Base editing for reprogramming cyanobacterium Synechococcus elongatus
Cyanobacteria can directly convert carbon dioxide (CO2) at the atmospheric level to biofuels, value-added chemicals and food products, making them ideal candidates to alleviate global climate change. Despite decades-long pioneering successes, the development of genome-editing tools, especially the C...
Saved in:
| Published in | Metabolic engineering Vol. 75; pp. 91 - 99 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Belgium
Elsevier Inc
01.01.2023
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Cyanobacteria can directly convert carbon dioxide (CO2) at the atmospheric level to biofuels, value-added chemicals and food products, making them ideal candidates to alleviate global climate change. Despite decades-long pioneering successes, the development of genome-editing tools, especially the CRISPR-Cas-based approaches, seems to lag behind other microbial chassis, slowing down the innovations of cyanobacteria. Here, we adapted and tailored base editing for cyanobacteria based on the CRISPR-Cas system and deamination. We achieved precise and efficient genome editing at a single-nucleotide resolution and demonstrated multiplex base editing in the model cyanobacterium Synechococcus elongatus. By using the base-editing tool, we successfully manipulated the glycogen metabolic pathway via the introduction of premature STOP codons in the relevant genes, building engineered strains with elevated potentials to produce chemicals and food from CO2. We present here the first report of base editing in the phylum of cyanobacteria, and a paradigm for applying CRISPR-Cas systems in bacteria. We believe that our work will accelerate the metabolic engineering and synthetic biology of cyanobacteria and drive more innovations to alleviate global climate change. |
|---|---|
| AbstractList | Cyanobacteria can directly convert carbon dioxide (CO2) at the atmospheric level to biofuels, value-added chemicals and food products, making them ideal candidates to alleviate global climate change. Despite decades-long pioneering successes, the development of genome-editing tools, especially the CRISPR-Cas-based approaches, seems to lag behind other microbial chassis, slowing down the innovations of cyanobacteria. Here, we adapted and tailored base editing for cyanobacteria based on the CRISPR-Cas system and deamination. We achieved precise and efficient genome editing at a single-nucleotide resolution and demonstrated multiplex base editing in the model cyanobacterium Synechococcus elongatus. By using the base-editing tool, we successfully manipulated the glycogen metabolic pathway via the introduction of premature STOP codons in the relevant genes, building engineered strains with elevated potentials to produce chemicals and food from CO2. We present here the first report of base editing in the phylum of cyanobacteria, and a paradigm for applying CRISPR-Cas systems in bacteria. We believe that our work will accelerate the metabolic engineering and synthetic biology of cyanobacteria and drive more innovations to alleviate global climate change. Cyanobacteria can directly convert carbon dioxide (CO ) at the atmospheric level to biofuels, value-added chemicals and food products, making them ideal candidates to alleviate global climate change. Despite decades-long pioneering successes, the development of genome-editing tools, especially the CRISPR-Cas-based approaches, seems to lag behind other microbial chassis, slowing down the innovations of cyanobacteria. Here, we adapted and tailored base editing for cyanobacteria based on the CRISPR-Cas system and deamination. We achieved precise and efficient genome editing at a single-nucleotide resolution and demonstrated multiplex base editing in the model cyanobacterium Synechococcus elongatus. By using the base-editing tool, we successfully manipulated the glycogen metabolic pathway via the introduction of premature STOP codons in the relevant genes, building engineered strains with elevated potentials to produce chemicals and food from CO . We present here the first report of base editing in the phylum of cyanobacteria, and a paradigm for applying CRISPR-Cas systems in bacteria. We believe that our work will accelerate the metabolic engineering and synthetic biology of cyanobacteria and drive more innovations to alleviate global climate change. |
| Author | Wang, Shu-Guang Xia, Peng-Fei Wang, Shu-Yan Li, Xin |
| Author_xml | – sequence: 1 givenname: Shu-Yan surname: Wang fullname: Wang, Shu-Yan organization: School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, China – sequence: 2 givenname: Xin surname: Li fullname: Li, Xin organization: School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, China – sequence: 3 givenname: Shu-Guang surname: Wang fullname: Wang, Shu-Guang organization: School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, China – sequence: 4 givenname: Peng-Fei orcidid: 0000-0001-5881-9834 surname: Xia fullname: Xia, Peng-Fei email: pfxia@sdu.edu.cn organization: School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, China |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/36403709$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9kE1PwzAMhiM0xD7gFyCh_oEWu03a5cABpvEhTeIAnKM0dUantZmSFmn_no7BjpxsWe9jW8-UjVrXEmPXCAkC5rebZN-U1CYppGmCmACIMzZBkHlc4JyPTn2Rj9k0hA0AopB4wcZZziErQE7Y8kEHiqiqu7pdR9b5yNPOu7XXTXOYmL1uXalNR77um-ht35L5dMYZ04eItq5d664Pl-zc6m2gq986Yx-Py_fFc7x6fXpZ3K9iw4F3MaaZKaHgWSokWE2msphXvETJLUnOhSXKq0KDnEuRVgZTEEaUYEWaDSxmM5Yd9xrvQvBk1c7XjfZ7haAOUtRG_UhRBykKUQ1SBurmSO36sqHqxPxZGAJ3xwANv3_V5FUwNbVm0OLJdKpy9b8HvgFyf3Ze |
| CitedBy_id | crossref_primary_10_1021_acssynbio_3c00259 crossref_primary_10_1186_s13068_023_02443_8 crossref_primary_10_1093_plphys_kiae261 crossref_primary_10_1016_j_tibtech_2023_11_010 crossref_primary_10_1021_acssuschemeng_3c05735 crossref_primary_10_3390_microorganisms11020455 crossref_primary_10_1051_bioconf_20236101009 |
