Quantitative analysis of parasite DNA in the blood of immunized and naïve mice after infection with Neospora caninum

• Published in: Parasitology Vol. 135; no. 2; pp. 175 - 182
• Main Authors: PINITKIATISAKUL, S., MATTSSON, J. G., LUNDÉN, A.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.02.2008
• Subjects: Animals; Antibodies, Protozoan - analysis; Antibodies, Protozoan - metabolism; Antigens, Protozoan - immunology; Antigens, Protozoan - metabolism; Antigens, Surface - immunology; Biological and medical sciences; Body Weight; brain; Brain - parasitology; Coccidiosis - immunology; Coccidiosis - parasitology; disease severity; DNA; DNA, Protozoan - analysis; Female; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; immune-stimulating complexes; immunity; immunization; Invertebrates; iscom; Mice; Mice, Inbred BALB C; NcSRS2; Neospora - immunology; Neospora caninum; neosporosis; parasitaemia; parasite load; parasitemia; polymerase chain reaction; Polymerase Chain Reaction - methods; protective effect; Protozoan Proteins - immunology; Protozoan Vaccines - immunology; quantitative analysis; real-time PCR; real-time polymerase chain reaction; recombinant antigens; recombinant vaccines; Specific Pathogen-Free Organisms; surface antigens; Time Factors; vaccine; Vaccines, Synthetic - immunology; vaccine; iscom; parasitaemia; real-time PCR; Neospora caninum; NcSRS2; Protozoa; Apicomplexa; Rodentia; Parasite; Vaccine; Blood; Infection; Polymerase chain reaction; Vertebrata; Mammalia; Mouse; DNA; Quantitative analysis

Real-time PCR was used to study the duration and level of parasitaemia in mice immunized with immune-stimulating complexes (iscoms) containing recombinant NcSRS2, one of the immunodominant surface antigens of Neospora caninum. After challenge infection, blood was collected daily for 9 days. During t...