Comparative electron microscopic visualization of the lung alveolar epithelial glycocalyx with different staining and labeling methods
The alveolar surface of the lung is lined by an epithelium consisting of type I (AECI) and type II alveolar epithelial cells (AECII). This epithelium is covered by a liquid alveolar lining layer (ALL). Besides intra‐alveolar surfactant, ALL also contains the alveolar epithelial glycocalyx on the api...
Saved in:
| Published in | Journal of anatomy Vol. 246; no. 5; pp. 770 - 781 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Wiley Subscription Services, Inc
01.05.2025
John Wiley and Sons Inc |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0021-8782 1469-7580 1469-7580 |
| DOI | 10.1111/joa.14129 |
Cover
| Abstract | The alveolar surface of the lung is lined by an epithelium consisting of type I (AECI) and type II alveolar epithelial cells (AECII). This epithelium is covered by a liquid alveolar lining layer (ALL). Besides intra‐alveolar surfactant, ALL also contains the alveolar epithelial glycocalyx on the apical side of AECI and AECII. To better understand the alveolar epithelial glycocalyx, its ultrastructural visualization by transmission electron microscopy is required. The aim of this study was to systematically re‐evaluate routine cytochemical methods for visualization of the alveolar epithelial glycocalyx and specifically its glycan components. For this purpose, we used chemical fixation by vascular perfusion with aldehydes as a common routine approach in mice. After fixation, staining is needed for glycocalyx visualization. Cytochemical staining agents such as alcian blue, ruthenium red, and lanthanum nitrate were compared. In addition, SNL (Sambucus nigra lectin) and UEA1 (Ulex europaeus agglutinin I) were used for sialic acid and fucose‐specific labeling. Alcian blue showed the strongest staining, with cloud‐like structures, whereas ruthenium red appeared as thread‐like structures. On the other hand, lanthanum nitrate did not stain the alveolar epithelial glycocalyx. For specific sialic acid and fucose labeling, both lectins presented a specific signal. In conclusion, these methods can be used routinely for assessing ultrastructural changes of the alveolar epithelial glycocalyx in experimental in vivo models under different physiological and pathological conditions. In addition, cytochemical staining by tissue massage and post‐embedding lectin labeling after vascular perfusion support 3R (reduction, refinement, replacement) principles of animal welfare.
Cytochemical staining by tissue massage and post‐embedding lectin labeling after vascular perfusion support 3R principles of animal welfare, by reducing the number of animals. These methods can be used routinely for assessing ultrastructural changes of the alveolar epithelial glycocalyx in experimental in vivo models under different physiological and pathological conditions. |
|---|---|
| AbstractList | The alveolar surface of the lung is lined by an epithelium consisting of type I (AECI) and type II alveolar epithelial cells (AECII). This epithelium is covered by a liquid alveolar lining layer (ALL). Besides intra‐alveolar surfactant, ALL also contains the alveolar epithelial glycocalyx on the apical side of AECI and AECII. To better understand the alveolar epithelial glycocalyx, its ultrastructural visualization by transmission electron microscopy is required. The aim of this study was to systematically re‐evaluate routine cytochemical methods for visualization of the alveolar epithelial glycocalyx and specifically its glycan components. For this purpose, we used chemical fixation by vascular perfusion with aldehydes as a common routine approach in mice. After fixation, staining is needed for glycocalyx visualization. Cytochemical staining agents such as alcian blue, ruthenium red, and lanthanum nitrate were compared. In addition, SNL ( Sambucus nigra lectin) and UEA1 ( Ulex europaeus agglutinin I) were used for sialic acid and fucose‐specific labeling. Alcian blue showed the strongest staining, with cloud‐like structures, whereas ruthenium red appeared as thread‐like structures. On the other hand, lanthanum nitrate did not stain the alveolar epithelial glycocalyx. For specific sialic acid and fucose labeling, both lectins presented a specific signal. In conclusion, these methods can be used routinely for assessing ultrastructural changes of the alveolar epithelial glycocalyx in experimental in vivo models under different physiological and pathological conditions. In addition, cytochemical staining by tissue massage and post‐embedding lectin labeling after vascular perfusion support 3R (reduction, refinement, replacement) principles of animal welfare. The alveolar surface of the lung is lined by an epithelium consisting of type I (AECI) and type II alveolar epithelial cells (AECII). This epithelium is covered by a liquid alveolar lining layer (ALL). Besides intra‐alveolar surfactant, ALL also contains the alveolar epithelial glycocalyx on the apical side of AECI and AECII. To better understand the alveolar epithelial glycocalyx, its ultrastructural visualization by transmission electron microscopy is required. The aim of this study was to systematically re‐evaluate routine cytochemical methods for visualization of the alveolar epithelial glycocalyx and