| Cites_doi | 10.1038/s41467-020-15491-7 10.1021/acssynbio.2c00375 10.1093/nar/gkq603 10.1073/pnas.1913493116 10.1016/j.ymben.2016.09.006 10.1002/bit.26949 10.1021/acssynbio.0c00226 10.1038/s41587-021-01195-w 10.1021/acssynbio.8b00437 10.1101/gr.107524.110 10.1186/s12934-016-0514-7 10.1111/tpj.12543 10.1002/biot.201800496 10.1038/s43016-021-00340-7 10.1038/s43016-021-00225-9 10.1007/BF00454913 10.1016/j.bbagen.2007.01.006 10.1126/science.aaf8729 10.1016/j.ymben.2021.09.010 10.1016/j.cell.2014.05.010 10.1038/s41467-021-26393-7 10.1007/s11120-014-9987-6 10.1093/bioinformatics/btp324 10.1016/j.tibtech.2021.01.003 10.1128/AEM.65.2.523-528.1999 10.1186/s12934-016-0595-3 10.1016/j.copbio.2021.07.014 10.1021/acssynbio.5b00264 10.1073/pnas.1802940115 10.3389/fmicb.2019.00551 10.1073/pnas.2013338118 10.1016/j.tibtech.2018.05.011 10.1016/j.biotechadv.2022.107946 10.1126/science.aav9973 10.1038/sj.emboj.7600606 10.1016/j.tibtech.2020.09.004 10.1038/s41467-022-30780-z 10.1016/j.ymben.2018.03.014 10.1128/AEM.02023-17 10.1016/j.cell.2020.03.023 10.1016/0076-6879(87)53055-5 10.1038/s41586-020-1978-5 10.1093/bioinformatics/btp352 10.1038/s41564-017-0102-6 10.1038/nature17946 10.1038/s41467-020-15384-9 10.1099/mic.0.000377 10.1038/nature24644 10.1039/C9EE01214A 10.1016/j.biotechadv.2019.04.005 10.1016/j.tibtech.2015.03.009 10.3389/fbioe.2022.897204 10.1007/978-1-59745-257-1_11 10.1038/s41598-017-17035-4 10.1073/pnas.1800828115 10.1038/s41576-018-0059-1 |
| ContentType | Journal Article |
| Copyright | 2022 International Metabolic Engineering Society Copyright © 2022 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved. |
| Copyright_xml | – notice: 2022 International Metabolic Engineering Society – notice: Copyright © 2022 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved. |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION |
| DOI | 10.1016/j.ymben.2022.11.005 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef |
| DatabaseTitleList | MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Engineering Biology |
| EISSN | 1096-7184 |
| EndPage | 99 |
| ExternalDocumentID | 10_1016_j_ymben_2022_11_005 36403709 S1096717622001483 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- --K --M .~1 0R~ 123 1B1 1RT 1~. 1~5 29M 4.4 457 4G. 53G 5VS 7-5 71M 8P~ AAAJQ AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARKO AAXUO ABFNM ABGSF ABJNI ABMAC ABNUV ABUDA ABXDB ABYKQ ACDAQ ACGFS ACRLP ADBBV ADEWK ADEZE ADFGL ADMUD ADUVX AEBSH AEHWI AEKER AENEX AFKWA AFTJW AFXIZ AGEKW AGHFR AGRDE AGUBO AGYEJ AHPOS AIEXJ AIKHN AITUG AJBFU AJOXV AKURH ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC CAG CJTIS COF CS3 DM4 DOVZS DU5 EBS EFBJH EFLBG EJD ENUVR EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-Q GBLVA HVGLF HZ~ IHE J1W KOM LG5 LUGTX M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SDF SDG SDP SES SEW SPC SPCBC SSG SSI SSU SSZ T5K UHS XPP ZMT ZU3 ~G- AAHBH AAXKI AFJKZ AKRWK CGR CUY CVF ECM EIF NPM AAYXX CITATION |
| ID | FETCH-LOGICAL-c404t-123cb07432590faecdf16d4b194fe9445fee6d7a098952dc1205c5b0f52312313 |
| IEDL.DBID | .~1 |
| ISSN | 1096-7176 |
| IngestDate | Thu Sep 26 16:20:01 EDT 2024 Sat Sep 28 08:17:37 EDT 2024 Fri Feb 23 02:39:29 EST 2024 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | CRISPR Synechococcus elongatus Base editing Multiplex Cyanobacteria |
| Language | English |
| License | Copyright © 2022 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c404t-123cb07432590faecdf16d4b194fe9445fee6d7a098952dc1205c5b0f52312313 |
| ORCID | 0000-0001-5881-9834 |
| OpenAccessLink | https://doi.org/10.1016/j.ymben.2022.11.005 |
| PMID | 36403709 |
| PageCount | 9 |
| ParticipantIDs | crossref_primary_10_1016_j_ymben_2022_11_005 pubmed_primary_36403709 elsevier_sciencedirect_doi_10_1016_j_ymben_2022_11_005 |
| PublicationCentury | 2000 |
| PublicationDate | January 2023 2023-01-00 |
| PublicationDateYYYYMMDD | 2023-01-01 |
| PublicationDate_xml | – month: 01 year: 2023 text: January 2023 |
| PublicationDecade | 2020 |
| PublicationPlace | Belgium |
| PublicationPlace_xml | – name: Belgium |
| PublicationTitle | Metabolic engineering |
| PublicationTitleAlternate | Metab Eng |
| PublicationYear | 2023 |
| Publisher | Elsevier Inc |
| Publisher_xml | – name: Elsevier Inc |
| References | Li, Handsaker, Wysoker, Fennell, Ruan, Homer, Marth, Abecasis, Durbin (bib26) 2009; 25 Taton, Erikson, Yang, Rubin, Rifkin, Golden, Golden (bib45) 2020; 11 Ernst, Kirschenlohr, Diez, Böger (bib15) 1984; 140 Wichmann, Lauersen, Biondi, Christensen, Guerra, Hellgardt, Kuhner, Kuronen, Lindberg, Rosch, Yunus, Jones, Lindblad, Kruse (bib52) 2021; 39 Crippa, Solazzo, Guizzardi, Monforti-Ferrario, Tubiello, Leip (bib12) 2021; 2 Li, Durbin (bib25) 2009; 25 Liu, Xie, Roussou, Lindblad (bib32) 2022; 73 Ivleva, Bramlett, Lindahl, Golden (bib21) 2005; 24 Qiao, Duan, Zhang, Hagemann, Luo, Lu (bib39) 2018; 84 Yao, Cengic, Anfelt, Hudson (bib55) 2016; 5 Suzuki, Umeda, Nihei, Moriya, Ohkawa, Fujiwara, Tsuzuki, Nakamura (bib44) 2007; 1770 Welkie, Rubin, Chang, Diamond, Rifkin, LiWang, Golden (bib50) 2018; 115 Li, Shen, Huang, Sung, Wu, Hu (bib27) 2016; 38 Rodrigues, Karimi, Impens, Van Lerberge, Coussens, Aesaert, Rombaut, Holtappels, Ibrahim, Van Montagu, Wagemans, Jacobs, De Coninck, Pauwels (bib41) 2021; 118 Yu, Wang, Xia (bib57) 2022; 10 Muscat, de Olde, Ripoll-Bosch, Van Zanten, Metze, Termeer, van Ittersum, de Boer (bib36) 2021; 2 Zuo, Sun, Wei, Yuan, Ying, Sun, Yuan, Steinmetz, Li, Yang (bib58) 2019; 364 Shabestary, Hernandez, Miao, Ljungqvist, Hallman, Sporre, Branco Dos Santos, Hudson (bib43) 2021; 68 Cao, Li, Xia, Wei, Guo, Li, Wang (bib5) 2017; 7 McKenna, Hanna, Banks, Sivachenko, Cibulskis, Kernytsky, Garimella, Altshuler, Gabriel, Daly, DePristo (bib35) 2010; 20 Doudna (bib14) 2020; 578 Rees, Liu (bib40) 2018; 19 Xia, Casini, Schulz, Klask, Angenent, Molitor (bib53) 