NK-KIR ligand identification: a quick Q-PCR approach for HLA-C epitope typing
Interaction of donor natural killer (NK)‐cell‐associated killer cell immunoglobulin‐like receptors (KIRs) with the patient’s human leukocyte antigen‐C (HLA‐C) ligands can result in an alloreactive NK response after haematopoietic stem cell transplantation. In many retrospective studies, additional H...
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| Published in | Tissue antigens Vol. 69; no. 4; pp. 334 - 337 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
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Oxford, UK
Blackwell Publishing Ltd
01.04.2007
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| Abstract | Interaction of donor natural killer (NK)‐cell‐associated killer cell immunoglobulin‐like receptors (KIRs) with the patient’s human leukocyte antigen‐C (HLA‐C) ligands can result in an alloreactive NK response after haematopoietic stem cell transplantation. In many retrospective studies, additional HLA‐C‐typing data are required to predict NK‐cell alloreactivity. We developed a Taqman assay using the quantitative polymerase chain reaction (Q‐PCR) technique that facilitates HLA‐C epitope typing, allowing the assignment of HLA‐C group 1 or 2 alleles based on the dimorphism at residues 77 and 80 rather than based on the sequence specific priming (SSP) and sequence‐based typing allele types. Q‐PCR analysis for HLA‐C epitope detection showed three clusters reflecting homozygous group 1 or 2 and heterozygous samples. This new approach introduces a quick HLA‐C epitope screening method to define the presence of the ligand for the KIR–HLA‐C interaction. |
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| AbstractList | Interaction of donor natural killer (NK)-cell-associated killer cell immunoglobulin-like receptors (KIRs) with the patient's human leukocyte antigen-C (HLA-C) ligands can result in an alloreactive NK response after haematopoietic stem cell transplantation. In many retrospective studies, additional HLA-C-typing data are required to predict NK-cell alloreactivity. We developed a Taqman assay using the quantitative polymerase chain reaction (Q-PCR) technique that facilitates HLA-C epitope typing, allowing the assignment of HLA-C group 1 or 2 alleles based on the dimorphism at residues 77 and 80 rather than based on the sequence specific priming (SSP) and sequence-based typing allele types. Q-PCR analysis for HLA-C epitope detection showed three clusters reflecting homozygous group 1 or 2 and heterozygous samples. This new approach introduces a quick HLA-C epitope screening method to define the presence of the ligand for the KIR-HLA-C interaction. Abstract Interaction of donor natural killer (NK)‐cell‐associated killer cell immunoglobulin‐like receptors (KIRs) with the patient’s human leukocyte antigen‐C (HLA‐C) ligands can result in an alloreactive NK response after haematopoietic stem cell transplantation. In many retrospective studies, additional HLA‐C‐typing data are required to predict NK‐cell alloreactivity. We developed a Taqman assay using the quantitative polymerase chain reaction (Q‐PCR) technique that facilitates HLA‐C epitope typing, allowing the assignment of HLA‐C group 1 or 2 alleles based on the dimorphism at residues 77 and 80 rather than based on the sequence specific priming (SSP) and sequence‐based typing allele types. Q‐PCR analysis for HLA‐C epitope detection showed three clusters reflecting homozygous group 1 or 2 and heterozygous samples. This new approach introduces a quick HLA‐C epitope screening method to define the presence of the ligand for the KIR–HLA‐C interaction. |
| Author | Borst, H. P. E. Tilanus, M. G. J. Otten, H. G. Van Den Tweel, J. G. Rozemuller, E. H. Schellekens, J. |
| Author_xml | – sequence: 1 givenname: J. surname: Schellekens fullname: Schellekens, J. organization: Department of Pathology, University Medical Centre Utrecht, Utrecht, The Netherlands – sequence: 2 givenname: E. H. surname: Rozemuller fullname: Rozemuller, E. H. organization: Department of Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands – sequence: 3 givenname: H. P. E. surname: Borst fullname: Borst, H. P. E. organization: Department of Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands – sequence: 4 givenname: H. G. surname: Otten fullname: Otten, H. G. organization: Department of Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands – sequence: 5 givenname: J. G. surname: Van Den Tweel fullname: Van Den Tweel, J. G. organization: Department of Pathology, University Medical Centre Utrecht, Utrecht, The Netherlands – sequence: 6 givenname: M. G. J. surname: Tilanus fullname: Tilanus, M. G. J. email: m.tilanus@umcutrecht.nl organization: Department of Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands |
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| Cites_doi | 10.1038/nri999 10.1182/asheducation-2003.1.350 10.1111/j.1399-0039.1998.tb03087.x 10.1038/nri1570 10.1182/blood.V75.3.555.555 10.1200/JCO.1997.15.2.433 10.1016/S0198-8859(01)00298-1 10.1126/science.8493555 10.1182/blood-2002-02-0350 10.1146/annurev.immunol.20.092501.134942 10.1146/annurev.immunol.23.021704.115526 10.1126/science.1068440 10.1084/jem.182.2.605 10.1093/nar/16.3.1215 10.1111/j.1399-0039.1998.tb03092.x 10.1111/j.1399-0039.2004.00188.x 10.1073/pnas.90.24.12000 |