specifically its glycan components. For this purpose, we used chemical fixation by vascular perfusion with aldehydes as a common routine approach in mice. After fixation, staining is needed for glycocalyx visualization. Cytochemical staining agents such as alcian blue, ruthenium red, and lanthanum nitrate were compared. In addition, SNL (Sambucus nigra lectin) and UEA1 (Ulex europaeus agglutinin I) were used for sialic acid and fucose‐specific labeling. Alcian blue showed the strongest staining, with cloud‐like structures, whereas ruthenium red appeared as thread‐like structures. On the other hand, lanthanum nitrate did not stain the alveolar epithelial glycocalyx. For specific sialic acid and fucose labeling, both lectins presented a specific signal. In conclusion, these methods can be used routinely for assessing ultrastructural changes of the alveolar epithelial glycocalyx in experimental in vivo models under different physiological and pathological conditions. In addition, cytochemical staining by tissue massage and post‐embedding lectin labeling after vascular perfusion support 3R (reduction, refinement, replacement) principles of animal welfare. Cytochemical staining by tissue massage and post‐embedding lectin labeling after vascular perfusion support 3R principles of animal welfare, by reducing the number of animals. These methods can be used routinely for assessing ultrastructural changes of the alveolar epithelial glycocalyx in experimental in vivo models under different physiological and pathological conditions. The alveolar surface of the lung is lined by an epithelium consisting of type I (AECI) and type II alveolar epithelial cells (AECII). This epithelium is covered by a liquid alveolar lining layer (ALL). Besides intra-alveolar surfactant, ALL also contains the alveolar epithelial glycocalyx on the apical side of AECI and AECII. To better understand the alveolar epithelial glycocalyx, its ultrastructural visualization by transmission electron microscopy is required. The aim of this study was to systematically re-evaluate routine cytochemical methods for visualization of the alveolar epithelial glycocalyx and specifically its glycan components. For this purpose, we used chemical fixation by vascular perfusion with aldehydes as a common routine approach in mice. After fixation, staining is needed for glycocalyx visualization. Cytochemical staining agents such as alcian blue, ruthenium red, and lanthanum nitrate were compared. In addition, SNL (Sambucus nigra lectin) and UEA1 (Ulex europaeus agglutinin I) were used for sialic acid and fucose-specific labeling. Alcian blue showed the strongest staining, with cloud-like structures, whereas ruthenium red appeared as thread-like structures. On the other hand, lanthanum nitrate did not stain the alveolar epithelial glycocalyx. For specific sialic acid and fucose labeling, both lectins presented a specific signal. In conclusion, these methods can be used routinely for assessing ultrastructural changes of the alveolar epithelial glycocalyx in experimental in vivo models under different physiological and pathological conditions. In addition, cytochemical staining by tissue massage and post-embedding lectin labeling after vascular perfusion support 3R (reduction, refinement, replacement) principles of animal welfare.The alveolar surface of the lung is lined by an epithelium consisting of type I (AECI) and type II alveolar epithelial cells (AECII). This epithelium is covered by a liquid alveolar lining layer (ALL). Besides intra-alveolar surfactant, ALL also contains the alveolar epithelial glycocalyx on the apical side of AECI and AECII. To better understand the alveolar epithelial glycocalyx, its ultrastructural visualization by transmission electron microscopy is required. The aim of this study was to systematically re-evaluate routine cytochemical methods for visualization of the alveolar epithelial glycocalyx and specifically its glycan components. For this purpose, we used chemical fixation by vascular perfusion with aldehydes as a common routine approach in mice. After fixation, staining is needed for glycocalyx visualization. Cytochemical staining agents such as alcian blue, ruthenium red, and lanthanum nitrate were compared. In addition, SNL (Sambucus nigra lectin) and UEA1 (Ulex europaeus agglutinin I) were used for sialic acid and fucose-specific labeling. Alcian blue showed the strongest staining, with cloud-like structures, whereas ruthenium red appeared as thread-like structures. On the other hand, lanthanum nitrate did not stain the alveolar epithelial glycocalyx. For specific sialic acid and fucose labeling, both lectins presented a specific signal. In conclusion, these methods can be used routinely for assessing ultrastructural changes of the alveolar epithelial glycocalyx in experimental in vivo models under different physiological and pathological conditions. In addition, cytochemical staining by tissue massage and post-embedding lectin labeling after vascular perfusion support 3R (reduction, refinement, replacement) principles of animal welfare. The alveolar surface of the lung is lined by an epithelium consisting of type I (AECI) and type II alveolar epithelial cells (AECII). This epithelium is covered by a liquid alveolar lining layer (ALL). Besides intra‐alveolar surfactant, ALL also