2020; 9 Xia, Ling, Foo, Chang (bib54) 2019; 14 Li, Seys, Minton, Yang, Jiang, Jiang, Yang (bib28) 2019; 116 Clerico, Ditty, Golden (bib10) 2007; 362 Cengic, Canadas, Minton, Hudson (bib7) 2022; 11 Volke, Martino, Kozaeva, Smania, Nikel (bib47) 2022; 13 Chen, Taton, Go, London, Pieper, Golden, Golden (bib8) 2016; 162 Nishida, Arazoe, Yachie, Banno, Kakimoto, Tabata, Mochizuki, Miyabe, Araki, Hara, Shimatani, Kondo (bib37) 2016; 353 Cohen, McKnight, Golden (bib11) 2018; 115 Luan, Zhang, Wang, Lu (bib33) 2019; 37 Tong, Whitford, Robertsen, Blin, Jorgensen, Klitgaard, Gren, Jiang, Weber, Lee (bib46) 2019; 116 Ahn, Lee, Choi, Bang, Lee (bib1) 2022 Yao, Shabestary, Bjork, Asplund-Samuelsson, Joensson, Jahn, Hudson (bib56) 2020; 11 Mapstone, Leite, Purton, Crawford, Dartnell (bib34) 2022; 59 Kruyer, Realff, Sun, Genzale, Peralta-Yahya (bib24) 2021; 12 Sendersky, Kozer, Levi, Garini, Shav-Tal, Schwarz (bib42) 2014; 79 Behler, Vijay, Hess, Akhtar (bib4) 2018; 36 Deng, Coleman (bib13) 1999; 65 Kiefer, Merkel, Lilge, Henkel, Hausmann (bib22) 2021; 39 Godar, Kamoku, Nielsen, Wang (bib17) 2021; 24 Wendt, Ungerer, Cobb, Zhao, Pakrasi (bib51) 2016; 15 Carroll, Case, Zhang, Atsumi (bib6) 2018; 50 Gaudelli, Komor, Rees, Packer, Badran, Bryson, Liu (bib16) 2017; 551 Chi, Zhang, Sun, Duan, Qiao, Luan, Lu (bib9) 2019; 10 Li, Shen, Liao (bib29) 2014; 120 Niu, Lin, Xie, Wang, Xing, Zhang, Zhang (bib38) 2019; 8 Wang, Zhang, Gao (bib48) 2020; 181 Angermayr, Gorchs Rovira, Hellingwerf (bib2) 2015; 33 Banno, Nishida, Arazoe, Mitsunobu, Kondo (bib3) 2018; 3 Liew, Nogle, Abdalla, Rasor, Canter, Jensen, Wang, Strutz, Chirania, De Tissera, Mueller, Ruan, Gao, Tran, Engle, Bromley, Daniell, Conrado, Tschaplinski, Giannone, Hettich, Karim, Simpson, Brown, Leang, Jewett, Kopke (bib30) 2022; 40 Liu, Miao, Lindberg, Lindblad (bib31) 2019; 12 Wang, Li, Hakonarson (bib49) 2010; 38 Golden, Brusslan, Haselkorn (bib18) 1987; 153 Huang, Shen, Li, Sung, Wu, Hu (bib20) 2016; 15 Hsu, Lander, Zhang (bib19) 2014; 157 Komor, Kim, Packer, Zuris, Liu (bib23) 2016; 533 Volke (10.1016/j.ymben.2022.11.005_bib47) 2022; 13 Li (10.1016/j.ymben.2022.11.005_bib26) 2009; 25 Shabestary (10.1016/j.ymben.2022.11.005_bib43) 2021; 68 Luan (10.1016/j.ymben.2022.11.005_bib33) 2019; 37 Komor (10.1016/j.ymben.2022.11.005_bib23) 2016; 533 Yao (10.1016/j.ymben.2022.11.005_bib56) 2020; 11 Liu (10.1016/j.ymben.2022.11.005_bib31) 2019; 12 Ahn (10.1016/j.ymben.2022.11.005_bib1) 2022 Cao (10.1016/j.ymben.2022.11.005_bib5) 2017; 7 Godar (10.1016/j.ymben.2022.11.005_bib17) 2021; 24 Li (10.1016/j.ymben.2022.11.005_bib25) 2009; 25 Li (10.1016/j.ymben.2022.11.005_bib28) 2019; 116 Doudna (10.1016/j.ymben.2022.11.005_bib14) 2020; 578 Yu (10.1016/j.ymben.2022.11.005_bib57) 2022; 10 Muscat (10.1016/j.ymben.2022.11.005_bib36) 2021; 2 Chen (10.1016/j.ymben.2022.11.005_bib8) 2016; 162 Li (10.1016/j.ymben.2022.11.005_bib29) 2014; 120 Carroll (10.1016/j.ymben.2022.11.005_bib6) 2018; 50 Xia (10.1016/j.ymben.2022.11.005_bib54) 2019; 14 Tong (10.1016/j.ymben.2022.11.005_bib46) 2019; 116 Welkie (10.1016/j.ymben.2022.11.005_bib50) 2018; 115 Behler (10.1016/j.ymben.2022.11.005_bib4) 2018; 36 Li (10.1016/j.ymben.2022.11.005_bib27) 2016; 38 Liu (10.1016/j.ymben.2022.11.005_bib32) 2022; 73 Kruyer (10.1016/j.ymben.2022.11.005_bib24) 2021; 12 Sendersky (10.1016/j.ymben.2022.11.005_bib42) 2014; 79 Zuo (10.1016/j.ymben.2022.11.005_bib58) 2019; 364 Deng (10.1016/j.ymben.2022.11.005_bib13) 1999; 65 Chi (10.1016/j.ymben.2022.11.005_bib9) 2019; 10 Wendt (10.1016/j.ymben.2022.11.005_bib51) 2016; 15 Cengic (10.1016/j.ymben.2022.11.005_bib7) 2022; 11 Ernst (10.1016/j.ymben.2022.11.005_bib15) 1984; 140 Rees (10.1016/j.ymben.2022.11.005_bib40) 2018; 19 Wichmann (10.1016/j.ymben.2022.11.005_bib52) 2021; 39 Banno (10.1016/j.ymben.2022.11.005_bib3) 2018; 3 Gaudelli (10.1016/j.ymben.2022.11.005_bib16) 2017; 551 Hsu (10.1016/j.ymben.2022.11.005_bib19) 2014; 157 Xia (10.1016/j.ymben.2022.11.005_bib53) 2020; 9 Qiao (10.1016/j.ymben.2022.11.005_bib39) 2018; 84 Wang (10.1016/j.ymben.2022.11.005_bib49) 2010; 38 Huang (10.1016/j.ymben.2022.11.005_bib20) 2016; 15 Liew (10.1016/j.ymben.2022.11.005_bib30) 2022; 40 Nishida (10.1016/j.ymben.2022.11.005_bib37) 2016; 353 Wang (10.1016/j.ymben.2022.11.005_bib48) 2020; 181 McKenna (10.1016/j.ymben.2022.11.005_bib35) 2010; 20 Rodrigues (10.1016/j.ymben.2022.11.005_bib41) 2021; 118 Ivleva (10.1016/j.ymben.2022.11.005_bib21) 2005; 24 Niu (10.1016/j.ymben.2022.11.005_bib38) 2019; 8 Golden (10.1016/j.ymben.2022.11.005_bib18) 1987; 153 Suzuki (10.1016/j.ymben.2022.11.005_bib44) 2007; 1770 Cohen (10.1016/j.ymben.2022.11.005_bib11) 2018; 115 Kiefer (10.1016/j.ymben.2022.11.005_bib22) 2021; 39 Taton (10.1016/j.ymben.2022.11.005_bib45) 2020; 11 Angermayr (10.1016/j.ymben.2022.11.005_bib2) 2015; 33 Yao (10.1016/j.ymben.2022.11.005_bib55) 2016; 5 Mapstone (10.1016/j.ymben.2022.11.005_bib34) 2022; 59 Clerico (10.1016/j.ymben.2022.11.005_bib10) 2007; 362 Crippa (10.1016/j.ymben.2022.11.005_bib12) 2021; 2 |
| References_xml | – volume: 38 start-page: 293 year: 2016 end-page: 302 ident: bib27 article-title: CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production publication-title: Metab. Eng. contributor: fullname: Hu – volume: 8 start-page: 170 year: 2019 end-page: 180 ident: bib38 article-title: Expanding the potential of CRISPR-Cpf1-based genome editing technology in the cyanobacterium publication-title: ACS Synth. Biol. contributor: fullname: Zhang – volume: 115 start-page: E7805 year: 2018 end-page: E7813 ident: bib11 article-title: Roles for ClpXP in regulating the circadian clock in publication-title: Proc. Natl. Acad. Sci. U. S. A. contributor: fullname: Golden – volume: 25 start-page: 1754 year: 2009 end-page: 1760 ident: bib25 article-title: Fast and accurate short read alignment with Burrows-Wheeler transform publication-title: Bioinformatics contributor: fullname: Durbin – volume: 118 year: 2021 