Molecular Mechanisms Underlying the Time-dependent Autophagy and Apoptosis Induced by Nutrient Depletion in Multiple Myeloma:a Pilot Study

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 32; no. 1; pp. 1 - 8
• Main Author: 刘媛 陈燕 文璐 崔国惠
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.02.2012
• Subjects: Apoptosis; Autophagy; c-Jun amino-terminal kinase; Cell Line, Tumor; Cyclin-Dependent Kinase Inhibitor p21 - metabolism; Humans; Intracellular Signaling Peptides and Proteins; JNK3 protein; MAP Kinase Kinase 4 - metabolism; MAP Kinase Signaling System; Medicine; Medicine & Public Health; Molecular modelling; multiple; Multiple myeloma; Multiple Myeloma - complications; Multiple Myeloma - metabolism; Multiple Myeloma - pathology; myeloma;RPMI;autophagy;apoptosis;Deptor;Raf-/JNK/p/p;NFκB; NF-kappa B p50 Subunit - metabolism; Nutrients; Nutrition Disorders - etiology; Nutrition Disorders - metabolism; p53 protein; Phagocytosis; Pilot Projects; Polymerase chain reaction; Proto-Oncogene Proteins c-raf - metabolism; Signal transduction; Time Factors; TOR Serine-Threonine Kinases - metabolism; Transcription; Tumor cell lines; Tumor Suppressor Protein p53 - metabolism; multiple myeloma; autophagy; Deptor; NFκB1; apoptosis; Raf-1/JNK/p53/p21; RPMI8226
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-012-0001-2

This study explored the molecular mechanisms underlying the time-dependent autophagy and apoptosis induced by nutrient depletion in human multiple myeloma cell line RPMI8226 cells.RT-PCR and qRT-PCR were used to evaluate the transcriptional levels of Deptor,JNK1,JNK2,JNK3,Raf-1,p53,p21 and NFκB1 at 0,6,12,18,24 and 48 h after nutrient depletion in RPMI8226 cells.We found that transcriptional levels of Deptor were increased time-dependently at 0,6,12 and 18 h,and then decreased.Its alternation was consistent with autophagy.Transcriptional levels of Raf-1,JNK1,JNK2,p53 and p21 were increased time-dependently at 0,6,12,18,24 and 48 h accompanying with the increase of apoptosis.Transcriptional levels of NFκB1 at 6,12,18,24 and 48 h were decreased as com-pared with 0 h.It was suggested that all the studied signaling molecules were involved in cellular re-sponse to nutrient depletion in RPMI8226 cells.Deptor contributed to autophagy in this process.Raf-1/JNK /p53/p21 pathway may be involved in apoptosis,and NFκB1 may play a possible role in in-hibiting apoptosis.It remained to be studied whether Deptor was involved in both autophagy and apoptosis.