contains the alveolar epithelial glycocalyx on the apical side of AECI and AECII. To better understand the alveolar epithelial glycocalyx, its ultrastructural visualization by transmission electron microscopy is required. The aim of this study was to systematically re‐evaluate routine cytochemical methods for visualization of the alveolar epithelial glycocalyx and specifically its glycan components. For this purpose, we used chemical fixation by vascular perfusion with aldehydes as a common routine approach in mice. After fixation, staining is needed for glycocalyx visualization. Cytochemical staining agents such as alcian blue, ruthenium red, and lanthanum nitrate were compared. In addition, SNL (Sambucus nigra lectin) and UEA1 (Ulex europaeus agglutinin I) were used for sialic acid and fucose‐specific labeling. Alcian blue showed the strongest staining, with cloud‐like structures, whereas ruthenium red appeared as thread‐like structures. On the other hand, lanthanum nitrate did not stain the alveolar epithelial glycocalyx. For specific sialic acid and fucose labeling, both lectins presented a specific signal. In conclusion, these methods can be used routinely for assessing ultrastructural changes of the alveolar epithelial glycocalyx in experimental in vivo models under different physiological and pathological conditions. In addition, cytochemical staining by tissue massage and post‐embedding lectin labeling after vascular perfusion support 3R (reduction, refinement, replacement) principles of animal welfare. The alveolar surface of the lung is lined by an epithelium consisting of type I (AECI) and type II alveolar epithelial cells (AECII). This epithelium is covered by a liquid alveolar lining layer (ALL). Besides intra‐alveolar surfactant, ALL also contains the alveolar epithelial glycocalyx on the apical side of AECI and AECII. To better understand the alveolar epithelial glycocalyx, its ultrastructural visualization by transmission electron microscopy is required. The aim of this study was to systematically re‐evaluate routine cytochemical methods for visualization of the alveolar epithelial glycocalyx and specifically its glycan components. For this purpose, we used chemical fixation by vascular perfusion with aldehydes as a common routine approach in mice. After fixation, staining is needed for glycocalyx visualization. Cytochemical staining agents such as alcian blue, ruthenium red, and lanthanum nitrate were compared. In addition, SNL ( Sambucus nigra lectin) and UEA1 ( Ulex europaeus agglutinin I) were used for sialic acid and fucose‐specific labeling. Alcian blue showed the strongest staining, with cloud‐like structures, whereas ruthenium red appeared as thread‐like structures. On the other hand, lanthanum nitrate did not stain the alveolar epithelial glycocalyx. For specific sialic acid and fucose labeling, both lectins presented a specific signal. In conclusion, these methods can be used routinely for assessing ultrastructural changes of the alveolar epithelial glycocalyx in experimental in vivo models under different physiological and pathological conditions. In addition, cytochemical staining by tissue massage and post‐embedding lectin labeling after vascular perfusion support 3R (reduction, refinement, replacement) principles of animal welfare. Cytochemical staining by tissue massage and post‐embedding lectin labeling after vascular perfusion support 3R principles of animal welfare, by reducing the number of animals. These methods can be used routinely for assessing ultrastructural changes of the alveolar epithelial glycocalyx in experimental in vivo models under different physiological and pathological conditions. |
| Author | Kuebler, Wolfgang M. Ochs, Matthias Gluhovic, Vladimir Lopez‐Rodriguez, Elena Timm, Sara |
| AuthorAffiliation | 4 German Center for Cardiovascular Research (DZHK) Berlin Germany 2 Core Facility Electron Microscopy Charité—Universitätsmedizin Berlin Berlin Germany 7 German Center for Lung Research (DZL) Berlin Germany 5 Keenan Research Centre St. Michael's Hospital, University of Toronto Toronto Ontario Canada 6 Department of Surgery and Physiology University of Toronto Toronto Ontario Canada 1 Institute of Functional Anatomy, Charité—Universitätsmedizin Berlin Berlin Germany 3 Institute of Physiology, Charité—Universitätsmedizin Berlin Berlin Germany |
| AuthorAffiliation_xml | – name: 6 Department of Surgery and Physiology University of Toronto Toronto Ontario Canada – name: 1 Institute of Functional Anatomy, Charité—Universitätsmedizin Berlin Berlin Germany – name: 4 German Center for Cardiovascular Research (DZHK) Berlin Germany – name: 7 German Center for Lung Research (DZL) Berlin Germany – name: 5 Keenan Research Centre St. Michael's Hospital, University of Toronto Toronto Ontario Canada – name: 2 Core Facility Electron Microscopy Charité—Universitätsmedizin Berlin Berlin Germany – name: 3 Institute of Physiology, Charité—Universitätsmedizin Berlin Berlin Germany |