ident: bib41 article-title: Efficient CRISPR-mediated base editing in publication-title: Proc. Natl. Acad. Sci. U. S. A. contributor: fullname: Pauwels – volume: 362 start-page: 155 year: 2007 end-page: 171 ident: bib10 article-title: Specialized techniques for site-directed mutagenesis in cyanobacteria publication-title: Methods Mol. Biol. contributor: fullname: Golden – volume: 50 start-page: 47 year: 2018 end-page: 56 ident: bib6 article-title: Metabolic engineering tools in model cyanobacteria publication-title: Metab. Eng. contributor: fullname: Atsumi – volume: 40 start-page: 335 year: 2022 end-page: 344 ident: bib30 article-title: Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale publication-title: Nat. Biotechnol. contributor: fullname: Kopke – volume: 353 year: 2016 ident: bib37 article-title: Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems publication-title: Science contributor: fullname: Kondo – volume: 364 start-page: 289 year: 2019 end-page: 292 ident: bib58 article-title: Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos publication-title: Science contributor: fullname: Yang – volume: 153 start-page: 215 year: 1987 end-page: 231 ident: bib18 article-title: Genetic engineering of the cyanobacterial chromosome publication-title: Methods Enzymol. contributor: fullname: Haselkorn – volume: 10 start-page: 551 year: 2019 ident: bib9 article-title: Adopting a theophylline-responsive riboswitch for flexible regulation and understanding of glycogen metabolism in publication-title: Front. Microbiol. contributor: fullname: Lu – volume: 38 start-page: e164 year: 2010 ident: bib49 article-title: ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data publication-title: Nucleic Acids Res. contributor: fullname: Hakonarson – volume: 39 start-page: 397 year: 2021 end-page: 411 ident: bib22 article-title: From acetate to bio-based products: underexploited potential for industrial biotechnology publication-title: Trends Biotechnol. contributor: fullname: Hausmann – volume: 20 start-page: 1297 year: 2010 end-page: 1303 ident: bib35 article-title: The genome analysis toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data publication-title: Genome Res. contributor: fullname: DePristo – volume: 11 start-page: 1666 year: 2020 ident: bib56 article-title: Pooled CRISPRi screening of the cyanobacterium publication-title: Nat. Commun. contributor: fullname: Hudson – volume: 578 start-page: 229 year: 2020 end-page: 236 ident: bib14 article-title: The promise and challenge of therapeutic genome editing publication-title: Nature contributor: fullname: Doudna – volume: 3 start-page: 423 year: 2018 end-page: 429 ident: bib3 article-title: Deaminase-mediated multiplex genome editing in publication-title: Nat. Microbiol. contributor: fullname: Kondo – volume: 12 start-page: 2765 year: 2019 end-page: 2777 ident: bib31 article-title: Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO publication-title: Energy Environ. Sci. contributor: fullname: Lindblad – volume: 533 start-page: 420 year: 2016 end-page: 424 ident: bib23 article-title: Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage publication-title: Nature contributor: fullname: Liu – volume: 25 start-page: 2078 year: 2009 end-page: 2079 ident: bib26 article-title: The sequence alignment/map format and SAMtools publication-title: Bioinformatics contributor: fullname: Durbin – year: 2022 ident: bib1 article-title: Can microbes be harnessed to reduce atmospheric loads of greenhouse gases? publication-title: Environ. Microbiol. contributor: fullname: Lee – volume: 84 year: 2018 ident: bib39 article-title: Effects of reduced and enhanced glycogen pools on salt-induced sucrose production in a sucrose-secreting strain of publication-title: Appl. Environ. Microbiol. contributor: fullname: Lu – volume: 79 start-page: 118 year: 2014 end-page: 126 ident: bib42 article-title: The proteolysis adaptor, NblA, initiates protein pigment degradation by interacting with the cyanobacterial light-harvesting complexes publication-title: Plant J. contributor: fullname: Schwarz – volume: 2 start-page: 561 year: 2021 end-page: 566 ident: bib36 article-title: Principles, drivers and opportunities of a circular bioeconomy publication-title: Nature Food contributor: fullname: de Boer – volume: 10 year: 2022 ident: bib57 article-title: Reprogramming microbial CO publication-title: Front. Bioeng. Biotechnol. contributor: fullname: Xia – volume: 116 start-page: 1475 year: 2019 end-page: 1483 ident: bib28 article-title: CRISPR-Cas9(D10A) nickase-assisted base editing in the solvent producer publication-title: Biotechnol. Bioeng. contributor: fullname: Yang – volume: 12 start-page: 6166 year: 2021 ident: bib24 article-title: Designing the bioproduction of Martian rocket propellant via a biotechnology-enabled in situ resource utilization strategy publication-title: Nat. Commun. contributor: fullname: Peralta-Yahya – volume: 13 start-page: 3026 year: 2022 ident: bib47 article-title: Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing publication-title: Nat. Commun. contributor: fullname: Nikel – volume: 115 start-page: E7174 year: 2018 end-page: E7183 ident: bib50 article-title: Genome-wide fitness assessment during diurnal growth reveals an expanded role of the cyanobacterial circadian clock protein KaiA publication-title: Proc. Natl. Acad. Sci. U. S. A. contributor: fullname: Golden – volume: 65 start-page: 523 year: 1999 end-page: 528 ident: bib13 article-title: Ethanol synthesis by genetic engineering in cyanobacteria publication-title: Appl. Environ. Microbiol. contributor: fullname: Coleman – volume: 11 start-page: 1688 year: 2020 ident: bib45 article-title: The circadian clock and darkness control natural competence in cyanobacteria publication-title: Nat. Commun. contributor: fullname: Golden – volume: 33 start-page: 352 year: 2015 end-page: 361 ident: bib2 article-title: Metabolic