Different temperature sensitivity of endosomes involved in transport to lysosomes and transcytosis in rat hepatocytes: Analysis by free-flow electrophoresis

• Published in: Electrophoresis Vol. 23; no. 13; pp. 2117 - 2129
• Main Authors: Ellinger, Isabella, Klapper, Herbert, Courtoy, Pierre J., Vaerman, Jean-Pierre, Fuchs, Renate
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag GmbH 01.07.2002; WILEY‐VCH Verlag GmbH
• Subjects: Animals; Asialoglycoproteins - analysis; Bile - secretion; Biological Transport; Cell Fractionation - methods; Electrophoresis - methods; Endosomes; Endosomes - physiology; Endosomes - ultrastructure; Fluorescein-5-isothiocyanate; Free-flow electrophoresis; Hepatocytes; Hepatocytes - cytology; Hepatocytes - physiology; Isolated perfused rat liver; Liver - cytology; Liver - physiology; Lysosomes - physiology; Lysosomes - ultrastructure; Orosomucoid - analogs & derivatives; Orosomucoid - analysis; Perfusion; Rats; Spectrometry, Fluorescence - methods; Temperature; Temperature sensitivity

Endocytosis at reduced temperature has been used to define and characterize endosome subpopulations. Thus, the temperature sensitivity of endosome subpopulations involved in transport to lysosomes and transcytosis in rat hepatocytes was analyzed applying endosome labeling in the isolated perfused ra...