Distinctive role of fluG in the adaptation of Beauveria bassiana to insect‐pathogenic lifecycle and environmental stresses

• Published in: Environmental microbiology Vol. 23; no. 9; pp. 5184 - 5199
• Main Authors: Guo, Chong‐Tao, Peng, Han, Tong, Sen‐Miao, Ying, Sheng‐Hua, Feng, Ming‐Guang
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.09.2021; Wiley Subscription Services, Inc
• Subjects: Adaptation; Aspergillus; Beauveria bassiana; blastospores; conidiation; Cuticles; death; Environmental stress; Epicuticle; Fungi; Gene regulation; Genes; Haemolymph; Hemolymph; hyphae; Incubation period; Insects; Mimicry; Pathogens; Proliferation; Secretion; Stresses; Virulence
• ISSN: 1462-2912; 1462-2920; 1462-2920
• DOI: 10.1111/1462-2920.15500

Summary The upstream developmental activation (UDA) pathway comprises three fluG‐cored cascades (fluG‐flbA, fluG‐flbE/B/D and fluG‐flbC) that activate the key gene brlA of central developmental pathway (CDP) to initiate conidiation in aspergilli. However, the core role of fluG remains poorly understood in other fungi. Here, we report distinctive role of fluG in the insect‐pathogenic lifecycle of Beauveria bassiana. Disruption of fluG resulted in limited conidiation defect, which was mitigated with incubation time and associated with time‐course up‐regulation/down‐regulation of all flb and CDP genes and another fluG‐like gene (BBA_06309). In ΔfluG, increased sensitivities to various stresses correlated with repression of corresponding stress‐responsive genes. Its virulence through normal cuticle infection was attenuated greatly due to blocked secretion of cuticle‐degrading enzymes and delayed formation of hyphal bodies (blastospores) to accelerate proliferation in vivo and host death. In submerged ΔfluG cultures mimicking insect haemolymph, largely increased blastospore production concurred with drastic up‐regulation of the CDP genes brlA and abaA, which was associated with earlier up‐regulation of most flb genes in the cultures. Our results unveil an essentiality of fluG for fungal adaptation to insect‐pathogenic lifecycle and suggest the other fluG‐like gene to act as an alternative player in the UDA pathway of B. bassiana.