engineering of cyanobacteria for the synthesis of commodity products publication-title: Trends Biotechnol. contributor: fullname: Hellingwerf – volume: 162 start-page: 2029 year: 2016 end-page: 2041 ident: bib8 article-title: Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium publication-title: Microbiology contributor: fullname: Golden – volume: 37 start-page: 771 year: 2019 end-page: 786 ident: bib33 article-title: Progress and perspective on cyanobacterial glycogen metabolism engineering publication-title: Biotechnol. Adv. contributor: fullname: Lu – volume: 1770 start-page: 763 year: 2007 end-page: 773 ident: bib44 article-title: Role of the GlgX protein in glycogen metabolism of the cyanobacterium, publication-title: Biochim. Biophys. Acta contributor: fullname: Nakamura – volume: 14 year: 2019 ident: bib54 article-title: Synthetic biology toolkits for metabolic engineering of cyanobacteria publication-title: Biotechnol. J. contributor: fullname: Chang – volume: 73 start-page: 143 year: 2022 end-page: 150 ident: bib32 article-title: Current advances in engineering cyanobacteria and their applications for photosynthetic butanol production publication-title: Curr. Opin. Biotechnol. contributor: fullname: Lindblad – volume: 19 start-page: 770 year: 2018 end-page: 788 ident: bib40 article-title: Base editing: precision chemistry on the genome and transcriptome of living cells publication-title: Nat. Rev. Genet. contributor: fullname: Liu – volume: 39 start-page: 323 year: 2021 end-page: 327 ident: bib52 article-title: Engineering biocatalytic solar fuel production: the PHOTOFUEL consortium publication-title: Trends Biotechnol. contributor: fullname: Kruse – volume: 140 start-page: 120 year: 1984 end-page: 125 ident: bib15 article-title: Glycogen content and nitrogenase activity in publication-title: Arch. Microbiol. contributor: fullname: Böger – volume: 24 start-page: 1202 year: 2005 end-page: 1210 ident: bib21 article-title: LdpA: a component of the circadian clock senses redox state of the cell publication-title: EMBO J. contributor: fullname: Golden – volume: 9 start-page: 2162 year: 2020 end-page: 2171 ident: bib53 article-title: Reprogramming acetogenic bacteria with CRISPR-targeted base editing via deamination publication-title: ACS Synth. Biol. contributor: fullname: Molitor – volume: 120 start-page: 301 year: 2014 end-page: 310 ident: bib29 article-title: Isobutanol production as an alternative metabolic sink to rescue the growth deficiency of the glycogen mutant of publication-title: Photosynth. Res. contributor: fullname: Liao – volume: 7 year: 2017 ident: bib5 article-title: AraBAD based toolkit for gene expression and metabolic robustness improvement in publication-title: Sci. Rep. contributor: fullname: Wang – volume: 59 year: 2022 ident: bib34 article-title: Cyanobacteria and microalgae in supporting human habitation on Mars publication-title: Biotechnol. Adv. contributor: fullname: Dartnell – volume: 68 start-page: 131 year: 2021 end-page: 141 ident: bib43 article-title: Cycling between growth and production phases increases cyanobacteria bioproduction of lactate publication-title: Metab. Eng. contributor: fullname: Hudson – volume: 36 start-page: 996 year: 2018 end-page: 1010 ident: bib4 article-title: CRISPR-based technologies for metabolic engineering in cyanobacteria publication-title: Trends Biotechnol. contributor: fullname: Akhtar – volume: 24 year: 2021 ident: bib17 article-title: Synthetic biology strategies to address waste CO publication-title: Curr. Opin. Environ. Sci. Health contributor: fullname: Wang – volume: 116 start-page: 20366 year: 2019 end-page: 20375 ident: bib46 article-title: Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST publication-title: Proc. Natl. Acad. Sci. U. S. A. contributor: fullname: Lee – volume: 15 start-page: 196 year: 2016 ident: bib20 article-title: CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium publication-title: Microb. Cell. Fact. contributor: fullname: Hu – volume: 181 start-page: 136 year: 2020 end-page: 150 ident: bib48 article-title: CRISPR-based therapeutic genome editing: strategies and in vivo delivery by AAV vectors publication-title: Cell contributor: fullname: Gao – volume: 157 start-page: 1262 year: 2014 end-page: 1278 ident: bib19 article-title: Development and applications of CRISPR-Cas9 for genome engineering publication-title: Cell contributor: fullname: Zhang – volume: 5 start-page: 207 year: 2016 end-page: 212 ident: bib55 article-title: Multiple gene repression in cyanobacteria using CRISPRi publication-title: ACS Synth. Biol. contributor: fullname: Hudson – volume: 2 start-page: 198 year: 2021 end-page: 209 ident: bib12 article-title: Food systems are responsible for a third of global anthropogenic GHG emissions publication-title: Nature Food contributor: fullname: Leip – volume: 551 start-page: 464 year: 2017 end-page: 471 ident: bib16 article-title: Programmable base editing of A⋅T to G⋅C in genomic DNA without DNA cleavage publication-title: Nature contributor: fullname: Liu – volume: 11 start-page: 3100 year: 2022 end-page: 3113 ident: bib7 article-title: Inducible CRISPR/Cas9 allows for multiplexed and rapidly segregated single-target genome editing in publication-title: ACS Synth. Biol. contributor: fullname: Hudson – volume: 15 start-page: 115 year: 2016 ident: bib51 article-title: CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium publication-title: Microb. Cell. Fact. contributor: fullname: Pakrasi – volume: 11 start-page: 1666 year: 2020 ident: 10.1016/j.ymben.2022.11.005_bib56 article-title: Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes publication-title: Nat. Commun. doi: 10.1038/s41467-020-15491-7 contributor: fullname: Yao – volume: 11 start-page: 3100 year: 2022 ident: 10.1016/j.ymben.2022.11.005_bib7 article-title: Inducible CRISPR/Cas9 allows for multiplexed and rapidly segregated single-target