Analysis and bioequivalency study on two tablet formulations of co-trimoxazole

• Published in: Journal of clinical pharmacy and therapeutics Vol. 18; no. 2; p. 109
• Main Authors: Sheikh Salem, M, Alkaysi, H N, Gharaibeh, A M, Amari, F F, Sallam, E, Shubair, M S
• Format: Journal Article
• Language: English
• Published: England 01.04.1993
• Subjects: Biological Availability; Chromatography, High Pressure Liquid - methods; Humans; Male; Reproducibility of Results; Sensitivity and Specificity; Tablets; Therapeutic Equivalency; Trimethoprim, Sulfamethoxazole Drug Combination - blood; Trimethoprim, Sulfamethoxazole Drug Combination - pharmacokinetics
• ISSN: 0269-4727
• DOI: 10.1111/j.1365-2710.1993.tb00576.x

This paper describes an analytical procedure for simultaneous quantification of trimethoprim (TMP) and sulphamethoxazole (SMZ) in serum. Serum samples were rendered alkaline with glycine-sodium hydroxide buffer (pH 9.1) and extracted with ethyl acetate. The reconstituted sample was analysed using reversed-phase C-18 column high-performance chromatography. The mobile phase consisted of 75% triethyl ammonium acetate buffer, 20% methanol and 5% acetonitrile. The analytes were monitored at 289 nm and tetroxoprim was used as an internal standard. The maximum values for intra-day coefficients of variation (CV) for TMP-and SMZ were 5.7 and 2.1%, respectively. Interday CV values were 6.4 and 2.2%, respectively. The method was used to compare the bioavailability of two tablet formulations in terms of their pharmacokinetic parameters following oral administration of the tablets to 18 volunteers.