Gβγ directly modulates vesicle fusion by competing with synaptotagmin for binding to neuronal SNARE proteins embedded in membranes

• Published in: The Journal of biological chemistry Vol. 292; no. 29; pp. 12165 - 12177
• Main Authors: Zurawski, Zack, Page, Brian, Chicka, Michael C., Brindley, Rebecca L., Wells, Christopher A., Preininger, Anita M., Hyde, Karren, Gilbert, James A., Cruz-Rodriguez, Osvaldo, Currie, Kevin P.M., Chapman, Edwin R., Alford, Simon, Hamm, Heidi E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.07.2017; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Binding, Competitive; Calcium Signaling; Cattle; Cell Line; exocytosis; G protein; G protein-coupled receptor (GPCR); GTP-Binding Protein beta Subunits - chemistry; GTP-Binding Protein beta Subunits - genetics; GTP-Binding Protein beta Subunits - metabolism; GTP-Binding Protein gamma Subunits - chemistry; GTP-Binding Protein gamma Subunits - genetics; GTP-Binding Protein gamma Subunits - metabolism; Humans; Lipid Bilayers; Liposomes; Membrane Fusion; Models, Molecular; Mutation; Nerve Tissue Proteins - chemistry; Nerve Tissue Proteins - metabolism; neurotransmitter release; Peptide Fragments - chemistry; Peptide Fragments - genetics; Peptide Fragments - metabolism; Protein Conformation; Protein Interaction Domains and Motifs; Protein Multimerization; Rats; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - metabolism; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism; Signal Transduction; SNARE proteins; Synaptosomal-Associated Protein 25 - chemistry; Synaptosomal-Associated Protein 25 - metabolism; Synaptotagmin I - chemistry; Synaptotagmin I - genetics; Synaptotagmin I - metabolism; Syntaxin 1 - chemistry; Syntaxin 1 - metabolism; G protein-coupled receptor (GPCR); exocytosis; neurotransmitter release; SNARE proteins; G protein

Gi/o-coupled G protein-coupled receptors can inhibit neurotransmitter release at synapses via multiple mechanisms. In addition to Gβγ-mediated modulation of voltage-gated calcium channels (VGCC), inhibition can also be mediated through the direct interaction of Gβγ subunits with the soluble N-ethylm...