genome editing in Synechocystis Sp. PCC 6803 publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.2c00375 contributor: fullname: Cengic – volume: 38 start-page: e164 year: 2010 ident: 10.1016/j.ymben.2022.11.005_bib49 article-title: ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkq603 contributor: fullname: Wang – volume: 116 start-page: 20366 year: 2019 ident: 10.1016/j.ymben.2022.11.005_bib46 article-title: Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.1913493116 contributor: fullname: Tong – volume: 38 start-page: 293 year: 2016 ident: 10.1016/j.ymben.2022.11.005_bib27 article-title: CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production publication-title: Metab. Eng. doi: 10.1016/j.ymben.2016.09.006 contributor: fullname: Li – volume: 116 start-page: 1475 year: 2019 ident: 10.1016/j.ymben.2022.11.005_bib28 article-title: CRISPR-Cas9(D10A) nickase-assisted base editing in the solvent producer Clostridium beijerinckii publication-title: Biotechnol. Bioeng. doi: 10.1002/bit.26949 contributor: fullname: Li – volume: 9 start-page: 2162 year: 2020 ident: 10.1016/j.ymben.2022.11.005_bib53 article-title: Reprogramming acetogenic bacteria with CRISPR-targeted base editing via deamination publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.0c00226 contributor: fullname: Xia – volume: 40 start-page: 335 year: 2022 ident: 10.1016/j.ymben.2022.11.005_bib30 article-title: Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale publication-title: Nat. Biotechnol. doi: 10.1038/s41587-021-01195-w contributor: fullname: Liew – volume: 8 start-page: 170 year: 2019 ident: 10.1016/j.ymben.2022.11.005_bib38 article-title: Expanding the potential of CRISPR-Cpf1-based genome editing technology in the cyanobacterium Anabaena PCC 7120 publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.8b00437 contributor: fullname: Niu – volume: 20 start-page: 1297 year: 2010 ident: 10.1016/j.ymben.2022.11.005_bib35 article-title: The genome analysis toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data publication-title: Genome Res. doi: 10.1101/gr.107524.110 contributor: fullname: McKenna – volume: 15 start-page: 115 year: 2016 ident: 10.1016/j.ymben.2022.11.005_bib51 article-title: CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973 publication-title: Microb. Cell. Fact. doi: 10.1186/s12934-016-0514-7 contributor: fullname: Wendt – volume: 79 start-page: 118 year: 2014 ident: 10.1016/j.ymben.2022.11.005_bib42 article-title: The proteolysis adaptor, NblA, initiates protein pigment degradation by interacting with the cyanobacterial light-harvesting complexes publication-title: Plant J. doi: 10.1111/tpj.12543 contributor: fullname: Sendersky – volume: 14 year: 2019 ident: 10.1016/j.ymben.2022.11.005_bib54 article-title: Synthetic biology toolkits for metabolic engineering of cyanobacteria publication-title: Biotechnol. J. doi: 10.1002/biot.201800496 contributor: fullname: Xia – volume: 2 start-page: 561 year: 2021 ident: 10.1016/j.ymben.2022.11.005_bib36 article-title: Principles, drivers and opportunities of a circular bioeconomy publication-title: Nature Food doi: 10.1038/s43016-021-00340-7 contributor: fullname: Muscat – volume: 2 start-page: 198 year: 2021 ident: 10.1016/j.ymben.2022.11.005_bib12 article-title: Food systems are responsible for a third of global anthropogenic GHG emissions publication-title: Nature Food doi: 10.1038/s43016-021-00225-9 contributor: fullname: Crippa – volume: 140 start-page: 120 year: 1984 ident: 10.1016/j.ymben.2022.11.005_bib15 article-title: Glycogen content and nitrogenase activity in Anabaena variabilis publication-title: Arch. Microbiol. doi: 10.1007/BF00454913 contributor: fullname: Ernst – volume: 1770 start-page: 763 year: 2007 ident: 10.1016/j.ymben.2022.11.005_bib44 article-title: Role of the GlgX protein in glycogen metabolism of the cyanobacterium, Synechococcus elongatus PCC 7942 publication-title: Biochim. Biophys. Acta doi: 10.1016/j.bbagen.2007.01.006 contributor: fullname: Suzuki – volume: 353 year: 2016 ident: 10.1016/j.ymben.2022.11.005_bib37 article-title: Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems publication-title: Science doi: 10.1126/science.aaf8729 contributor: fullname: Nishida – volume: 68 start-page: 131 year: 2021 ident: 10.1016/j.ymben.2022.11.005_bib43 article-title: Cycling between growth and production phases increases cyanobacteria bioproduction of lactate publication-title: Metab. Eng. doi: 10.1016/j.ymben.2021.09.010 contributor: fullname: Shabestary – volume: 157 start-page: 1262 year: 2014 ident: 10.1016/j.ymben.2022.11.005_bib19 article-title: Development and applications of CRISPR-Cas9 for genome engineering publication-title: Cell doi: 10.1016/j.cell.2014.05.010 contributor: fullname: Hsu – volume: 12 start-page: 6166 year: 2021 ident: 10.1016/j.ymben.2022.11.005_bib24 article-title: Designing the bioproduction of Martian rocket propellant via a biotechnology-enabled in situ resource utilization strategy publication-title: Nat. Commun. doi: 10.1038/s41467-021-26393-7 contributor: fullname: Kruyer – volume: 120 start-page: 301 year: 2014 ident: 10.1016/j.ymben.2022.11.005_bib29 article-title: Isobutanol production as an alternative metabolic sink to rescue the growth deficiency of the glycogen mutant of Synechococcus elongatus PCC 7942 publication-title: Photosynth. Res. doi: 10.1007/s11120-014-9987-6 contributor: fullname: Li – volume: 25 start-page: 1754 year: 2009 ident: 10.1016/j.ymben.2022.11.005_bib25 article-title: Fast and accurate short read alignment with Burrows-Wheeler transform publication-title: Bioinformatics doi: 10.1093/bioinformatics/btp324 contributor: fullname: Li – volume: 39 start-page: 323 year: 2021 ident: 10.1016/j.ymben.2022.11.005_bib52 article-title: Engineering biocatalytic solar fuel production: the PHOTOFUEL consortium publication-title: Trends Biotechnol. doi: 10.1016/j.tibtech.2021.01.003 