Comparative study of mature and zymogen mite cysteine protease stability and pH unfolding - Chevigné, Andy; Dumez, Marie-Eve; Dumoulin, Mireille; Matagne, André; Jacquet, Alain; Galleni, Moreno (Biochimica et biophysica acta, 2010)
Keywords: Allergen; Animals; Antigens, Dermatophagoides - chemistry; Arthropod Proteins; Biochemistry, biophysics & molecular biology; Biochimie, biophysique & biologie moléculaire; Cysteine Endopeptidases; Cysteine protease; Cysteine Proteases - chemistry; Der p 1; Enzyme Precursors - chemistry; Enzyme Stability; Fluorescence quenching; Hydrogen-Ion Concentration; Life sciences; Mechanism of activation; pH-induced unfolding; Propeptide; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Pyroglyphidae - enzymology; Sciences du vivant; Stability; Thermal denaturation; unfolding; Zymogen; C34A; DTT; Der p 1; ANS; PDC; proDer p 1; AMC; Boc; prop; Mechanism of activation; WT; Fluorescence quenching; PBS; pH-induced unfolding; BMGY; Stability; E-64; Propeptide; Thermal denaturation; EDTA; p; Zymogen; SDS-PAGE; Cysteine protease; Allergen