be unaffected by methods of preparation and storage conditions. Numerous biologically relevant functions of IgG, a major component of immunoglobulin for intravenous use preparations (IVIG), rely on the integrity of Fc fragments. Manufacturers are obliged to prove that Fc-mediated functions are maintained in IVIG preparations. The European Pharmacopoeia's monograph proposes a Rubella antigen-based test for Fc function of immunoglobulins. We present a modification of the proposed method achieved by using more convenient and readily available tetanus toxoid as an alternative antigen target and by adapting the procedure for the use on microtitre plates, thus greatly enhancing its feasibility and sample throughput. The test conditions were optimized so that batch-to-batch variability in tetanus antibody content did not influence the result. The precision of the test was within +/- 5%. By using this test, we compared Fc functionality of 9 commercial IVIG-7S preparations, which were prepared by using different virus inactivation/removal approaches. No significant differences between them have been found. In order to minimize possible adverse reactions, the functional integrity of proteins in products derived from human plasma has to be unaffected by methods of preparation and storage conditions. Numerous biologically relevant functions of IgG, a major component of immunoglobulin for intravenous use preparations (IVIG), rely on the integrity of Fc fragments. Manufacturers are obliged to prove that Fc-mediated functions are maintained in IVIG preparations. The European Pharmacopoeia's monograph proposes a Rubella antigen-based test for Fc function of immunoglobulins. We present a modification of the proposed method achieved by using more convenient and readily available tetanus toxoid as an alternative antigen target and by adapting the procedure for the use on microtitre plates, thus greatly enhancing its feasibility and sample throughput. The test conditions were optimized so that batch-to-batch variability in tetanus antibody content did not influence the result. The precision of the test was within ± 5%. By using this test, we compared Fc functionality of 9 commercial IVIG-7S preparations, which were prepared by using different virus inactivation/removal approaches. No significant differences between them have been found. |
Author | Simic, Mirjana Benko, Bojan Trescec, Anda Vrdoljak, Anto |
Author_xml | – sequence: 1 givenname: Anto surname: Vrdoljak fullname: Vrdoljak, Anto email: avrdoljak@imz.hr – sequence: 2 givenname: Anda surname: Trescec fullname: Trescec, Anda – sequence: 3 givenname: Bojan surname: Benko fullname: Benko, Bojan – sequence: 4 givenname: Mirjana surname: Simic fullname: Simic, Mirjana |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/15454185$$D View this record in MEDLINE/PubMed |
BookMark | eNqNkcuKFDEUhoOMOBd9BYkbd1UmqaQuy6FxdGDAja5DKjlp01QlbS5iv_2k6AbdOatzDnzn9v-36MoHDwh9oKSlhPafDu3swhL2TqsltYwQ3pKuJYS-QjeUTKIZO0autpyLhhLRX6PblA4VoHzgb9A1FVxwOooblO7x6nQMKiV1wjZEvIJKJcIKPuNg8YPGtnidXfBb-bOsymO3rsWH_RLmsjiPjxGOKqqNSXg-4ZKc3-MMWfmScA5_gjNYJax8dnvwb9FrWw-Hd5d4h348fP6--9o8ffvyuLt_ajQnPDejmsjMBqFmPTA2MmrmHrrJdCMw0o_1T2tnA52hcy-oNcZwwzk3bLIWgNjuDn08zz3G8KtAynJ1ScOyKA-hJNn3E-sZH_8L0mEQ9RBewekMVsVSimDlMbpVxZOkRG7WyIP8xxq5WSNJJ6vytff9ZUmZVzB_Oy9eVGB3BqBq8ttBlEk78BqMi6CzNMG9YM0zCjmqZw |
CitedBy_id | crossref_primary_10_1111_j_1423_0410_2008_01125_x crossref_primary_10_1111_j_1423_0410_2009_01226_x crossref_primary_10_1155_2012_134081 crossref_primary_10_1016_j_jpba_2018_01_021 |