contributor: fullname: Wichmann – volume: 65 start-page: 523 year: 1999 ident: 10.1016/j.ymben.2022.11.005_bib13 article-title: Ethanol synthesis by genetic engineering in cyanobacteria publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.65.2.523-528.1999 contributor: fullname: Deng – volume: 15 start-page: 196 year: 2016 ident: 10.1016/j.ymben.2022.11.005_bib20 article-title: CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942 publication-title: Microb. Cell. Fact. doi: 10.1186/s12934-016-0595-3 contributor: fullname: Huang – volume: 73 start-page: 143 year: 2022 ident: 10.1016/j.ymben.2022.11.005_bib32 article-title: Current advances in engineering cyanobacteria and their applications for photosynthetic butanol production publication-title: Curr. Opin. Biotechnol. doi: 10.1016/j.copbio.2021.07.014 contributor: fullname: Liu – volume: 5 start-page: 207 year: 2016 ident: 10.1016/j.ymben.2022.11.005_bib55 article-title: Multiple gene repression in cyanobacteria using CRISPRi publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.5b00264 contributor: fullname: Yao – volume: 115 start-page: E7174 year: 2018 ident: 10.1016/j.ymben.2022.11.005_bib50 article-title: Genome-wide fitness assessment during diurnal growth reveals an expanded role of the cyanobacterial circadian clock protein KaiA publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.1802940115 contributor: fullname: Welkie – volume: 10 start-page: 551 year: 2019 ident: 10.1016/j.ymben.2022.11.005_bib9 article-title: Adopting a theophylline-responsive riboswitch for flexible regulation and understanding of glycogen metabolism in Synechococcus elongatus PCC7942 publication-title: Front. Microbiol. doi: 10.3389/fmicb.2019.00551 contributor: fullname: Chi – volume: 118 year: 2021 ident: 10.1016/j.ymben.2022.11.005_bib41 article-title: Efficient CRISPR-mediated base editing in Agrobacterium spp publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.2013338118 contributor: fullname: Rodrigues – volume: 36 start-page: 996 year: 2018 ident: 10.1016/j.ymben.2022.11.005_bib4 article-title: CRISPR-based technologies for metabolic engineering in cyanobacteria publication-title: Trends Biotechnol. doi: 10.1016/j.tibtech.2018.05.011 contributor: fullname: Behler – volume: 59 year: 2022 ident: 10.1016/j.ymben.2022.11.005_bib34 article-title: Cyanobacteria and microalgae in supporting human habitation on Mars publication-title: Biotechnol. Adv. doi: 10.1016/j.biotechadv.2022.107946 contributor: fullname: Mapstone – volume: 364 start-page: 289 year: 2019 ident: 10.1016/j.ymben.2022.11.005_bib58 article-title: Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos publication-title: Science doi: 10.1126/science.aav9973 contributor: fullname: Zuo – volume: 24 start-page: 1202 year: 2005 ident: 10.1016/j.ymben.2022.11.005_bib21 article-title: LdpA: a component of the circadian clock senses redox state of the cell publication-title: EMBO J. doi: 10.1038/sj.emboj.7600606 contributor: fullname: Ivleva – volume: 39 start-page: 397 year: 2021 ident: 10.1016/j.ymben.2022.11.005_bib22 article-title: From acetate to bio-based products: underexploited potential for industrial biotechnology publication-title: Trends Biotechnol. doi: 10.1016/j.tibtech.2020.09.004 contributor: fullname: Kiefer – volume: 13 start-page: 3026 year: 2022 ident: 10.1016/j.ymben.2022.11.005_bib47 article-title: Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing publication-title: Nat. Commun. doi: 10.1038/s41467-022-30780-z contributor: fullname: Volke – volume: 50 start-page: 47 year: 2018 ident: 10.1016/j.ymben.2022.11.005_bib6 article-title: Metabolic engineering tools in model cyanobacteria publication-title: Metab. Eng. doi: 10.1016/j.ymben.2018.03.014 contributor: fullname: Carroll – volume: 84 year: 2018 ident: 10.1016/j.ymben.2022.11.005_bib39 article-title: Effects of reduced and enhanced glycogen pools on salt-induced sucrose production in a sucrose-secreting strain of Synechococcus elongatus PCC 7942 publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.02023-17 contributor: fullname: Qiao – volume: 181 start-page: 136 year: 2020 ident: 10.1016/j.ymben.2022.11.005_bib48 article-title: CRISPR-based therapeutic genome editing: strategies and in vivo delivery by AAV vectors publication-title: Cell doi: 10.1016/j.cell.2020.03.023 contributor: fullname: Wang – volume: 153 start-page: 215 year: 1987 ident: 10.1016/j.ymben.2022.11.005_bib18 article-title: Genetic engineering of the cyanobacterial chromosome publication-title: Methods Enzymol. doi: 10.1016/0076-6879(87)53055-5 contributor: fullname: Golden – volume: 578 start-page: 229 year: 2020 ident: 10.1016/j.ymben.2022.11.005_bib14 article-title: The promise and challenge of therapeutic genome editing publication-title: Nature doi: 10.1038/s41586-020-1978-5 contributor: fullname: Doudna – volume: 25 start-page: 2078 year: 2009 ident: 10.1016/j.ymben.2022.11.005_bib26 article-title: The sequence alignment/map format and SAMtools publication-title: Bioinformatics doi: 10.1093/bioinformatics/btp352 contributor: fullname: Li – volume: 3 start-page: 423 year: 2018 ident: 10.1016/j.ymben.2022.11.005_bib3 article-title: Deaminase-mediated multiplex genome editing in Escherichia coli publication-title: Nat. Microbiol. doi: 10.1038/s41564-017-0102-6 contributor: fullname: Banno – volume: 533 start-page: 420 year: 2016 ident: 10.1016/j.ymben.2022.11.005_bib23 article-title: Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage publication-title: Nature doi: 10.1038/nature17946 contributor: fullname: Komor – volume: 11 start-page: 1688 year: 2020 ident: 10.1016/j.ymben.2022.11.005_bib45 article-title: The circadian clock and darkness control natural competence in cyanobacteria publication-title: Nat. Commun. doi: 10.1038/s41467-020-15384-9 contributor: fullname: Taton – volume: 162 start-page: 2029 year: 2016 ident: 10.1016/j.ymben.2022.11.005_bib8 