| Author_xml | – sequence: 1 givenname: Vladimir orcidid: 0009-0002-6807-4033 surname: Gluhovic fullname: Gluhovic, Vladimir email: vladimir.gluhovic@charite.de organization: Institute of Functional Anatomy, Charité—Universitätsmedizin Berlin – sequence: 2 givenname: Sara surname: Timm fullname: Timm, Sara organization: Charité—Universitätsmedizin Berlin – sequence: 3 givenname: Wolfgang M. surname: Kuebler fullname: Kuebler, Wolfgang M. organization: German Center for Lung Research (DZL) – sequence: 4 givenname: Elena surname: Lopez‐Rodriguez fullname: Lopez‐Rodriguez, Elena organization: Institute of Functional Anatomy, Charité—Universitätsmedizin Berlin – sequence: 5 givenname: Matthias surname: Ochs fullname: Ochs, Matthias organization: German Center for Lung Research (DZL) |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/39245632$$D View this record in MEDLINE/PubMed |
| BookMark | eNp1kU1v1DAQhi1URLeFA38AWeICh7R2nC-fULXiU5V6gbM1cSa7Xjl2sJMtyw_o767DlgqQ8GXkmWdevaP3jJw475CQl5xd8PQudx4ueMFz-YSseFHJrC4bdkJWjOU8a-omPyVnMe4Y44LJ4hk5FTIvykrkK3K39sMIASazR4oW9RS8o4PRwUftR6Pp3sQZrPmZkDTxPZ22SO3sNhTsHr2FQHE0qWkNWLqxB-012MMPepuatDN9jwHdROMExpllzXXUQpv49Blw2vouPidPe7ARXzzUc_Ltw_uv60_Z9c3Hz-ur60wXrJCZ7qQUXdHppmId9oBQd-kkyFkjddvXdVk2XDQtQxAoBPS8QsGqJucceduiOCfvjrrj3A7Y6WQsgFVjMAOEg_Jg1N8TZ7Zq4_eKcymrmhVJ4c2DQvDfZ4yTGkzUaC049HNUgrOc1YznC_r6H3Tn5-DSfYmSSxqylIl69aelRy-_M0rA2yOwZBID9o8IZ2rJP8mC-pV_Yi-P7K2xePg_qL7cXB037gEvi7UE |
| Cites_doi | 10.1007/s00424‐007‐0212‐8 10.1007/s00418‐023‐02211‐7 10.1152/jappl.1995.79.5.1615 10.1177/35.9.3302020 10.1016/j.bbamem.2008.05.003 10.1152/ajpcell.00555.2022 10.1083/jcb.17.1.208 10.1002/ar.1091710302 10.3389/fcell.2020.00253 10.1177/30.12.6185564 10.1165/rcmb.2017‐0428OC 10.5694/j.1326‐5377.1960.tb73127.x 10.1097/00004032‐198306001‐00022 10.1083/jcb.25.1.23 10.1177/38.2.1688898 10.1111/joim.12465 10.1152/physrev.1973.53.2.419 10.1177/31.8.6190857 10.1083/jcb.51.3.611 10.1007/BF02621963 10.1016/s0074‐7696(02)16005‐0 10.1016/0034‐5687(75)90083‐3 10.1159/000272061 10.1038/1751125b0 10.1186/1471‐2180‐14‐78 10.1002/ar.1091670403 10.1016/0034‐5687(68)90006‐6 10.1001/archinte.1971.00310170104014 10.1016/0034‐5687(69)90042‐5 10.1016/j.biopha.2020.111026 10.1007/978-3-0348-8855-4_17 10.1007/s11897‐017‐0320‐5 10.1007/s00418‐018‐1747‐9 10.1007/BF01005264 10.3389/fimmu.2021.693149 10.1177/11.1.14 10.3390/ijms21093075 10.1007/s00418‐016‐1502‐z 10.1007/s00289‐012‐0853‐4 10.1016/j.morpho.2017.04.001 10.1016/0378‐5955(87)90025‐6 10.1172/jci.insight.154573 10.1016/j.jcf.2004.05.021 10.1017/S1431927621011958 10.1007/BF00306149 10.1007/978-1-4614-7520-0_7 10.1016/B978-0-7020-6864-5.00013-X 10.1007/s00418‐010‐0736‐4 |
| ContentType | Journal Article |
| Copyright | 2024 The Author(s). published by John Wiley & Sons Ltd on behalf of Anatomical Society. 2024 The Author(s). Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society. 2024. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| Copyright_xml | – notice: 2024 The Author(s). published by John Wiley & Sons Ltd on behalf of Anatomical Society. – notice: 2024 The Author(s). Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society. – notice: 2024. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| DBID | 24P AAYXX CITATION CGR CUY CVF ECM EIF NPM 7QP 7QR 7SS 7TK 8FD FR3 K9. P64 7X8 5PM |
| DOI | 10.1111/joa.14129 |
| DatabaseName | Wiley Online Library Open Access CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Entomology Abstracts (Full archive) Neurosciences Abstracts Technology Research Database Engineering Research Database ProQuest Health & Medical Complete (Alumni) Biotechnology and BioEngineering Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Entomology Abstracts Technology Research Database ProQuest Health & Medical Complete (Alumni) Chemoreception Abstracts Engineering Research Database Calcium & Calcified Tissue Abstracts Neurosciences Abstracts Biotechnology and BioEngineering Abstracts MEDLINE - Academic |
| DatabaseTitleList | CrossRef MEDLINE MEDLINE - Academic Entomology Abstracts |
| Database_xml | – sequence: 1 dbid: 24P name: Wiley Online Library Open Access (Activated by CARLI) url: https://authorservices.wiley.com/open-science/open-access/browse-journals.html sourceTypes: Publisher – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine Zoology Biology |
| DocumentTitleAlternate | Gluhovic et al |
| EISSN | 1469-7580 |
| EndPage | 781 |
| ExternalDocumentID | PMC11996704 39245632 10_1111_joa_14129 JOA14129 |
| Genre | researchArticle Research Support, Non-U.S. Gov't Journal Article Comparative Study |
| GrantInformation_xml | – fundername: Deutsche Forschungsgemeinschaft funderid: SFB 1449 ‐ 431232613 – fundername: Deutsche Forschungsgemeinschaft grantid: SFB 1449 - 431232613 |
| GroupedDBID | --- -~X .3N .55 .GA .GJ .Y3 05W 0R~ 10A 1OC 24P 29J 2WC 31~ 33P 36B 3O- 3SF 4.4 50Y 50Z 51W 51X 52M 52N 52O 52P 52R 52S 52T 52U 52V 52W 52X 53G 5GY 5HH 5LA 5VS 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A01 A03 AAESR AAEVG AAHQN AAIPD AAMMB AAMNL AANLZ AAONW AASGY AAXRX AAYCA AAZKR ABCQN ABCUV ABEML ABGDZ ABJNI ABLJU ABPVW ABQWH ABVKB ABXGK ACAHQ ACCZN ACFBH ACGFO ACGFS ACGOF ACIWK ACMXC ACNCT ACPOU ACPRK ACQPF ACSCC ACXBN ACXQS ADBBV ADBTR ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZMN AEFGJ AEGXH AEIGN AEIMD AENEX AEUYR AEYWJ AFBPY AFEBI AFFPM AFGKR AFWVQ AFZJQ AGHNM AGXDD AGYGG AHBTC AIACR AIAGR AIDQK AIDYY AITYG AIURR ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN ALVPJ AMBMR AMYDB AOIJS ATUGU AZBYB AZVAB BAFTC BAWUL BFHJK BHBCM BMXJE BROTX BRXPI BY8 C45 CAG COF CS3 D-6 D-7 D-E D-F DCZOG DIK DPXWK DR2 DRFUL DRMAN DRSTM DU5 E3Z EBS EJD EMOBN EX3 F00 F01 F04 F5P FIJ FUBAC G-S G.N GODZA GX1 H.X HF~ HGLYW HYE HZI HZ~ H~9 IHE IX1 J0M K48 KBYEO LATKE LC2 LC3 LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MK4 MRFUL MRMAN MRSTM MSFUL MSMAN MSSTM MXFUL MXMAN MXSTM N04 N05 N9A NF~ O66 O9- OBS OHT OIG OK1 OVD P2P P2W P2X P2Z P4B P4D Q.N Q11 QB0 R.K RCA RIG ROL RPM RX1 SUPJJ TEORI TR2 UB1 V8K W8V W99 WBKPD WH7 WHG WIH WIJ WIK WIN WNSPC WOHZO WOQ WOW WQJ WXI WXSBR WYISQ X7M XG1 XOL YFH YUY ZGI ZXP ZZTAW ~02 ~IA ~WT AAHHS AAYXX ACCFJ ADZOD AEEZP AEQDE AIWBW AJBDE CITATION CGR CUY CVF ECM EIF NPM 7QP 7QR 7SS 7TK 8FD FR3 K9. P64 7X8 5PM |
