Analysis of mouse β-haptoglobin chain by lectin affinoblotting detection

• Published in: Electrophoresis Vol. 22; no. 14; pp. 3038 - 3042
• Main Authors: Roemer, Irmgard, Vogel, Tilman, Otto, Albrecht, Fichtner, Iduna, Klose, Joachim
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.08.2001
• Subjects: Acute-Phase Proteins - analysis; Acute-Phase Proteins - chemistry; Acute-Phase Proteins - genetics; Animals; Breast Neoplasms - blood; Coloring Agents; Electrophoresis, Gel, Two-Dimensional; Glycoproteins - analysis; Glycoproteins - blood; Glycoproteins - chemistry; Glycoproteins - genetics; Glycosylation; Haptoglobin; Humans; Lectins; Mice; Mice, Nude; Mouse; Neoplasm Proteins - blood; Neoplasm Transplantation; Polysaccharides - analysis; Protein Processing, Post-Translational; Silver Staining; Stress, Physiological - blood; Transplantation, Heterologous; Tumor transplantation; Two-dimensional electrophoresis

Two different human mammary carcinoma cell lines were xenotransplanted into nude mice. Serum samples were obtained prior to and after transplantation and investigated by two‐dimensional electrophoresis (2‐DE). By comparison of these silver‐stained patterns additional protein spots were detected resu...