article-title: Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium Synechococcus elongatus PCC 7942 publication-title: Microbiology doi: 10.1099/mic.0.000377 contributor: fullname: Chen – volume: 551 start-page: 464 year: 2017 ident: 10.1016/j.ymben.2022.11.005_bib16 article-title: Programmable base editing of A⋅T to G⋅C in genomic DNA without DNA cleavage publication-title: Nature doi: 10.1038/nature24644 contributor: fullname: Gaudelli – year: 2022 ident: 10.1016/j.ymben.2022.11.005_bib1 article-title: Can microbes be harnessed to reduce atmospheric loads of greenhouse gases? publication-title: Environ. Microbiol. contributor: fullname: Ahn – volume: 12 start-page: 2765 year: 2019 ident: 10.1016/j.ymben.2022.11.005_bib31 article-title: Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2 in cyanobacteria publication-title: Energy Environ. Sci. doi: 10.1039/C9EE01214A contributor: fullname: Liu – volume: 24 year: 2021 ident: 10.1016/j.ymben.2022.11.005_bib17 article-title: Synthetic biology strategies to address waste CO2 loss during biofuel production publication-title: Curr. Opin. Environ. Sci. Health contributor: fullname: Godar – volume: 37 start-page: 771 year: 2019 ident: 10.1016/j.ymben.2022.11.005_bib33 article-title: Progress and perspective on cyanobacterial glycogen metabolism engineering publication-title: Biotechnol. Adv. doi: 10.1016/j.biotechadv.2019.04.005 contributor: fullname: Luan – volume: 33 start-page: 352 year: 2015 ident: 10.1016/j.ymben.2022.11.005_bib2 article-title: Metabolic engineering of cyanobacteria for the synthesis of commodity products publication-title: Trends Biotechnol. doi: 10.1016/j.tibtech.2015.03.009 contributor: fullname: Angermayr – volume: 10 year: 2022 ident: 10.1016/j.ymben.2022.11.005_bib57 article-title: Reprogramming microbial CO2-metabolizing chassis with CRISPR-Cas systems publication-title: Front. Bioeng. Biotechnol. doi: 10.3389/fbioe.2022.897204 contributor: fullname: Yu – volume: 362 start-page: 155 year: 2007 ident: 10.1016/j.ymben.2022.11.005_bib10 article-title: Specialized techniques for site-directed mutagenesis in cyanobacteria publication-title: Methods Mol. Biol. doi: 10.1007/978-1-59745-257-1_11 contributor: fullname: Clerico – volume: 7 year: 2017 ident: 10.1016/j.ymben.2022.11.005_bib5 article-title: AraBAD based toolkit for gene expression and metabolic robustness improvement in Synechococcus elongatus publication-title: Sci. Rep. doi: 10.1038/s41598-017-17035-4 contributor: fullname: Cao – volume: 115 start-page: E7805 year: 2018 ident: 10.1016/j.ymben.2022.11.005_bib11 article-title: Roles for ClpXP in regulating the circadian clock in Synechococcus elongatus publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.1800828115 contributor: fullname: Cohen – volume: 19 start-page: 770 year: 2018 ident: 10.1016/j.ymben.2022.11.005_bib40 article-title: Base editing: precision chemistry on the genome and transcriptome of living cells publication-title: Nat. Rev. Genet. doi: 10.1038/s41576-018-0059-1 contributor: fullname: Rees |
| SSID | ssj0011591 |
| Score | 2.461403 |
| Snippet | Cyanobacteria can directly convert carbon dioxide (CO2) at the atmospheric level to biofuels, value-added chemicals and food products, making them ideal... Cyanobacteria can directly convert carbon dioxide (CO ) at the atmospheric level to biofuels, value-added chemicals and food products, making them ideal... |
| SourceID | crossref pubmed elsevier |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 91 |
| SubjectTerms | Base editing Carbon Dioxide - metabolism CRISPR CRISPR-Cas Systems Cyanobacteria Gene Editing Metabolic Engineering Metabolic Networks and Pathways Multiplex Synechococcus - genetics Synechococcus - metabolism Synechococcus elongatus |
| Title | Base editing for reprogramming cyanobacterium Synechococcus elongatus |
| URI | https://dx.doi.org/10.1016/j.ymben.2022.11.005 https://www.ncbi.nlm.nih.gov/pubmed/36403709 |
| Volume | 75 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1NT8JAEJ0QjIkejOIXfpAePFrYLttte0QCQY1ckIRb0253DUYKkXLoxd_u7LYleOHgrWm2X6_bmTft6xuAB_0pTFFJbSqEazNMSLbPibID5nlcIMdnxq7pbcxHU_Yyc2c16Ff_wmhZZRn7i5huonW5plOi2VnN552Jg-wbixFOtSyI-drxk2H6wznd_tnKPJDwmK55erCtR1fOQ0bjlS9iqU1QKW1rK0_dw25vdtpJPcNTOCk5o9UrTusMajJtwGHRRTJvwPGOp-A5DJ4wL1l4WVrPbCEltbRvpRFhLfQakUcpPsPGo3mzsCZ5KjECYlgUm7Ulv5bpR5Rt1hcwHQ7e-yO77JVgC0ZYZmMCErGmA1jOEBVJkSiHJyx2AqZkwJirpOSJF5HAD1yaCIcSV7gxUViI4rZO9xLq6TKV12A5nvRirpSPC4zGJJCJUFhX-SRSAhlMEx4rjMJVYYkRVlqxz9BAGmpIsbgIEdIm8ArH8M-dDTFo79_wqkB9e5QuZ6TrkeDmv7u8hSPdLr54hXIH9ex7I--RVGRxy8yaFhz0nl9H418pjMnY |
| link.rule.ids | 315,783,787,4509,24128,27936,27937,45597,45691 |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT8JAEJ4gxqgHo_jCZw8eLWy32217VAJBBS5Awq1pt7sGI4UIHHrxtzvbB8GLB29Ns3193c580379BuBBfwpTVFKTCuGYDBOS6XGiTJ-5LhfI8Vlm19Qf8O6YvU6cSQVa5b8wWlZZxP48pmfRuljTLNBsLqbT5tBC9o3FCKdaFsQ8ewd2mebHOKkb3xudBzKerG2eHm3q4aX1UCbySmeR1C6olDa0l6duYvdnetrKPZ1jOCpIo_GUn9cJVGRSg728jWRag8MtU8FTaD9jYjLwurSg2UBOamjjykyFNdNrRBom-BBnJs3rmTFME4khEOOiWC8N-TlP3sPVenkG40571OqaRbMEUzDCViZmIBFpPoD1DFGhFLGyeMwiy2dK-ow5SkoeuyHxPd-hsbAocYQTEYWVKG5r2edQTeaJvATDcqUbcaU8XGA0Ir6MhcLCyiOhEkhh6vBYYhQsck-MoBSLfQQZpIGGFKuLACGtAy9xDH7d2gCj9t8bXuSob45ic0Zsl_hX_93lPex3R_1e0HsZvF3Dge4dn79PuYHq6mstb5FhrKK7bAb9ABUky3E |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Base+editing+for+reprogramming+cyanobacterium+Synechococcus+elongatus&rft.jtitle=Metabolic+engineering&rft.au=Wang%2C+Shu-Yan&rft.au=Li%2C+Xin&rft.au=Wang%2C+Shu-Guang&rft.au=Xia%2C+Peng-Fei&rft.date=2023-01-01&rft.issn=1096-7176&rft.volume=75&rft.spage=91&rft.epage=99&rft_id=info:doi/10.1016%2Fj.ymben.2022.11.005&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_j_ymben_2022_11_005 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1096-7176&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1096-7176&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1096-7176&client=summon |