| ID | FETCH-LOGICAL-c4049-cd993d4dc860defaea7d309a2089cbf77558138b0ea3e33af16e3068211e1bbe3 |
| IEDL.DBID | DR2 |
| ISSN | 0021-8782 1469-7580 |
| IngestDate | Thu Aug 21 18:30:54 EDT 2025 Fri Sep 05 08:39:51 EDT 2025 Wed Aug 13 03:35:08 EDT 2025 Wed Jul 23 01:46:49 EDT 2025 Tue Jul 01 05:09:41 EDT 2025 Sun Jul 06 04:45:26 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 5 |
| Keywords | alveolar epithelial glycocalyx transmission electron microscopy alcian blue sialic acid lectin |
| Language | English |
| License | Attribution-NonCommercial-NoDerivs 2024 The Author(s). Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c4049-cd993d4dc860defaea7d309a2089cbf77558138b0ea3e33af16e3068211e1bbe3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| ORCID | 0009-0002-6807-4033 |
| OpenAccessLink | https://proxy.k.utb.cz/login?url=https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fjoa.14129 |
| PMID | 39245632 |
| PQID | 3190013959 |
| PQPubID | 1086345 |
| PageCount | 12 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_11996704 proquest_miscellaneous_3102070124 proquest_journals_3190013959 pubmed_primary_39245632 crossref_primary_10_1111_joa_14129 wiley_primary_10_1111_joa_14129_JOA14129 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | May 2025 |
| PublicationDateYYYYMMDD | 2025-05-01 |
| PublicationDate_xml | – month: 05 year: 2025 text: May 2025 |
| PublicationDecade | 2020 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England – name: Oxford – name: Hoboken |
| PublicationTitle | Journal of anatomy |
| PublicationTitleAlternate | J Anat |
| PublicationYear | 2025 |
| Publisher | Wiley Subscription Services, Inc John Wiley and Sons Inc |
| Publisher_xml | – name: Wiley Subscription Services, Inc – name: John Wiley and Sons Inc |
| References | 1987; 35 2021; 27 1955; 175 1970; 167 1973; 53 1995; 79 1968; 4 1964; 4 1960; 1 1971; 127 2016; 146 2004; 3 2013; 70 2020; 8 1969; 8 2007; 454 2010; 25 1971; 51 1965; 25 2014; 14 1971; 171 2023; 160 1990; 38 1982; 30 2015; 54 1998 2002; 216 1983; 31 1996 2023; 324 1964; 23 1972; 4 2008; 1778 2016; 280 1929; 66 1963; 11 2021; 12 2018; 150 2017; 14 2020 2022; 7 1984; 139 2010; 134 2019 1975; 23 2021; 133 2020; 21 2017; 101 1963; 17 2018; 59 1983; 44 1987; 27 e_1_2_12_4_1 e_1_2_12_6_1 e_1_2_12_19_1 e_1_2_12_2_1 e_1_2_12_17_1 e_1_2_12_38_1 e_1_2_12_41_1 e_1_2_12_22_1 e_1_2_12_43_1 e_1_2_12_26_1 e_1_2_12_47_1 Tannenbaum J. (e_1_2_12_45_1) 2015; 54 e_1_2_12_28_1 e_1_2_12_49_1 e_1_2_12_31_1 e_1_2_12_52_1 e_1_2_12_33_1 e_1_2_12_54_1 Kouchmeshky A. (e_1_2_12_16_1) 2020 e_1_2_12_35_1 e_1_2_12_37_1 Luft J.H. (e_1_2_12_20_1) 1964; 23 Meban C. (e_1_2_12_24_1) 1984; 139 e_1_2_12_14_1 e_1_2_12_12_1 e_1_2_12_8_1 e_1_2_12_10_1 e_1_2_12_50_1 e_1_2_12_3_1 e_1_2_12_5_1 e_1_2_12_18_1 e_1_2_12_39_1 e_1_2_12_42_1 e_1_2_12_21_1 e_1_2_12_44_1 e_1_2_12_23_1 e_1_2_12_46_1 e_1_2_12_25_1 e_1_2_12_48_1 e_1_2_12_40_1 e_1_2_12_27_1 e_1_2_12_29_1 e_1_2_12_30_1 e_1_2_12_53_1 e_1_2_12_32_1 e_1_2_12_34_1 e_1_2_12_36_1 e_1_2_12_15_1 e_1_2_12_13_1 e_1_2_12_11_1 e_1_2_12_7_1 e_1_2_12_51_1 e_1_2_12_9_1 |
| References_xml | – volume: 14 year: 2014 article-title: Gene silencing of β‐galactosamide α‐2,6‐sialyltransferase 1 inhibits human influenza virus infection of airway epithelial cells publication-title: BMC Microbiology – volume: 8 year: 2020 article-title: The emerging role of the mammalian glycocalyx in functional membrane organization and immune system regulation publication-title: Frontiers in Cell and Development Biology – volume: 167 start-page: 389 issue: 4 year: 1970 end-page: 400 article-title: Morphology of alveolar lining layer publication-title: The Anatomical Record – volume: 175 start-page: 1125 issue: 4469 year: 1955 end-page: 1126 article-title: Properties, function and origin of the alveolar lining layer publication-title: Nature – volume: 21 issue: 9 year: 2020 article-title: On top of the alveolar epithelium: surfactant and the glycocalyx publication-title: International Journal of Molecular Sciences – volume: 23 start-page: 54A year: 1964 end-page: 55A article-title: Electron microscopy of cell extraneous coats as revealed by ruthenium red staining publication-title: The Journal of Cell Biology – volume: 30 start-page: 1325 issue: 12 year: 1982 end-page: 1330 article-title: Lanthanum as an electron microscopic stain publication-title: The Journal of Histochemistry and Cytochemistry – volume: 4 start-page: 73 issue: 2 year: 1964 end-page: 85 article-title: The chemical and histochemical properties of Alcian blue publication-title: Histochemie – volume: 160 start-page: 83 issue: 2 year: 2023 end-page: 96 article-title: The unremarkable alveolar epithelial glycocalyx: a thorium dioxide‐based electron microscopic comparison after heparinase or pneumolysin