Cites_doi | 10.1046/j.1365-3156.2001.00697.x 10.1007/BF01958951 10.1093/infdis/151.2.217 10.1111/j.1423-0410.1994.tb01270.x 10.1002/eji.1830140918 10.1046/j.1423-0410.2000.7830185.x 10.1053/ajkd.2001.29223 10.1023/A:1025375932257 10.1016/0161-5890(86)90060-X 10.1128/CDLI.1.2.172-175.1994 10.1016/0022-1759(84)90209-6 10.4314/eamj.v79i2.8904 |
ContentType | Journal Article |
Copyright | 2004 The International Association for Biologicals |
Copyright_xml | – notice: 2004 The International Association for Biologicals |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QO 8FD FR3 P64 7X8 |
DOI | 10.1016/j.biologicals.2004.03.001 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Biotechnology Research Abstracts Technology Research Database Engineering Research Database Biotechnology and BioEngineering Abstracts MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Engineering Research Database Biotechnology Research Abstracts Technology Research Database Biotechnology and BioEngineering Abstracts MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic Engineering Research Database MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
EISSN | 1095-8320 |
EndPage | 83 |
ExternalDocumentID | 10_1016_j_biologicals_2004_03_001 15454185 S1045105604000168 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- --K --M .~1 0R~ 1B1 1RT 1~. 1~5 23N 4.4 457 4G. 53G 5GY 5VS 6J9 7-5 71M 8P~ 9JM AAAJQ AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARKO AAXUO ABBQC ABFNM ABFRF ABGSF ABJNI ABLVK ABMAC ABMZM ABUDA ABXDB ABYKQ ACDAQ ACGFO ACGFS ACRLP ADBBV ADEZE ADFGL ADMUD ADUVX AEBSH AEFWE AEHWI AEKER AENEX AFKWA AFTJW AFXIZ AGEKW AGHFR AGRDE AGUBO AGYEJ AHHHB AIEXJ AIKHN AITUG AJBFU AJOXV AJRQY ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC BNPGV CAG CJTIS COF CS3 DM4 DOVZS DU5 EBS EFBJH EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HEJ HMG HMK HMO HVGLF HZ~ IHE J1W KOM LCYCR LG5 LUGTX LZ5 M29 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SAE SDF SDG SDP SES SEW SIN SPCBC SSH SSI SSU SSZ T5K UNMZH WUQ XPP ZMT ~G- ~KM AAHBH AAXKI AFJKZ AKRWK CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QO 8FD FR3 P64 7X8 |
ID | FETCH-LOGICAL-c404t-8a90b275abc722821db6e39d38e2068095ffbde3d1b651fddd4d444d29ffee0f3 |
IEDL.DBID | .~1 |
ISSN | 1045-1056 |
IngestDate | Sat Aug 17 03:24:24 EDT 2024 Fri Aug 16 23:24:02 EDT 2024 Thu Sep 26 18:25:05 EDT 2024 Sat Sep 28 07:45:10 EDT 2024 Fri Feb 23 02:34:23 EST 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 2 |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c404t-8a90b275abc722821db6e39d38e2068095ffbde3d1b651fddd4d444d29ffee0f3 |
Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
PMID | 15454185 |
PQID | 17752754 |
PQPubID | 23462 |
PageCount | 6 |
ParticipantIDs | proquest_miscellaneous_66926248 proquest_miscellaneous_17752754 crossref_primary_10_1016_j_biologicals_2004_03_001 pubmed_primary_15454185 elsevier_sciencedirect_doi_10_1016_j_biologicals_2004_03_001 |
PublicationCentury | 2000 |
PublicationDate | 2004-06-01 |
PublicationDateYYYYMMDD | 2004-06-01 |
PublicationDate_xml | – month: 06 year: 2004 text: 2004-06-01 day: 01 |
PublicationDecade | 2000 |
PublicationPlace | England |
PublicationPlace_xml | – name: England |
PublicationTitle | Biologicals |
PublicationTitleAlternate | Biologicals |
PublicationYear | 2004 |
Publisher | Elsevier Ltd |
Publisher_xml | – name: Elsevier Ltd |
