Opposite effects of Zn on the in vitro binding of [3H]LY354740 to recombinant and native metabotropic glutamate 2 and 3 receptors

• Published in: Journal of neurochemistry Vol. 94; no. 1; pp. 150 - 160
• Main Authors: Malherbe, Pari, Richards, J. Grayson, Broger, Clemens, Zenner, Marie‐Thérèse, Messer, Jürg, Kratzeisen, Claudia, Nakanishi, Shigetada, Mutel, Vincent
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.07.2005; Blackwell; Blackwell Publishing Ltd
• Subjects: Amino Acid Sequence; Animals; Binding Sites - genetics; Biochemistry; Biological and medical sciences; Brain; Bridged Bicyclo Compounds - metabolism; Cell Line; Cell receptors; Cell structures and functions; Central nervous system; Central neurotransmission. Neuromudulation. Pathways and receptors; Chlorides - chemistry; Chlorides - physiology; Excitatory Amino Acid Agonists - metabolism; Fundamental and applied biological sciences. Psychology; Humans; knockout; metabotropic glutamate receptor; Mice; Mice, Knockout; Molecular and cellular biology; Molecular Sequence Data; Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine); mutagenesis/chimeric approaches; Neurology; Point Mutation; Protein Binding - genetics; Rats; Receptors, Metabotropic Glutamate - agonists; Receptors, Metabotropic Glutamate - genetics; Receptors, Metabotropic Glutamate - metabolism; receptor‐binding studies; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Rodents; Tritium - metabolism; Vertebrates: nervous system and sense organs; Zinc; Zinc Compounds - chemistry; Zn effect; knockout; Zn effect; metabotropic glutamate receptor; Rodentia; Central nervous system; Glutamate receptor; In vitro; mglu2 glutamate receptor; mglu3 glutamate receptor; Section; Group II mglu glutamate receptor; Zinc; Encephalon; Vertebrata; Mammalia; Mutagenesis; Metabotropic receptor; Mouse; receptor-binding studies; mutagenesis/chimeric approaches; Hippocampus; Biological receptor

We investigated the effect of Zn on agonist binding to both recombinant and native mGlu2 and mGlu3 receptors. Zn had a biphasic inhibitory effect on recombinant mGlu2 with IC50 values for the high‐ and low‐affinity components of 60 ± 10 µm and 2 ± 0.7 mm, respectively. Zn induced a complex biphasic...