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| References_xml | – volume: 52 start-page: 558 year: 1998 end-page: 68 article-title: A reliable and efficient high resolution typing method for HLA‐C using sequence‐based typing publication-title: Tissue Antigens – volume: 260 start-page: 1121 year: 1993 end-page: 4 article-title: Generation of allospecific natural killer cells by stimulation across a polymorphism of HLA‐C publication-title: Science – volume: 5 start-page: 201 year: 2005 end-page: 14 article-title: MHC class I molecules and KIRs in human history, health and survival publication-title: Nat Rev Immunol – volume: 63 start-page: 101 year: 2004 end-page: 8 article-title: The structural basis of T‐cell allorecognition publication-title: Tissue Antigens – volume: 62 start-page: 1009 year: 2001 end-page: 30 article-title: Analysis of the frequencies of HLA‐A, B, and C alleles and haplotypes in the five major ethnic groups of the United States reveals high levels of diversity in these loci and contrasting distribution patterns in these populations publication-title: Hum Immunol – volume: 15 start-page: 433 year: 1997 end-page: 44 article-title: Donor leukocyte infusions in 140 patients with relapsed malignancy after allogeneic bone marrow transplantation publication-title: J Clin Oncol – volume: 20 start-page: 217 year: 2002 end-page: 51 article-title: KIR: diverse, rapidly evolving receptors of innate and adaptive immunity publication-title: Annu Rev Immunol – volume: 100 start-page: 1935 year: 2002 end-page: 47 article-title: Natural killer cell receptors: new biology and insights into the graft‐versus‐leukemia effect publication-title: Blood – volume: 16 start-page: 1215 year: 1988 article-title: A simple salting out procedure for extracting DNA from human nucleated cells publication-title: Nucleic Acids Res – volume: 90 start-page: 12000 year: 1993 end-page: 4 article-title: HLA‐C is the inhibitory ligand that determines dominant resistance to lysis by NK1‐ and NK2‐specific natural killer cells publication-title: Proc Natl Acad Sci USA – volume: 23 start-page: 225 year: 2005 end-page: 74 article-title: NK cell recognition publication-title: Annu Rev Immunol – volume: 52 start-page: 587 year: 1998 end-page: 9 article-title: Identification of an HLA‐C variant allele, CW*0805, by sequencing based typing publication-title: Tissue Antigens – volume: 182 start-page: 605 year: 1995 end-page: 9 article-title: Amino acid substitutions can influence the natural killer (NK)‐mediated recognition of HLA‐C molecules. Role of serine‐77 and lysine‐80 in the target cell protection from lysis mediated by “group 2” or “group 1” NK clones publication-title: J Exp Med – volume: 3 start-page: 108 year: 2003 end-page: 22 article-title: Alloreactive killer cells: hindrance and help for haematopoietic transplants publication-title: Nat Rev Immunol – volume: 75 start-page: 555 year: 1990 end-page: 62 article-title: Graft‐versus‐leukemia reactions after bone marrow transplantation publication-title: Blood – start-page: 350 year: 2003 end-page: 71 article-title: New developments in allotransplant immunology publication-title: Hematology – volume: 295 start-page: 2097 year: 2002 end-page: 100 article-title: Effectiveness of donor natural killer cell alloreactivity in mismatched hematopoietic transplants publication-title: Science – ident: e_1_2_3_8_2 doi: 10.1038/nri999 – ident: e_1_2_3_4_2 doi: 10.1182/asheducation-2003.1.350 – ident: e_1_2_3_17_2 doi: 10.1111/j.1399-0039.1998.tb03087.x – ident: e_1_2_3_7_2 doi: 10.1038/nri1570 – ident: e_1_2_3_2_2 doi: 10.1182/blood.V75.3.555.555 – ident: e_1_2_3_3_2 doi: 10.1200/JCO.1997.15.2.433 – ident: e_1_2_3_18_2 doi: 10.1016/S0198-8859(01)00298-1 – ident: e_1_2_3_13_2 doi: 10.1126/science.8493555 – ident: e_1_2_3_14_2 doi: 10.1182/blood-2002-02-0350 – ident: e_1_2_3_9_2 doi: 10.1146/annurev.immunol.20.092501.134942 – ident: e_1_2_3_10_2 doi: 10.1146/annurev.immunol.23.021704.115526 – ident: e_1_2_3_6_2 doi: 10.1126/science.1068440 – ident: e_1_2_3_11_2 doi: 10.1084/jem.182.2.605 – ident: e_1_2_3_15_2 doi: 10.1093/nar/16.3.1215 – ident: e_1_2_3_16_2 doi: 10.1111/j.1399-0039.1998.tb03092.x – ident: e_1_2_3_5_2 doi: 10.1111/j.1399-0039.2004.00188.x – ident: e_1_2_3_12_2 doi: 10.1073/pnas.90.24.12000 |
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| Snippet | Interaction of donor natural killer (NK)‐cell‐associated killer cell immunoglobulin‐like receptors (KIRs) with the patient’s human leukocyte antigen‐C (HLA‐C)... Interaction of donor natural killer (NK)-cell-associated killer cell immunoglobulin-like receptors (KIRs) with the patient's human leukocyte antigen-C (HLA-C)... Abstract Interaction of donor natural killer (NK)‐cell‐associated killer cell immunoglobulin‐like receptors (KIRs) with the patient’s human leukocyte antigen‐C... |
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| SubjectTerms | Alleles DNA Primers - chemistry Epitopes - genetics Histocompatibility Histocompatibility Testing - methods HLA Antigens - genetics HLA-C Antigens - biosynthesis HLA-C Antigens - genetics HLA-C epitope Homozygote Humans Killer Cells, Natural - cytology KIR KIR ligand Ligands Polymerase Chain Reaction - methods Q-PCR |
| Title | NK-KIR ligand identification: a quick Q-PCR approach for HLA-C epitope typing |
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