References | Moli, Nisini, Fattorossi, Matricardi, D'Amelio (BIB4) 1989; 29 Seppälä, Routonen, Sarnesto, Mattila, Mäkelä (BIB14) 1984; 14 Lagergard, Thiringer, Wassen, Schneerson, Trollfors (BIB17) 1992; 151 Ph Eur. 2002:2.7.9. Kotitschke (BIB10) 1993; 20 Aboud, Matre, Lyamuya, Kristoffersen (BIB15) 2001; 6 Gronski, Kanzy, Seiler (BIB9) 1991; 3 Bird, Lowe, Stokes, Bird, Ling, Jefferis (BIB13) 1984; 71 Swartz, Saliou, Catznelson, Blondeau, Gil, Peled (BIB18) 2003; 18 Law, Painter (BIB7) 1986; 23 (BIB3) 1983 Ramasamy, Tran, Charnock, Farrugia (BIB11) 2000; 78 Yang, Ngo, Yeh, Uemura (BIB12) 1994; 67 (BIB2) 1980 Aboud, Lyamuya, Kristoffersen, Matre (BIB19) 2002; 79 Givner, Edwards, Anderson, Baker (BIB6) 1985; 151 Morell (BIB1) 1985 Tono-Oka, Matsumoto, Imai, Matsumoto (BIB5) 1985; 39 Kruger, Muller-Steinhardt, Kirchner, Kreft (BIB20) 2001; 38 Semba, Muhilal, Scott, Natadisastra, West, Sommer (BIB16) 1994; 1 Ramasamy (10.1016/j.biologicals.2004.03.001_BIB11) 2000; 78 Tono-Oka (10.1016/j.biologicals.2004.03.001_BIB5) 1985; 39 Swartz (10.1016/j.biologicals.2004.03.001_BIB18) 2003; 18 Gronski (10.1016/j.biologicals.2004.03.001_BIB9) 1991; 3 Morell (10.1016/j.biologicals.2004.03.001_BIB1) 1985 Seppälä (10.1016/j.biologicals.2004.03.001_BIB14) 1984; 14 (10.1016/j.biologicals.2004.03.001_BIB2) 1980 Kotitschke (10.1016/j.biologicals.2004.03.001_BIB10) 1993; 20 Semba (10.1016/j.biologicals.2004.03.001_BIB16) 1994; 1 Aboud (10.1016/j.biologicals.2004.03.001_BIB15) 2001; 6 Yang (10.1016/j.biologicals.2004.03.001_BIB12) 1994; 67 Aboud (10.1016/j.biologicals.2004.03.001_BIB19) 2002; 79 Kruger (10.1016/j.biologicals.2004.03.001_BIB20) 2001; 38 10.1016/j.biologicals.2004.03.001_BIB8 Givner (10.1016/j.biologicals.2004.03.001_BIB6) 1985; 151 Lagergard (10.1016/j.biologicals.2004.03.001_BIB17) 1992; 151 (10.1016/j.biologicals.2004.03.001_BIB3) 1983 Moli (10.1016/j.biologicals.2004.03.001_BIB4) 1989; 29 Law (10.1016/j.biologicals.2004.03.001_BIB7) 1986; 23 Bird (10.1016/j.biologicals.2004.03.001_BIB13) 1984; 71 |
References_xml | – volume: 151 start-page: 98 year: 1992 end-page: 102 ident: BIB17 article-title: Isotype composition of antibodies to streptococcus group B type III polysaccharide and to tetanus toxoid in maternal, cord blood sera and in breast milk publication-title: Eur J Pediatr contributor: fullname: Trollfors – volume: 20 start-page: 93 year: 1993 end-page: 95 ident: BIB10 article-title: Assessment of the Fc function in human immunoglobulins for intravenous administration publication-title: Infusionsther Transfusionsmed contributor: fullname: Kotitschke – volume: 71 start-page: 97 year: 1984 end-page: 105 ident: BIB13 article-title: The separation of human serum IgG into subclass fractions by immunoaffinity chromatography and assessment of specific antibody activity publication-title: J Immunol Methods contributor: fullname: Jefferis – volume: 18 start-page: 827 year: 2003 end-page: 833 ident: BIB18 article-title: Immune response to a diphtheria and tetanus toxoid administration in a three-dose diphtheria tetanus whole-cell pertussis/enhanced inactivated poliovirus vaccination schedule: a 7-year follow up publication-title: Eur J Epidemiol contributor: fullname: Peled – year: 1983 ident: BIB3 publication-title: Separation of plasma proteins – volume: 39 start-page: 477 year: 1985 end-page: 481 ident: BIB5 article-title: Effect of gammaglobulin preparations on phagocytic function of whole blood publication-title: Biomed Pharmacother contributor: fullname: Matsumoto – volume: 67 start-page: 337 year: 1994 end-page: 344 ident: BIB12 article-title: Antibody Fc functional activity of intravenous immunoglobulin preparations treated with solvent-detergent for virus inactivation publication-title: Vox Sang contributor: fullname: Uemura – volume: 151 start-page: 217 year: 1985 end-page: 220 ident: BIB6 article-title: Immune globulin for intravenous use: enhancement of in vitro opsonophagocytic activity of neonatal serum publication-title: J Infect Dis contributor: fullname: Baker – volume: 3 start-page: 287 