Single-cell DNA methylation and 3D genome architecture in the human brain

• Published in: Science (American Association for the Advancement of Science) Vol. 382; no. 6667; p. eadf5357
• Main Authors: Tian, Wei, Zhou, Jingtian, Bartlett, Anna, Zeng, Qiurui, Liu, Hanqing, Castanon, Rosa G., Kenworthy, Mia, Altshul, Jordan, Valadon, Cynthia, Aldridge, Andrew, Nery, Joseph R., Chen, Huaming, Xu, Jiaying, Johnson, Nicholas D., Lucero, Jacinta, Osteen, Julia K., Emerson, Nora, Rink, Jon, Lee, Jasper, Li, Yang E., Siletti, Kimberly, Liem, Michelle, Claffey, Naomi, O’Connor, Carolyn, Yanny, Anna Marie, Nyhus, Julie, Dee, Nick, Casper, Tamara, Shapovalova, Nadiya, Hirschstein, Daniel, Ding, Song-Lin, Hodge, Rebecca, Levi, Boaz P., Keene, C. Dirk, Linnarsson, Sten, Lein, Ed, Ren, Bing, Behrens, M. Margarita, Ecker, Joseph R.
• Format: Journal Article
• Language: English
• Published: United States The American Association for the Advancement of Science 13.10.2023
• Subjects: Adult; Atlases as Topic; Basal ganglia; Brain; Brain - cytology; Brain - metabolism; Brain architecture; Chromatin; Chromatin - metabolism; Conformation; Deoxyribonucleic acid; DNA; DNA Methylation; Epigenesis, Genetic; Epigenetics; Ganglia; Gene expression; Gene regulation; Genome, Human; Genomes; Humans; Imaging, Three-Dimensional; Male; Neurons; Regulatory mechanisms (biology); Single-Cell Analysis

Delineating the gene-regulatory programs underlying complex cell types is fundamental for understanding brain function in health and disease. Here, we comprehensively examined human brain cell epigenomes by probing DNA methylation and chromatin conformation at single-cell resolution in 517 thousand...