treatment publication-title: Histochemistry and Cell Biology – volume: 454 start-page: 345 issue: 3 year: 2007 end-page: 359 article-title: The endothelial glycocalyx: composition, functions, and visualization publication-title: Pflügers Archiv – volume: 7 issue: 2 year: 2022 article-title: Alveolar epithelial glycocalyx degradation mediates surfactant dysfunction and contributes to acute respiratory distress syndrome publication-title: JCI Insight – volume: 127 start-page: 896 issue: 5 year: 1971 end-page: 902 article-title: Ultrastructure of lung fixed under physiologically defined conditions publication-title: Archives of Internal Medicine – volume: 11 start-page: 14 issue: 1 year: 1963 end-page: 23 article-title: Morphological aspects of extracellular polysaccharides publication-title: The Journal of Histochemistry and Cytochemistry – volume: 171 start-page: 347 issue: 3 year: 1971 end-page: 368 article-title: Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action publication-title: The Anatomical Record – start-page: 176 year: 2019 end-page: 194 – volume: 53 start-page: 419 issue: 2 year: 1973 end-page: 495 article-title: Morphological basis of alveolar‐capillary gas exchange publication-title: Physiological Reviews – volume: 4 start-page: 42 issue: 1 year: 1968 end-page: 57 article-title: Electron microscopic demonstration of an extracellular duplex lining layer of alveoli publication-title: Respiration Physiology – volume: 70 start-page: 549 issue: 2 year: 2013 end-page: 561 article-title: An improved method for preparing glutaraldehyde cross‐linked chitosan–poly(vinyl alcohol) microparticles publication-title: Polymer Bulletin – volume: 14 start-page: 117 issue: 2 year: 2017 end-page: 126 article-title: Endothelial glycocalyx as biomarker for cardiovascular diseases: mechanistic and clinical implications publication-title: Current Heart Failure Reports – volume: 133 year: 2021 article-title: Alveolar epithelial glycocalyx shedding aggravates the epithelial barrier and disrupts epithelial tight junctions in acute respiratory distress syndrome publication-title: Biomedicine & Pharmacotherapy – volume: 17 start-page: 208 issue: 1 year: 1963 end-page: 212 article-title: The use of lead citrate at high pH as an electron‐opaque stain in electron microscopy publication-title: The Journal of Cell Biology – volume: 150 start-page: 661 issue: 6 year: 2018 end-page: 676 article-title: The micromechanics of lung alveoli: structure and function of surfactant and tissue components publication-title: Histochemistry and Cell Biology – volume: 3 start-page: 95 issue: Suppl 2 year: 2004 end-page: 96 article-title: Altered terminal glycosylation and the pathophysiology of CF lung disease publication-title: Journal of Cystic Fibrosis – volume: 12 year: 2021 article-title: ST6GAL1 and α2‐6 sialylation regulates IL‐6 expression and secretion in chronic obstructive pulmonary disease publication-title: Frontiers in Immunology – volume: 101 start-page: 55 issue: 333 year: 2017 end-page: 63 article-title: Electron microscopy approach for the visualization of the epithelial and endothelial glycocalyx publication-title: Morphologie – volume: 134 start-page: 319 year: 2010 end-page: 326 article-title: Lamellar body ultrastructure revisited: high‐pressure freezing and cryo‐electron microscopy of vitreous sections publication-title: Histochemistry and Cell Biology – volume: 44 start-page: 253 issue: Suppl 1 year: 1983 end-page: 257 article-title: Effects of Thorotrast in humans publication-title: Health Physics – start-page: 208 year: 1996 end-page: 229 – year: 2019 – start-page: 429 year: 1998 end-page: 454 – volume: 66 start-page: 373 issue: 1 year: 1929 end-page: 394 article-title: Neue Auffassungen über einen Grundbegriff der Atemmechanik. Die Retraktionskraft der Lunge, abhängig von der Oberflächenspannung in den Alveolen publication-title: Zeitschrift für die gesamte experimentelle Medizin – volume: 146 start-page: 695 issue: 6 year: 2016 end-page: 707 article-title: Using electron microscopes to look into the lung publication-title: Histochemistry and Cell Biology – volume: 54 start-page: 120 issue: 2 year: 2015 end-page: 132 article-title: Russell and Burch's 3Rs then and now: the need for clarity in definition and purpose publication-title: Journal of the American Association for Laboratory Animal Science – volume: 216 start-page: 131 year: 2002 end-page: 173 article-title: Glycocalyx of lung epithelial cells publication-title: International Review of Cytology – volume: 324 start-page: c799 issue: 4 year: 2023 end-page: c806 article-title: The role of the alveolar epithelial glycocalyx in acute respiratory distress syndrome publication-title: American Journal of Physiology Cell Physiology – volume: 25 start-page: 23 issue: 1 year: 1965 end-page: 41 article-title: Diazophthalocyanins as reagents for fine structural cytochemistry publication-title: The Journal of Cell Biology – volume: 23 start-page: 325 issue: 3 year: 1975 end-page: 335 article-title: Electron microscopic demonstration of saccharide moieties in the hypophase of the alveolar surfactant system publication-title: Respiration Physiology – volume: 51 start-page: 611 issue: 3 year: 1971 end-page: 620 article-title: Lanthanum staining of the surface coat of cells. Its enhancement by the use of fixatives