year: 1991 end-page: 292 ident: BIB9 article-title: Measurement of Fc function on human immunoglobulin preparations publication-title: Pharmeuropa contributor: fullname: Seiler – volume: 14 start-page: 868 year: 1984 end-page: 875 ident: BIB14 article-title: The percentages of six immunoglobulin isotypes in human antibodies to tetanus toxoid: standardization of isotype-specific second antibodies in solid-phase assay publication-title: Eur J Immunol contributor: fullname: Mäkelä – volume: 23 start-page: 331 year: 1986 end-page: 338 ident: BIB7 article-title: An examination of the structural and biological properties of three intravenous immunoglobulin preparations publication-title: Mol Immunol contributor: fullname: Painter – volume: 1 start-page: 172 year: 1994 end-page: 175 ident: BIB16 article-title: Effect of vitamin A supplementation on immunoglobulin G subclass responses to tetanus toxoid in children publication-title: Clin Diagn Lab Immunol contributor: fullname: Sommer – start-page: 47 year: 1985 end-page: 59 ident: BIB1 article-title: The perfect immunoglobulin replacement therapy publication-title: Intravenous immunoglobulins contributor: fullname: Morell – volume: 29 start-page: 79 year: 1989 end-page: 84 ident: BIB4 article-title: Intravenous immunoglobulin preparations. A comparative in vitro study of Fc mediated functions publication-title: J Clin Lab Immunol contributor: fullname: D'Amelio – volume: 6 start-page: 119 year: 2001 end-page: 125 ident: BIB15 article-title: Antibodies to tetanus toxoid in women of childbearing age in Dar es Salaam and Bagamoyo, Tanzania publication-title: Trop Med Int Health contributor: fullname: Kristoffersen – volume: 79 start-page: 73 year: 2002 end-page: 76 ident: BIB19 article-title: Immunity to tetanus in male adults in Dar es Salaam, Tanzania publication-title: East Afr Med J contributor: fullname: Matre – volume: 78 start-page: 185 year: 2000 end-page: 193 ident: BIB11 article-title: Flow-cytometric method for the quantitation of the Fc function of intravenous immunoglobulin preparations publication-title: Vox Sang contributor: fullname: Farrugia – year: 1980 ident: BIB2 publication-title: Methods of plasma protein fractionation – volume: 38 start-page: 1264 year: 2001 end-page: 1270 ident: BIB20 article-title: A 5-year follow-up on antibody response after diphtheria and tetanus vaccination in hemodialysis patients publication-title: Am J Kidney Dis contributor: fullname: Kreft – volume: 6 start-page: 119 issue: 2 year: 2001 ident: 10.1016/j.biologicals.2004.03.001_BIB15 article-title: Antibodies to tetanus toxoid in women of childbearing age in Dar es Salaam and Bagamoyo, Tanzania publication-title: Trop Med Int Health doi: 10.1046/j.1365-3156.2001.00697.x contributor: fullname: Aboud – ident: 10.1016/j.biologicals.2004.03.001_BIB8 – volume: 151 start-page: 98 issue: 2 year: 1992 ident: 10.1016/j.biologicals.2004.03.001_BIB17 article-title: Isotype composition of antibodies to streptococcus group B type III polysaccharide and to tetanus toxoid in maternal, cord blood sera and in breast milk publication-title: Eur J Pediatr doi: 10.1007/BF01958951 contributor: fullname: Lagergard – volume: 20 start-page: 93 year: 1993 ident: 10.1016/j.biologicals.2004.03.001_BIB10 article-title: Assessment of the Fc function in human immunoglobulins for intravenous administration publication-title: Infusionsther Transfusionsmed contributor: fullname: Kotitschke – volume: 151 start-page: 217 year: 1985 ident: 10.1016/j.biologicals.2004.03.001_BIB6 article-title: Immune globulin for intravenous use: enhancement of in vitro opsonophagocytic activity of neonatal serum publication-title: J Infect Dis doi: 10.1093/infdis/151.2.217 contributor: fullname: Givner – volume: 67 start-page: 337 issue: 4 year: 1994 ident: 10.1016/j.biologicals.2004.03.001_BIB12 article-title: Antibody Fc functional activity of intravenous immunoglobulin preparations treated with solvent-detergent for virus inactivation publication-title: Vox Sang doi: 10.1111/j.1423-0410.1994.tb01270.x contributor: fullname: Yang – start-page: 47 year: 1985 ident: 10.1016/j.biologicals.2004.03.001_BIB1 article-title: The perfect immunoglobulin replacement therapy contributor: fullname: Morell – volume: 29 start-page: 79 year: 1989 ident: 10.1016/j.biologicals.2004.03.001_BIB4 article-title: Intravenous immunoglobulin preparations. A comparative in vitro study of Fc mediated functions publication-title: J Clin Lab Immunol contributor: fullname: Moli – year: 1980 ident: 10.1016/j.biologicals.2004.03.001_BIB2 – volume: 14 start-page: 868 year: 1984 ident: 10.1016/j.biologicals.2004.03.001_BIB14 article-title: The percentages of six immunoglobulin isotypes in human antibodies to tetanus toxoid: standardization of isotype-specific second antibodies in solid-phase assay publication-title: Eur J Immunol doi: 10.1002/eji.1830140918 contributor: fullname: Seppälä – volume: 78 start-page: 185 year: 2000 ident: 10.1016/j.biologicals.2004.03.001_BIB11 article-title: Flow-cytometric method for the quantitation of the Fc function of intravenous immunoglobulin preparations publication-title: Vox Sang doi: 10.1046/j.1423-0410.2000.7830185.x contributor: fullname: Ramasamy – volume: 38 start-page: 1264 issue: 6 year: 2001 ident: 10.1016/j.biologicals.2004.03.001_BIB20 article-title: A 5-year follow-up on antibody response after diphtheria and tetanus vaccination in hemodialysis patients publication-title: Am J Kidney Dis doi: 10.1053/ajkd.2001.29223 contributor: fullname: Kruger – volume: 18 start-page: 827 issue: 8 year: 2003 ident: 10.1016/j.biologicals.2004.03.001_BIB18 article-title: Immune response to a diphtheria and tetanus toxoid administration in a three-dose diphtheria tetanus whole-cell pertussis/enhanced inactivated poliovirus vaccination schedule: a 7-year follow up publication-title: Eur J Epidemiol doi: 10.1023/A:1025375932257 contributor: fullname: Swartz – volume: 3 start-page: 287 year: 1991 ident: 10.1016/j.biologicals.2004.03.001_BIB9 article-title: Measurement of Fc function on human immunoglobulin preparations publication-title: Pharmeuropa contributor: fullname: Gronski – volume: 39 start-page: 477 year: 1985 ident: 10.1016/j.biologicals.2004.03.001_BIB5 article-title: Effect of gammaglobulin preparations on phagocytic function of whole blood publication-title: Biomed Pharmacother contributor: fullname: Tono-Oka – volume: 23 start-page: 331 year: 1986 ident: 10.1016/j.biologicals.2004.03.001_BIB7 article-title: An examination of the structural and biological properties of three intravenous immunoglobulin preparations publication-title: Mol Immunol doi: 10.1016/0161-5890(86)90060-X contributor: fullname: Law – volume: 1 start-page: 172 issue: 2 year: 1994 ident: 10.1016/j.biologicals.2004.03.001_BIB16 article-title: Effect of vitamin A supplementation on immunoglobulin G subclass responses to tetanus toxoid in children publication-title: Clin Diagn Lab Immunol doi: 10.1128/CDLI.1.2.172-175.1994 contributor: fullname: Semba – volume: 71 start-page: 97 year: 1984 ident: 10.1016/j.biologicals.2004.03.001_BIB13 article-title: The separation of human serum IgG into subclass fractions by immunoaffinity chromatography and assessment of specific antibody activity publication-title: J Immunol Methods doi: 10.1016/0022-1759(84)90209-6 contributor: fullname: Bird – year: 1983 ident: 10.1016/j.biologicals.2004.03.001_BIB3 – volume: 79 start-page: 73 issue: 2 year: 2002 ident: 10.1016/j.biologicals.2004.03.001_BIB19 article-title: Immunity to tetanus in male adults in Dar es Salaam, Tanzania publication-title: East Afr Med J doi: 10.4314/eamj.v79i2.8904 contributor: fullname: Aboud |
SSID | ssj0011474 |
Score | 1.690222 |
Snippet | In order to minimize possible adverse reactions, the functional integrity of proteins in products derived from human plasma has to be unaffected by methods of... |
SourceID | proquest crossref pubmed elsevier |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 78 |
SubjectTerms | Antibody Formation - immunology Antigens, Viral - chemistry Buffers Chemistry - methods Erythrocytes Hemolysis Humans Hydrogen-Ion Concentration Immunoglobulins - chemistry Immunoglobulins - immunology Immunoglobulins, Intravenous - chemistry Reproducibility of Results Research Design Rubella - immunology Rubella - prevention & control Rubella virus - chemistry Rubella virus - immunology Tetanus Toxoid - chemistry Tetanus Toxoid - immunology Time Factors |
Title | A microassay for measurement of Fc function of human immunoglobulin preparations by using tetanus toxoid as antigen |
URI | https://dx.doi.org/10.1016/j.biologicals.2004.03.001 https://www.ncbi.nlm.nih.gov/pubmed/15454185 https://search.proquest.com/docview/17752754 https://search.proquest.com/docview/66926248 |
Volume | 32 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Na9tAEB2MS0ouoUnz4SZxttCran2s5BXkYkyM2ySmNDH4JiTvbHAglollqC_57ZnRSnV6MBRyEhIrWM2MZp5Wb98AfEM3VYgondj10ZEaY4dQvaavVqWMij1tSsWb21E0HMufk3DSgH69F4ZplVXutzm9zNbVlU5lzc5iNuvceSyNQvWbw5CAC2_4lVSMKKa_v_yleRDcL5WYebDDoz_C1w3HywodsTVK5W5p9U69bTVqGwYta9HgE-xVIFL07Dz3oYHzA9ixbSXXn2HZE0_MsyNcnK4FoVLxtFkJFLkRg6ngesY-4dOyT5-Y8U6RnAVCmJwuFs9oZcEpLEW2FkyQfxAFEphcLUWR_8lnWqRLQZ5hQc9DGA-u7vtDp2qu4EylKwtHpbGb-d0wzaZdn767PJ1FGMQ6UOhzP444NCbTGGgvi0LPaK2lllJqPzYG0TXBETTn-RxPQCiZsSydj-QOGhHFOjSUCgyVflSp0S3wa3MmC6uhkdTkssfkjQ-4J6ZM3ICJdi24rA2f_BMQCeX6_7n9onZWQi8M_wVJ55ivlonX7Yb03HL7iChiEUWpWnBsvbyZNeFNVvv58r7JncKuJQDxYs4ZNIvnFZ4Ttimydhm8bfjQ6_---cXHH9fD0SvPcf4M |
link.rule.ids | 315,786,790,4521,24144,27957,27958,45620,45714 |
linkProvider | Elsevier |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3fa9swED66lG59KV33K9vaqrBXE8uWbRn6EkpD-isva6Fvwo5OI4PGoXFg-e93Z9lL9xAY7NFGAll3vvskffoO4BuGhUZEFeRhhIGymAeE6i2tWrV2OpfWNYo3d5N0_KCuH5PHHbjo7sIwrbKN_T6mN9G6fTNoZ3OwmM0G3yVLo1D-Zjck4KJfwa5KMql6sDu8uhlP_hwmSNWIMXP7gDu8hrMNzctrHfGENOLdykueym1pahsMbdLR6BAOWhwphn6ob2EH50ew5ytLrt_BciiemGpH0LhYCwKm4mmzGSgqJ0ZTwSmNzcKPTak-MePLIhVrhDA_XSye0SuDk2eKci2YI_9D1Eh4crUUdfWrmllRLAUZhzU938PD6PL-Yhy09RWCqQpVHegiD8soS4pymkW09JK2TDHObawx4pIceeJcaTG2skwT6ay1yiqlbJQ7hxi6-AP05tUcP4HQqmRlugjJItQizW3iKBo4yv6oC2f7EHXTaRZeRsN0_LKf5oUNuCymMmHMXLs-nHcTb_7yCUPh_l-6n3bGMvTP8EFIMcdqtTQyyxL6brW9RZqyjqLSffjorbwZNUFOFvz5_H-DO4U34_u7W3N7Nbn5AvueD8R7O1-hVz-v8JigTl2etK78G47v_y0 |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+microassay+for+measurement+of+Fc+function+of+human+immunoglobulin+preparations+by+using+tetanus+toxoid+as+antigen&rft.jtitle=Biologicals&rft.au=Vrdoljak%2C+Anto&rft.au=Trescec%2C+Anda&rft.au=Benko%2C+Bojan&rft.au=Simic%2C+Mirjana&rft.date=2004-06-01&rft.issn=1045-1056&rft.volume=32&rft.issue=2&rft.spage=78&rft.epage=83&rft_id=info:doi/10.1016%2Fj.biologicals.2004.03.001&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_j_biologicals_2004_03_001 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1045-1056&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1045-1056&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1045-1056&client=summon |