containing Alcian blue or cetylpyridinium chloride publication-title: The Journal of Cell Biology – volume: 25 start-page: 27 issue: 1 year: 2010 end-page: 40 article-title: The closer we look the more we see? Quantitative microscopic analysis of the pulmonary surfactant system publication-title: Cellular Physiology and Biochemistry – volume: 38 start-page: 233 issue: 2 year: 1990 end-page: 244 article-title: Lectin binding patterns to terminal sugars of rat lung alveolar epithelial cells publication-title: The Journal of Histochemistry and Cytochemistry – volume: 59 start-page: 363 issue: 3 year: 2018 end-page: 374 article-title: Epithelial heparan sulfate contributes to alveolar barrier function and is shed during lung injury publication-title: American Journal of Respiratory Cell and Molecular Biology – volume: 1 start-page: 500 year: 1960 article-title: The principles of humane experimental technique publication-title: The Medical Journal of Australia – volume: 139 start-page: 371 issue: Pt 2 year: 1984 end-page: 385 article-title: The surface coating of the pneumonocytes in human neonatal lung publication-title: Journal of Anatomy – volume: 280 start-page: 97 issue: 1 year: 2016 end-page: 113 article-title: The glycocalyx and its significance in human medicine publication-title: Journal of Internal Medicine – volume: 31 start-page: 987 issue: 8 year: 1983 end-page: 999 article-title: Application of lectin‐gold complexes for electron microscopic localization of glycoconjugates on thin sections publication-title: The Journal of Histochemistry and Cytochemistry – volume: 8 start-page: 13 issue: 1 year: 1969 end-page: 36 article-title: Improvements in demonstration of lining layer of lung alveoli by electron microscopy publication-title: Respiration Physiology – volume: 1778 start-page: 1676 issue: 7–8 year: 2008 end-page: 1695 article-title: Structure of pulmonary surfactant membranes and films: the role of proteins and lipid‐protein interactions publication-title: Biochimica et Biophysica Acta – volume: 27 start-page: 815 issue: 4 year: 2021 end-page: 827 article-title: Preparation of samples for large‐scale automated electron microscopy of tissue and cell ultrastructure publication-title: Microscopy and Microanalysis – volume: 4 start-page: 1 issue: 1 year: 1972 end-page: 8 article-title: An electron microscopic study of the acid mucosubstance lining the alveoli of hamster lung publication-title: The Histochemical Journal – start-page: 119 year: 2020 end-page: 150 – volume: 35 start-page: 959 issue: 9 year: 1987 end-page: 969 article-title: The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions publication-title: The Journal of Histochemistry and Cytochemistry – volume: 79 start-page: 1615 issue: 5 year: 1995 end-page: 1628 article-title: Alveolar lining layer is thin and continuous: low‐temperature scanning electron microscopy of rat lung publication-title: Journal of Applied Physiology – volume: 27 start-page: 47 issue: 1 year: 1987 end-page: 65 article-title: A newly identified surface coat on cochlear hair cells publication-title: Hearing Research – ident: e_1_2_12_31_1 doi: 10.1007/s00424‐007‐0212‐8 – ident: e_1_2_12_48_1 doi: 10.1007/s00418‐023‐02211‐7 – ident: e_1_2_12_2_1 doi: 10.1152/jappl.1995.79.5.1615 – ident: e_1_2_12_12_1 doi: 10.1177/35.9.3302020 – ident: e_1_2_12_30_1 doi: 10.1016/j.bbamem.2008.05.003 – ident: e_1_2_12_35_1 doi: 10.1152/ajpcell.00555.2022 – ident: e_1_2_12_32_1 doi: 10.1083/jcb.17.1.208 – ident: e_1_2_12_21_1 doi: 10.1002/ar.1091710302 – ident: e_1_2_12_25_1 doi: 10.3389/fcell.2020.00253 – ident: e_1_2_12_41_1 doi: 10.1177/30.12.6185564 – ident: e_1_2_12_11_1 doi: 10.1165/rcmb.2017‐0428OC – ident: e_1_2_12_38_1 doi: 10.5694/j.1326‐5377.1960.tb73127.x – ident: e_1_2_12_43_1 doi: 10.1097/00004032‐198306001‐00022 – ident: e_1_2_12_47_1 doi: 10.1083/jcb.25.1.23 – start-page: 119 volume-title: Methods in enzymology year: 2020 ident: e_1_2_12_16_1 – ident: e_1_2_12_44_1 doi: 10.1177/38.2.1688898 – ident: e_1_2_12_46_1 doi: 10.1111/joim.12465 – ident: e_1_2_12_51_1 doi: 10.1152/physrev.1973.53.2.419 – ident: e_1_2_12_37_1 doi: 10.1177/31.8.6190857 – ident: e_1_2_12_42_1 doi: 10.1083/jcb.51.3.611 – ident: e_1_2_12_50_1 doi: 10.1007/BF02621963 – ident: e_1_2_12_22_1 doi: 10.1016/s0074‐7696(02)16005‐0 – ident: e_1_2_12_36_1 doi: 10.1016/0034‐5687(75)90083‐3 – volume: 139 start-page: 371 issue: 2 year: 1984 ident: e_1_2_12_24_1 article-title: The surface coating of the pneumonocytes in human neonatal lung publication-title: Journal of Anatomy – ident: e_1_2_12_53_1 – ident: e_1_2_12_26_1 doi: 10.1159/000272061 – ident: e_1_2_12_29_1 doi: 10.1038/1751125b0 – volume: 23 start-page: 54A year: 1964 ident: e_1_2_12_20_1 article-title: Electron microscopy of cell extraneous coats as revealed by ruthenium red staining publication-title: The Journal of Cell Biology – ident: e_1_2_12_54_1 doi: 10.1186/1471‐2180‐14‐78 – ident: e_1_2_12_13_1 doi: 10.1002/ar.1091670403 – ident: e_1_2_12_52_1 doi: 10.1016/0034‐5687(68)90006‐6 – volume: 54 start-page: 120 issue: 2 year: 2015 ident: e_1_2_12_45_1 article-title: Russell and Burch's 3Rs then and now: the need for clarity in definition and purpose publication-title: Journal of the American Association for Laboratory Animal Science – ident: e_1_2_12_9_1 doi: 10.1001/archinte.1971.00310170104014 – ident: e_1_2_12_10_1 doi: 10.1016/0034‐5687(69)90042‐5 – ident: e_1_2_12_19_1 doi: 10.1016/j.biopha.2020.111026 – ident: e_1_2_12_8_1 doi: 10.1007/978-3-0348-8855-4_17 – ident: e_1_2_12_14_1 doi: 10.1007/s11897‐017‐0320‐5 – ident: e_1_2_12_15_1 doi: 10.1007/s00418‐018‐1747‐9 – ident: e_1_2_12_23_1 doi: 10.1007/BF01005264 – ident: e_1_2_12_17_1 doi: 10.3389/fimmu.2021.693149 – ident: e_1_2_12_3_1 doi: 10.1177/11.1.14 – ident: e_1_2_12_27_1 doi: 10.3390/ijms21093075 – ident: e_1_2_12_28_1 doi: 10.1007/s00418‐016‐1502‐z – ident: e_1_2_12_4_1 doi: 10.1007/s00289‐012‐0853‐4 – ident: e_1_2_12_5_1 doi: 10.1016/j.morpho.2017.04.001 – ident: e_1_2_12_39_1 doi: 10.1016/0378‐5955(87)90025‐6 – ident: e_1_2_12_34_1 doi: 10.1172/jci.insight.154573 – ident: e_1_2_12_33_1 doi: 10.1016/j.jcf.2004.05.021 – ident: e_1_2_12_7_1 doi: 10.1017/S1431927621011958 – ident: e_1_2_12_40_1 doi: 10.1007/BF00306149 – ident: e_1_2_12_6_1 doi: 10.1007/978-1-4614-7520-0_7 – ident: e_1_2_12_18_1 doi: 10.1016/B978-0-7020-6864-5.00013-X – ident: e_1_2_12_49_1 doi: 10.1007/s00418‐010‐0736‐4 |
| SSID | ssj0013094 |
| Score | 2.4453104 |
| Snippet | The alveolar surface of the lung is lined by an epithelium consisting of type I (AECI) and type II alveolar epithelial cells (AECII). This epithelium is... |
| SourceID | pubmedcentral proquest pubmed crossref wiley |
| SourceType | Open Access Repository Aggregation Database Index Database Publisher |
| StartPage | 770 |
| SubjectTerms | alcian blue Aldehydes Alveolar Epithelial Cells - ultrastructure alveolar epithelial glycocalyx Alveoli Animal welfare Animals Embedding Epithelial cells Epithelium Fucose Glycocalyx - ultrastructure Labeling Lanthanum lectin Lectins Mice Mice, Inbred C57BL Microscopy, Electron, Transmission Original Perfusion Pulmonary Alveoli - ultrastructure Ruthenium red sialic acid Staining and Labeling - methods Transmission electron microscopy Visualization |
| Title | Comparative electron microscopic visualization of the lung alveolar epithelial glycocalyx with different staining and labeling methods |
| URI | https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fjoa.14129 https://www.ncbi.nlm.nih.gov/pubmed/39245632 https://www.proquest.com/docview/3190013959 https://www.proquest.com/docview/3102070124 https://pubmed.ncbi.nlm.nih.gov/PMC11996704 |
| Volume | 246 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bS9xAFD6oUPHFtl7qtlam4oMvkVwmySx9ElsRQSuiICKEycyJXbpNpLsr3f6A_u6eM7noVgrFt5CZJJPMuXwn5wawIw0JPEISnim09KSNck8TkPCUJeyPGpNAc4LzyWlydCmPr-KrOfjY5sLU9SG6H27MGU5eM4PrfPSYyStNbE7qiuRvECVcN__TefjgQfD7TQXmgDhehU1VIRfF0145q4ueAMyncZKP8atTQIcv4aZdeh138m1vMs73zK-_qjo-891ewXIDTMV-TUmvYQ7LFXhRt6qcrsDiSeOEp5PXlTu5Cr8PHmqHi7ahjvjOMX6c7TIw4n4w4qzNOtdTVIUgvCmGJGCEHt4jm9UC7zgtZEh8IG6HU8O6dfpT8P9h0XZvGQuX5TXgy0oriHBdFr2o21-P1uDy8PPFwZHXNHbwjCSLxDOWUJGV1qjEt1ho1Kml_dKhr_omL9I0jlUQqdxHHWEU6SJIkEwbRcYqBnmO0ToslFWJGyAk4U8y6UxiYykLZK-m0uwM7Ut2EWIPttstzu7q-h1ZZ_dUOnNfuQeb7eZnDQuPMpJNDh_HNPyhGybmY4-KLrGa8BxC2ynpeNmDNzWtdE8h4EngNAp7oGaoqJvAhb1nR8rBV1fgO-DQ8NSnm-46Kvn3yrPjL_vu4O3_T30HSyE3MXZRm5uwMP4xwfeErMb5FsyH8mzLMdIfB-oizg |
| linkProvider | Wiley-Blackwell |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1LT9wwEB4BVUsvqIU-tkDrVj1wiZSHk3glLqsVaEtZ2gNIqJfIsSd0pW2C2AWxf4Df3RnnASuExC2ynYcyM55vPC-A79LQhkdIwjOFlp60Ue5pAhKesoT9UWMSaE5wHp8kozN5dB6fr8B-mwtT14foDtxYMtx-zQLOB9IPpbzSJOekr1bhhSRczgF9ofx970Pw-00N5oBkXoVNXSEXx9PeuqyNHkHMx5GSDxGsU0GHb2CjwY5iUBP7LaxguQkv626Si014NW785DT4p3KDW3A3vC_vLdqeN-Ifh-FxQsrEiJvJjBMr63RMURWCIKGY0h4g9PQG2fIVeMmZG1NiVXExXRhWf4tbwUe4om2wMhcuEWvCt5VWEG-5RHdRd6ievYOzw4PT4chrei94RpLR4BlLwMVKa1TiWyw06tTSD9Whr_omL9I0jlUQqdxHHWEU6SJIkKwPRfYkBnmO0XtYK6sSP4KQBBHJ6jKJjaUskB2PSrO_si_Zi4c9-NbSILusS2xknWlS6cwRqgc7LXWyRspmGW0fDsLGNP21myb5YKeHLrG65jUEiFNSw7IHH2pidm8hbEj4MQp7oJbI3C3g2tvLM-Xkr6vBHXD0durTQ_ccRzz95dnRr4G7-PT8pV9gfXQ6Ps6Of5z83IbXIfccdkGWO7A2v7rGXQJC8_yz4_f_Mr0FZg |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3dT9RAEJ8gRuKLKAqeoq7GB19K-rFt9-ITAS-IgsZIQoxJs92d4sWzvXh3hOMP4O9mZvsBJzExvl26217bnd_ObzpfAK-loQ2PmIRnCi09aaPc00QkPGWJ-6PGJNCc4HxwmOwdyf3j-HgJ3ra5MHV9iO6DGyPD7dcM8LEtroO80gRzUle34LZMiEkwI_oSXrkQ_H5TgjkgyKuwKSvkwnjaUxeV0Q2GeTNQ8jqBdRposArf23uvA09-bs2m-ZY5_6Os438-3H241zBTsV2L0gNYwnIN7tS9KudrsHLQeOHp4LfKHXwIFztXxcNF21FH_OIgP053GRpxOpxw2mad7CmqQhDhFCPaYYQenSLb1QLHnBcyIiCIk9HcsHKdnwn-QCza9i1T4dK8hnxaaQVJrkujF3X_68kjOBq8-7qz5zWdHTwjySTxjCVaZKU1KvEtFhp1amm9dOirvsmLNI1jFUQq91FHGEW6CBIk20aRtYpBnmO0DstlVeJjEJIIKNl0JrGxlAWyW1Np9ob2JfsIsQev2iXOxnUBj6wzfCqdubfcg8128bMGw5OMNidHkGMaftkNE_rYpaJLrGY8h-h2Skpe9mCjlpXuX4h5EjuNwh6oBSnqJnBl78WRcvjDVfgOODY89emib5yU_P3Os_1P2-7Hk3-f-gJWPu8Oso_vDz88hbshNzR2EZybsDz9PcNnxLKm-XOHpku9hiTF |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Comparative+electron+microscopic+visualization+of+the+lung+alveolar+epithelial+glycocalyx+with+different+staining+and+labeling+methods&rft.jtitle=Journal+of+anatomy&rft.au=Gluhovic%2C+Vladimir&rft.au=Timm%2C+Sara&rft.au=Kuebler%2C+Wolfgang+M&rft.au=Lopez-Rodriguez%2C+Elena&rft.date=2025-05-01&rft.eissn=1469-7580&rft.volume=246&rft.issue=5&rft.spage=770&rft_id=info:doi/10.1111%2Fjoa.14129&rft_id=info%3Apmid%2F39245632&rft.externalDocID=39245632 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0021-8782&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0021-8782&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0021-8782&client=summon |